Cell cycle progression analysis
ANALYSIS OF TWO PLANT PROTEIN COMPLEXES ASSOCIATED WITH TRANSCRIPTION AND CELL CYCLE PROGRESSION
... Atccs52B. Analysis of the Atccs52 gene functions in ...of cell proliferation and at the same time in elongation of yeast cells and enlargement of ...phenotypes; cell growth in addition to elongation ...
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Regulation of genome stability and cell cycle progression by SUMOylation
... cellular analysis by microcopy, the SUMOylation state of the PML protein after depletion of endogenous USP11 in HeLa cells was ...HeLa cell lines stably expressing His-tagged SUMO-2 were used to allow for ...
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Discovery and analysis of long noncoding RNAs in gene expression control and cell cycle progression
... in cell cycle ...replication, cell proliferation, oncogenic transformation and ...promotes cell proliferation, was also downregulated in S7-depleted cells (Figure ...
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Predicting stem cell fate changes by differential cell cycle progression patterns
... Single cell analysis and tracking of FUCCI-NSC allowed us to identify cell cycle parameters that correlate with the extent of differentiation. We used the accumulation of Cdt1-KO2 to ...
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Bayesian Statistical Models of Cell-Cycle Progression at Single-Cell and Population Levels
... as cell density in a field of view increases, the ability to track in- dividual cells and cell lineages ...for analysis. Increasing cell density over the course of a time-lapse movie doesn’t ...
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A Fungal Arrestin Protein Contributes to Cell Cycle Progression and Pathogenesis
... IMPORTANCE To survive under unpredictable conditions, all organisms must adapt to stressors by regulating adaptive cellular responses. Arrestin proteins are con- served regulators of adaptive cellular responses in ...
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Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression
... To determine the protein synthesis rate, the cells were then washed, trypsinized, centrifuged at 3,000 x g, and resus- pended in serum-free medium. Aliquots were taken for cell- number analysis in a Coulter ...
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Metformin inhibits cell cycle progression of B-cell chronic lymphocytic leukemia cells
... G0/G1 cell cycle phase and show a gene expression profile of resting cells ...B cell survival and ...[6–8]. Analysis of these subpopulations revealed a spectrum of leukemic cells ranging from ...
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Dap160/intersectin binds and activates aPKC to regulate cell polarity and cell cycle progression
... Statistical analysis To test the significance in neuroblast number variation between wild-type, dap160 mutant and Dap160-overexpressing brains we used a t-test (two- tailed distribution and two samples, equal ...
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Checkpoints couple transcription network oscillator dynamics to cell cycle progression
... In order to determine whether oscillations are perpetu- ated via the MBF or SBF branch of the network in Cdc20- depleted cells, we closely examined the behaviors of genes that have been annotated as MBF- or ...
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Diabetic LDL inhibits cell cycle progression via STAT5B and p21waf
... a cell cycle regulator known to be a target of the signal transducers and activators of transcription ...immunofluorescence analysis demonstrated that activated STAT5 is expressed in newly formed ...
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AP4 is required for mitogen- and c-MYC-induced cell cycle progression
... By analysis of AP4-deficient MEFs we were able to identify new and important cellular functions of the bHLH-LZ transcription factor ...c-MYC-induced cell cycle re-entry and mitogen-induced ...
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Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells
... (FCM) analysis The effect of lobaplatin on human HCC cell cycle distri- bution was determined by FCM ...USA). Cell fluorescence was measured in duplicate at each time point and all experi- ...
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Prolactin-Induced Protein Is Required for Cell Cycle Progression in Breast Cancer
... cancer cell lines, analysis of expression microarray data, proteomic analysis using mass spectrometry, and biomarker studies on breast ...the progression through G1 phase, mitosis, and ...
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IGFBP7 inhibits cell proliferation by suppressing AKT activity and cell cycle progression in thyroid carcinoma
... ATC cell lines and moderately expressed in FTC cell ...FTC cell lines, suggesting the important role for the loss of IGFBP7 expression during thyroid cancer development and pro- ...blotting ...
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Impaired germ cell development due to compromised cell cycle progression in Skp2-deficient mice
... microscopic analysis, tissue samples were fixed in either Bouin's fixative or 4% paraformaldehyde, dehy- drated, treated with xylene, embedded in paraffin, and sectioned at a thickness of 5 ...
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Neurogenin 2 regulates progenitor cell cycle progression and Purkinje cell dendritogenesis in cerebellar development
... and analysis of double (Neurog1/Neurog2) and triple (Neurog1/Neurog2/Ascl1) mutants to establish the roles, if any, of proneural genes in PC fate ...Further analysis is required to determine whether ...
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Analysis of Cell Cycle by Flow Cytometry
... Fig. 2. Bivariate distributions (scatterplots) showing the characteristic pattern of expression of cyclins D1, E, A, and B1 vs DNA content in normal, nontumor cells, processed as described in the protocol (2). Cyclin D1 ...
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Cell-cycle-phase progression analysis identifies unique phenotypes of major prognostic and predictive significance in breast cancer.
... novel cell-cycle algorithm to breast cancer has revealed major insights into the cell-cycle kinetics of this ...mitotic cell division cycle. These cell-cycle ...
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The Paramyxovirus Simian Virus 5 V Protein Slows Progression of the Cell Cycle
... prolonged cell cycle. To analyze the point in the cell cycle where the delay to cell proliferation occurred, cell cycle analysis of mock- and SV5- infected HeLa T4 ...
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