cell-free enzyme systems
Engineering Biosynthetic Pathways in Cell-Free Systems for Sustainability and Chemical Innovation
... non-natural enzyme activity that may be otherwise impossible in ...Collectively, cell-free systems accelerate the design-build-test cycle for engineering biosynthesis ...the ...
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Cell-free expression systems for the construction of artificial cells
... core enzyme and σ 70 transcription factor were mixed in an in vitro transcription reaction to reconstitute the RNA polymerase ...core enzyme and the σ 70 transcription factor, ...core enzyme is ...
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Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems
... for cell-free expression (enzymes, dNTPs, amino acids, ...paper-based cell-free diagnostic system that detected Norovirus with toehold switches [76] (Figure ...LacZ enzyme to improve ...
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Identification of a botanical inhibitor of intestinal diacylglyceride acyltransferase 1 activity via in vitro screening and a parallel, randomized, blinded, placebo-controlled clinical trial
... DGAT1 enzyme activity in vitro ...both cell-free and cellular model systems: apple peel extract (APE), grape extract (GE), red raspberry leaf extract (RLE) and apricot/nectarine extract (ANE) ...
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On the Uplink Max-Min SINR of Cell-Free Massive MIMO Systems
... [4]–[9]. Cell-free Massive MIMO is a combination of distributed MIMO and Massive MIMO, and there is no cell boundary ...[4]. Cell-free Massive MIMO is thus also a scalable version of ...
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Inhibition of Protein Synthesis in Cell-Free Systems from Interferon-Treated, Infected Cells: Further Characterization and Effect of Formylmethionyl-tRNAF
... Characterization of the polypeptide products synthesized in response to EMC RNA with time in the cell-free systems in the presence of cell sap fractions from vaccinia virus-infected and [r] ...
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OPTIMIZATION OF CHITINASE PRODUCTION BY BACTERIA FROM NOVEL ENDOPHYTIC NICHE OF GLORIOSA SUPERBA AND SOIL OF NORTH WESTERN REGION OF INDIA
... Farmers today are facing losses of billions of dollars due to damage to crops resulting from infection by fungi pathogens and attack by insect pests such as the pink bollworm, thrips, mites etc. They often resort to the ...
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The occurrence of chitinase in some bacteria
... Roseman (1954) reported the presence in cell-free extracts of Escherichia coli of an enzyme which de- acetylated acetylglucosamine; the optimum pH value was 8-3, no act[r] ...
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INTRACELLULAR ORGANIZATION OF ENZYME SYSTEMS
... Electron micrograph of the endoplasmic reticulum within a cell. Several oval niitochondria[r] ...
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Engineering Enzyme Systems by Recombination
... We constructed individual NNK libraries for each of these sites, and expressed mutants in E. coli BL21(DE3) ΔIlvD. We screened 90 variants from each library, using a medium throughput assay involving colorimetric ...
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The glycolytic oscillator
... The demonstration of oscillations in glycolysis of yeast cells and in cell-free extracts of yeast was the first indication that periodic activities of biological systems might be caused [r] ...
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Modeling enzyme processivity reveals that RNA Seq libraries are biased in characteristic and correctable ways
... protocol, free from any coverage bias, similar also to earlier assumptions of uniform coverage of RNA-seq data (using the measure of fragments per kilobase per million total fragments [FPKM]; see ...
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A study of the cell wall associated proteinase of lactic streptococci : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in biochemistry at Massey University
... The remarkable similarity of the properties of this enzyme with the enzyme released in Ca++ free- buffer see section 1.4 together with the evidence presented in this section all point to[r] ...
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DIVERSE BELONGINGS OF CALENDULA OFFICINALIS: AN OVERVIEW
... Jimenez et al. evaluated the cytotoxic anti-tumor activity and lymphocyte activation of the whole plant extract of Calendula officinalis. The in vitro cytotoxic anti-tumor and immunomodulatory activities and in vivo ...
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BIOCHEMICAL AND IN-VIVO ANTIOXIDANT PARAMETERS FOR EVALUATION OF MEMORY ENHANCING ACTIVITY
... In this method, the reaction mixture consists of 0.5ml supernatant, 1ml of 50mM Sodium carbonate, 0.4ml of 25µM NBT, 0.2ml of 0.1mM EDTA. The reaction is then initiated by the addition of 0.4ml of 1mM hydroxylamine ...
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Attempts to convert the cellular prion protein into the scrapie isoform in cell-free systems.
... Incubation of MHM2 PrPC with PrP 27-30 in vitro under various conditions for 24 h Formation of: Incubation conditions PrPsc PrPc Additional treatment MoPrP 27-30 MoPrP 27-30 MoPrP 27-30 [r] ...
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Activity and Stability of Urease Enzyme Immobilized on Amberlite Resin
... immobilized enzyme has lower catalytic activity comparing with free enzyme, it has more stability, reusability, and low cost effective 21,22 ...urease enzyme needed a chemical cross-linking ...
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Activation of the respiratory burst enzyme from human neutrophils in a cell free system Evidence for a soluble cofactor
... a cell-free system for activation of NADPH oxidase, the respiratory burst enzyme, in human ...This cell-free system can now be used to provide further insight into the biochemical basis ...
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Amyloglucosidase enzymatic reactivity inside lipid vesicles
... The enzyme of choice for these experiments is Amyloglucosidase ...This enzyme catalyzes both exo-(1–4) and branch-point (1–6)-linkages to produce glucose, provid- ing the primary step in the conversion of ...
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Galactose oxidase nanoaggregates: Preparation and characterization
... These enzyme nanoagregates have been characterized by transmission electron microscopy(TEM), UV visible spectroscopy, Fourier transform infrared spectroscopy ...
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