Cell-free systems
Plasmodium vivax: comparison of immunogenicity among proteins expressed in the cell free systems of Escherichia coli and wheat germ by suspension array assays
... Protein arrays containing hundreds to thousands of malarial proteins have been recently reported for anti- gen discovery [13,15,16]. In these experiments, in vitro transcribed/translated products are directly spotted ...
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Engineering Biosynthetic Pathways in Cell-Free Systems for Sustainability and Chemical Innovation
... Collectively, cell-free systems accelerate the design-build-test cycle for engineering biosynthesis ...the cell-free TX-TL ...the cell-free TX-TL system and presents a ...
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Inhibition of Protein Synthesis in Cell-Free Systems from Interferon-Treated, Infected Cells: Further Characterization and Effect of Formylmethionyl-tRNAF
... Characterization of the polypeptide products synthesized in response to EMC RNA with time in the cell-free systems in the presence of cell sap fractions from vaccinia virus-infected and [r] ...
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Translation of murine leukemia virus RNA in cell-free systems from animal cells.
... Fingerprint analysis of tryptic digests of polypeptide products formed in the cell-free system in B Polypeptides 60 and 70K synthesized in the L-cell-free system to 140,000-molecular-wei[r] ...
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Formation of Protease-Resistant Prion Protein in Cell-Free Systems
... The ultimate answers to these questions are not yet clear, but several relevant points can be made. Although the characteristic partial PK-resistance of PrP-res appears to faithfully reflect a specific abnormal ...
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Differential translation in normal and adenovirus type 5-infected human cells and cell-free systems.
... The change in polypeptide IX was quite striking; this polypeptide represented 5% of the total labeled protein obtained in isotonic medium, but its incidence rose to account for as much a[r] ...
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Protein Synthesis in Cell-Free Systems: an Effect of Interferon
... Stimulation of amino acid incorporation by EMC RNA with untreated "74S" ribosomes from control chick embryo fibroblasts CEF, U and CEF which had been exposed to 35 0 and 140 A units of i[r] ...
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AMINO ACID INCORPORATION CAPACITY OF CELL-FREE SYSTEMS FROM EMBRYONIC AXES OF PHASEOLUS COCCINEUS V.
... Cell-free protein synthesising systems, containing cytoplasmic ribosomes and 105,000 g supernatant were prepared from ungerminated (0 hr) axes and 12 he germinated axes of [r] ...
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Synthesis of Capsid and Noncapsid Viral Proteins in Response to Encephalomyocarditis Virus Ribonucleic Acid in Animal Cell-Free Systems
... From such a comparison, Smith, Marcker, and Mathews found no evidence for the cell-free synthesis of virion capsid proteins, but their data did indicate some identity between the tryptic[r] ...
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Attempts to convert the cellular prion protein into the scrapie isoform in cell-free systems.
... Incubation of MHM2 PrPC with PrP 27-30 in vitro under various conditions for 24 h Formation of: Incubation conditions PrPsc PrPc Additional treatment MoPrP 27-30 MoPrP 27-30 MoPrP 27-30 [r] ...
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Mechanism of premature polypetide termination in a mouse ascites cell-free system programmed by encephalomyocarditis viral RNA.
... In contrast to the inefficient release of viral RNAspecified polypeptides in other cell-free systems Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The premature termi[r] ...
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Early and late functions in a bipartite RNA virus: evidence for translational control by competition between viral mRNAs.
... Further experiments revealed that the overall concentration of exogenous viral mRNA added to the mRNA-dependent Drosophila cell-free systems had a greater influence on the relative propo[r] ...
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Inhibition by sulfonamides of the candidacidal activity of human neutrophils
... in cell-free systems containing purified human myeloperoxidase, hydrogen peroxide, and potassium iodide; and (c) accumulation of molecular iodine in analogously constructed cell- free ...
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An in vitro Biomolecular Breadboard for Prototyping Synthetic Biological Circuits
... original cell-free protocol, namely in process optimization, lysis methods, and energy ...power cell-free systems, such as pyruvate (Jewett & Swartz 2004), phosphoenolpyruvate ...
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Synthesis of Vaccinia Viral Proteins in Cytoplasmic Extracts
... in Incorporation of Radioactively Labeled Amino Acids into Polypeptides Received for publication 25 June 1969 Polypeptide synthesis has been studied in cell-free systems prepared from va[r] ...
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Stögbauer, Tobias Roland (2012): Experiment and quantitative modeling of cell-free gene expression dynamics. Dissertation, LMU München: Fakultät für Physik
... Cell-free systems were originally developed as a convenient means to synthesize proteins that are hard to produce in cells like toxic ...particular cell-free system which is generally ...
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Computational Programming for Product Designing in Synthetic Biology
... reprogram cell fate [2, ...in systems biology ...of cell-free systems to test model predictions under simplified and minimal ...
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On the Uplink Max-Min SINR of Cell-Free Massive MIMO Systems
... [4]–[9]. Cell-free Massive MIMO is a combination of distributed MIMO and Massive MIMO, and there is no cell boundary ...[4]. Cell-free Massive MIMO is thus also a scalable version of ...
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Cell-free expression systems for the construction of artificial cells
... In synthetic biology, a considerable defect of artificial cells (bottom-up approach) is the lack of a mechanisms for self-sustainment that is instead present in engineered living cells (top-down approach). Besides few ...
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Site directed labelling of proteins for NMR and EPR studies
... required the thiol groups of the protein to be activated with Ellman’s reagent (5,5- dithiobis-(2-nitrobenzoic acid), DTNB) before adding the DPA compounds to the protein. To overcome this cumbersome reaction step and ...
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