chemiluminescence enzyme immunoassay (CLEIA)
Development of a highly sensitive and selective microplate chemiluminescence enzyme immunoassay for the determination of free thyroxine in human serum
... microplate chemiluminescence enzyme immunoassay (CLEIA) with high sensitivity, selectivity and repro- ducibility was developed for the determination of free thyroxine (FT4) in human ...the ...
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Highly Sensitive Detection of Hepatitis B Virus Surface Antigen by Use of a Semiautomated Immune Complex Transfer Chemiluminescence Enzyme Immunoassay
... transfer chemiluminescence enzyme immunoassay (ICT-CLEIA) for the detection of HBsAg, which is as sensitive as hepatitis B virus (HBV) DNA PCR, was developed; the ICT- CLEIA assay ...
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Factors Influencing Serum Amyloid Type A (Saa) Concentrations in Horses
... Forsberg, “A Non-Competitive Chemiluminescence Enzyme Immunoassay for the Equine Acute Phase Protein Serum Amyloid A SAA—A Clinically Useful Inflammatory Marker in the Horse,” Veterinary[r] ...
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Detection of poliovirus antigen by enzyme immunoassay
... In conclusion, owing to the small amount of poliovirus in feces, the EIA is not suitable for the diagnosis of poliovirus infections directly from clinical specimens, but it can be used t[r] ...
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Clinical Drug Testing in Primary Care - Part 2
... An immunoassay based on competition for antibody binding sites between drug in the specimen and drug labeled with an ...enzyme. Enzyme activity decreases on binding to the antibody, so the drug when ...
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Solid phase enzyme immunoassay for Chlamydial antibodies
... Changes in chlamydial antibodies in paired sera of patients as determined by the EIA and I-IFAT testsa Patient Diagnosis Serum Days after EIA I-IFAT no.. NGU, Nongonococcal urethritis.[r] ...
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Clinical Evaluation of a Chemiluminescence Immunoassay for Determination of Immunoglobulin G Avidity to Human Cytomegalovirus
... The Liaison CMV IgG avidity assay evaluated here does not require sample dilution and is completed in 50 min. In the first incubation step, test sera are reacted with HCMV antigen coated onto magnetic particles in two ...
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The Applications of Gold Nanoparticle Initialed Chemiluminescence in Biomedical Detection
... with chemiluminescence technique in biological agents ’ ...both chemiluminescence and gold nanoparticles and conclude the different applications of gold nanoparticle-initialed chemiluminescence in ...
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Development of a highly specific and sensitive rubella immunoglobulin M antibody capture enzyme immunoassay that uses enzyme labeled antigen
... Development of a Highly Specific and Sensitive Rubella Immunoglobulin M Antibody Capture Enzyme Immunoassay That Uses Enzyme-Labeled Antigen HELENA SEPPANEN Labsystems Research Laborator[r] ...
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Detection of Histoplasma Antigen by a Quantitative Enzyme Immunoassay
... Antigen detection is a useful method for the diagnosis of histoplasmosis (8). Until now, antigen results have been ex- pressed semiquantitatively as antigen units (calculated as en- zyme immunoassay [EIA] units ...
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Enzyme immunoassay for the detection of group A streptococcal antigen
... At an optimal cutoff value of 40% inhibition, sensitivity and specificity by enzyme immunoassay were 97.0% and 97.9%, respectively, as compared with confirmed culture results.. The assay[r] ...
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Direct measurement of proinsulin in human plasma by the use of an insulin degrading enzyme
... this enzyme rapidly degrades insulin and insulin-like polypeptides to nonimmunoassayable components, whereas proinsulin and proinsulin cleaved at position B 54,55 are not appreciably ...chromatography. ...
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PubMedCentral-PMC4790930.pdf
... Gaithersburg, Maryland). After incubation and washing, extravidin (Sigma, St. Louis, Mis- souri) was added to the wells and incubated, and then the reactions were developed with 3,3 0 ,5,5 0 -tetramethylbenzidine (TMB; ...
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CD8+ T-Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected Subjects
... We have developed a single-cell enzyme immunoassay (enzyme-linked immunospot assay) specific for gamma interferon (IFN- ␥ ) production stimulated by either autologous B-lymphoblastoid ce[r] ...
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Transfusion-transmitted malaria and bacterial infections in a malaria endemic region
... Deoxyribonucleic acid Enzyme immunoassay Enzyme linked immunosorbent assay Full blood count Good Clinical Practice Good Clinical Laboratory Practice Global Database on Blood Safety Histi[r] ...
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Evaluation of enzyme linked immunoassay for serological diagnosis of cysticercosis
... The diagnosis of cysticercosis is based on clinical, epidemi- ologic, and serologic findings. MRI and/or computed axial tomographic scans are the most sensitive and specific diagnos- tic tools (9). However, because of ...
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Rapid Detection of Chlamydia pneumoniae by PCR Enzyme Immunoassay
... Chlamydia pneumoniae is an important human respiratory pathogen. Laboratory diagnosis of infection with this organism is difficult. To facilitate the detection of C. pneumoniae by PCR, an enzyme immunoassay ...
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Diagnostic Performance of C6 Enzyme Immunoassay for Lyme Arthritis
... C6 enzyme immunoassay (EIA) is a US Food and Drug Administration–cleared first-tier Lyme disease test with higher speci fi city compared with the older generation whole-cell sonicate EIA test and a test ...
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Solid phase enzyme immunoassay for determination of antibodies to cytomegalovirus
... A simple EIA for the determination of specific IgM antibodies by IIF, estimation of RF activity in sera, as well as a radioimmunoassay, and EIA methods presents method for the eliminatio[r] ...
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A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers
... To evaluate the immunoassay protocol used in this paper, a comparison with a reference method was car- ried out. Stress hormone is usually tested with a trad- itional ELISA assay which is performed in the majority ...
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