Chimeric fiber
Increased in vitro and in vivo gene transfer by adenovirus vectors containing chimeric fiber proteins.
... Recombinant vectors containing the fiber chimeras were efficiently produced by using an E4 rescue technique (Fig. 2). An E4-deleted vector, AdZ.11A, containing the lacZ gene driven by the CMV promoter was used to ...
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The Arg279Glu Substitution in the Adenovirus Type 11p (Ad11p) Fiber Knob Abolishes EDTA-Resistant Binding to A549 and CHO-CD46 Cells, Converting the Phenotype to That of Ad7p
... the fiber knobs of Ad11p and Ad7p were amplified by PCR with the following primers (DNA Technology A/S, Aarhus, Denmark), preserving the last shaft motif and the trimerization signal (13, 14): fk 11/7p forward (5 ...
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Selective targeting of human cells by a chimeric adenovirus vector containing a modified fiber protein.
... Ad5 fiber attachment receptor on primary human cells is poorly characterized, largely due to the fact that its identity is ...Ad5 fiber receptor. As noted prev- iously, the Ad3 fiber receptor, while ...
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Immunogenicity of Recombinant Fiber-Chimeric Adenovirus Serotype 35 Vector-Based Vaccines in Mice and Rhesus Monkeys
... capsid chimeric rAd35k5 ...capsid chimeric rAd35 vectors containing the Ad5 fiber knob ...Ad35 fiber gene with a chimeric fiber gene consisting of the Ad35 fiber tail and ...
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Fiber Shaft-Chimeric Adenovirus Vectors Lacking the KKTK Motif Efficiently Infect Liver Cells In Vivo
... the fiber shaft domains of both Ad31 and Ad41; therefore, we hypothesized that by analyzing the properties of fiber shaft-chimeric vectors, the role of the KKTK motif in observed virus infectivity in ...
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Generation of recombinant adenovirus vectors with modified fibers for altering viral tropism.
... full-length fiber ORF followed by part of the 39 untrans- lated region of the ...of fiber fusions, a unique EcoICRI restriction site was introduced at the 3 9 end of the fiber ORF by PCR-based ...the ...
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Genetic Linkage Map and QTL Analysis of Agronomic and Fiber Quality Traits in an Intraspecific Population
... This is the second map developed from an intraspecific population and uses the same set of cDNA probes developed from the same cDNA library. The present map shares 16 RFLP loci with that of Shappley et al. (1998a), plus ...
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The Large Polymerase Protein Is Associated with the Virulence of Newcastle Disease Virus
... the chimeric viruses ...the chimeric viruses, indicating that the F and HN genes and their homotypic interactions are not the major de- terminants of NDV virulence ...
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A Novel Self-Replicating Chimeric Lentivirus-Like Particle
... The chimeric virus vaccine particles produced after expression of unmodified Gag and Env from VEE RNA were morphologically similar to immature lentivirus particles, and Gag was incorpo- rated into them as the ...
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Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies
... A large number of different recombinant antigens have been produced in the Escherichia coli expression system. However, it is known that recombinant proteins produced in E. coli often loses their antigenic value due to ...
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Expression of human immunodeficiency virus type 1 (HIV-1) gag, pol, and env proteins from chimeric HIV-1-poliovirus minireplicons.
... A pulse-chase analysis of the protein expression from the chimeric genomes demonstrated the initial rapid proteolytic processing of the polyprotein from the chimeric genomes to give HIV-[r] ...
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Cryo electron Microscopy Structures of Chimeric Hemagglutinin Displayed on a Universal Influenza Vaccine Candidate
... Chimeric HA shows structural differences compared to H1 and H5 HA. Viruses displaying either H1 (pH1N1), H5 (H5N1), or cH5/1 (cH5/1N1) HA (Fig. 1A) were analyzed using cryo-electron FIG 1 Cryo-EM density maps ...
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Reduction of Natural Adenovirus Tropism to the Liver by both Ablation of Fiber-Coxsackievirus and Adenovirus Receptor Interaction and Use of Replaceable Short Fiber
... adenovirus fiber knob protein and its receptor, the coxsackievirus and adenovirus receptor ...short fiber is unable to recognize CAR, while the long fiber binds ...with chimeric Ad40-derived ...
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Car Drivers and Fuel Sources: How Distinct Signaling Domains in Chimeric Antigen Receptors Reprogram T Cells
... With breakthroughs in synthetic biology, improved cell culture techniques and advanced genetic engineering, it has now become possible to generate bi-specific primary human T cells with desired specificities. One mode of ...
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Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1
... five chimeric EN endonucleases (M-EN, T- EN, EN-NM, EN-M and EN-T) for pTR80 were 21-45- fold higher than that of TRAS1EN alone (Figure 3j, Addi- tional file ...
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Cloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium
... Kubota et al (2005) cloned cyt-P450 to construct a chimeric protein. They inserted this chimeric gene into pRED vector and expressed in into E. Their results show that this c[r] ...
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Understanding Zika Virus Stability and Developing a Chimeric Vaccine through Functional Analysis
... two chimeric viruses by swapping the prM-E genes between DENV-2 and ZIKV in the context of full-length cDNA ...prM-E chimeric viruses were 3- to 6-fold higher than those of the FIG 4 Comparison of levels of ...
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Genetic engineering of grass cell wall polysaccharides for biorefining
... Although most in planta CWD enzyme expression studies have assessed the effect of a single gene encoding for single enzyme activity, complete depolymerisation of lignocellulose requires a suite of CWD enzymes including ...
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Abstract: Despite advances in the understanding of its molecular pathophysiology, prostate cancer
... a chimeric antigen receptor (CAR) specific for prostate specific membrane antigen (PSMA), a glycoprotein that is overexpressed in prostate cancer, which expression involves neovasculature of several tumor ...
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Superinfection Exclusion between Two High-Risk Human Papillomavirus Types during a Coinfection
... In this study, we use native HPV16 and HPV18 virions, produced in the organotypic raft culture system, to examine the ability of single cells to be coinfected with more than one HPV type. Additionally, we investigate ...
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