CHO-K1
N- and 6-O-Sulfated Heparan Sulfates Mediate Internalization of Coxsackievirus B3 Variant PD into CHO-K1 Cells
... Increase in endosomal pH affects viral infection, but low pH does not induce instability of CVB3 PD. CVB3 PD can utilize CAR as well as HS to enter into host cells. To examine whether CVB3 PD infection depends on a low ...
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Transfecting CHO-K1 Cells: Comparison of CaPO4, Electroporation and Lipoplex Method with In-house Prepared Polyplex
... assay. CHO-K1 cell line was procured from National Centre For Cell Science (NCCS), Pune, India and maintained in DMEM medium containing 10% FBS at 37° in 5% CO 2 .... CHO-K1 cells were seeded ...
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A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A and alphaviruses fails to cleave Sindbis virus glycoprotein PE2.
... DISCUSSION In this article we report that RPE.40, a mutant strain of CHO-Kl cells selected for resistance to Pseudomonas exotoxin A and cross-resistant to alphaviruses 21, fails to cleav[r] ...
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Introduction of human genomic sequences renders CHO-K1 cells susceptible to infection by amphotropic retroviruses.
... The 1-gal titer of this ecotropic vector was similar to that of the PA317/LBgSN-conditioned medium when tested on NIH Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest To [r] ...
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The Murine Homolog (Mph) of Human Herpesvirus Entry Protein B (HveB) Mediates Entry of Pseudorabies Virus but Not Herpes Simplex Virus Types 1 and 2
... control CHO-K1 to serial dilutions of virus (data not shown) and as illustrated in ...periment, CHO-K1 cells were transiently transfected with the mHveB-expressing plasmid or a control plasmid ...
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Soluble V Domain of Nectin-1/HveC Enables Entry of Herpes Simplex Virus Type 1 (HSV-1) into HSV-Resistant Cells by Binding to Viral Glycoprotein D
... CHO-K1 cells. To measure HSV entry into cells, we used a replication-defective recombinant virus, QOZHG, that carries an expression cassette for  -galactosidase (6). This virus was chosen to prevent viral ...
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4919.pdf
... – CHO-K1, HeLa, and sympathetic neurons - by using the amphipathic peptide PEP-1 as a carrier (Figure ...into CHO-K1 and HeLa cells (Figure ...with CHO-K1 or HeLa ...
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Cellular Transcription Factor ZASC1 Regulates Murine Leukemia Virus Transcription
... (A) CHO-K1 or IM1 cells were challenged with the VSV-G- pseudotyped MLV vector MMP-nls-LacZ, which drives LacZ expres- sion from the MLV-LTR promoter, or pQCLIN, a self-inactivating vector with defective ...
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Enhanced optical manipulation of cells using anti reflection coated microparticles
... adherent CHO-K1 and NMuMG cells as well as the non-adherent HL60 cells, were considerably lower than those obtained for the either of the native particle ...
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Coexpression of ATP binding cassette proteins ABCG5 and ABCG8 permits their transport to the apical surface
... transfected CHO-K1 cells are consistent with the cova- lent addition of one N-linked sugar to ABCG8-HA and two N-linked sugars to ABCG5-myc (Figure ...
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Herpes B Virus Utilizes Human Nectin-1 but Not HVEM or PILRα for Cell-Cell Fusion and Virus Entry
... target CHO K1 cells with a plasmid encoding luciferase under a T7 promoter and either empty vector, nectin-1, AGM HVEM, or human ...Effector CHO-K1 cells were transfected with a plasmid ...
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Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador
... Methods: Nine different varieties of horchata were prepared in the traditional way and then freeze-dried. Phytochemical screening tested for the presence of secondary metabolites using standard procedures and antioxidant ...
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Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion
... transfected CHO-K1 cells was examined using Western blot analysis with a mouse monoclonal antibody (MAb) against the N-terminal FLAG epitope appended to Ov8; under reducing (treatment with a reducing buffer ...
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Expression and Characterization of a Minimal Hepatitis C Virus Glycoprotein E2 Core Domain That Retains CD81 Binding
... containing the mutation L441M to interact with full-length CD81 transfected into CHO-K1 cells. The data shown are the mean percentages of the levels of binding relative to the level of binding of the wild ...
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Investigation of triamterene as an inhibitor of the TGR5 receptor: identification in cells and animals
... Results: Triamterene dose dependently inhibits the increase in glucose uptake induced by TGR5 agonists in CHO-K1 cells expressing the TGR5 gene. In cultured NCI-H716 cells, TGR5 activation also increases ...
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Investigation of Clade B New World Arenavirus Tropism by Using Chimeric GP1 Proteins
... GP1 immunoadhesin binding activity. The lack of function- ality of the T(J) or A(G) GP chimeras containing full or partial substitutions of segment 4 frustrated our ability to further characterize receptor binding ...
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Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells
... 7 days on the LPT surface. The hUC-MSCs had a lower proliferation rate and better results on the Ti control surface (Fig. 5). The cells did not reach confluence and were distributed in multiple layers inside the porous ...
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Establishment a CHO Cell Line Expressing Human CD52 Molecule
... CHO-K1 cells were passaged 1 to 2 days before the electroporation, maintaining the cell density of about 75–85% confluency on the day of the experiment. Cells should be on passage 3-7. Before ...
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Stein, Bernhard (2004): Entwicklung von Potentialsensoren für die Zell-Halbleiter-Kopplung. Dissertation, LMU München: Fakultät für Physik
... Zunächst wurden CHO-K1-Zellen direkt auf dem Sensor-Chip kultiviert, und mit ei- nem Mikroskop überprüft ob diese Zellen gut adhärierten. Dazu wurde die Sensoro- berfläche gereinigt 8 und nicht weiter mit ...
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Development of betulinic acid as an agonist of TGR5 receptor using a new in vitro assay
... Results: Betulinic acid dose-dependently increases glucose uptake by CHO-K1 cells transfected with TGR5 only, which can be considered an alternative method instead of radioligand binding assay. ...
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