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Cloning, Molecular

MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE

MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE

... Molecular cloning refers to the procedure of isolating a defined DNA sequence and obtaining multiple copies of it in ...the cloning of PCR ...

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Molecular cloning and partial sequencing of hepatitis A viral cDNA.

Molecular cloning and partial sequencing of hepatitis A viral cDNA.

... Molecular cloning of foot and mouth disease virus genome and Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest the cloned sequences were HAV in origin, not from other seq[r] ...

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Chondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic Analysis

Chondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic Analysis

... DOI: 10.4236/ojms.2018.81007 138 Open Journal of Marine Science still limited; however, there are some reports on the occurrence of chitinase within the bodies of several varieties of sharks and stingrays [13], as well ...

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Molecular cloning and characterization of a thioredoxin from Taiwanofungus camphorata

Molecular cloning and characterization of a thioredoxin from Taiwanofungus camphorata

... Figure 3 Expression and purification of recombinant TcTrx in Saccharomyces cerevisiae. Fifteen μ L (loading buffer without mercaptoethanol and without boiling) of each fraction was loaded into each lane of the 12% ...

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Molecular cloning and nucleotide sequence of deer papillomavirus.

Molecular cloning and nucleotide sequence of deer papillomavirus.

... Furthermore, the 3' half of the BPV-1 El ORF exhibits striking homologies to nucleotide-binding domains of the simian virus 40 large T antigen 3, which suggests that the gene products of[r] ...

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In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment

<p>In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment</p>

... Prior to the construction of recombinant protein vac- cines, as opposed to CRC for clinical function, extensive testing is necessary. Recombinant protein vaccines must be harmless, stable in various conditions, have high ...

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Cloning and molecular characterisation of the murine 'c-ret' locus

Cloning and molecular characterisation of the murine 'c-ret' locus

... The molecular basis o f this process remains largely unresolved (see below); however the differential partitioning of morphogenic determinants of the germplasm, ...

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Molecular Cloning and Expression Analysis of PgLAC in Pomegranate

Molecular Cloning and Expression Analysis of PgLAC in Pomegranate

... of Molecular Biology plum and other species, degenerate primers were designed to amplify the inter- mediate fragment with a length of 449 bp (Figure ...The molecular weight was predicted to be ...

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The molecular cloning and characterization of the wheat Adh-1A gene

The molecular cloning and characterization of the wheat Adh-1A gene

... The evolutionary relationships between the diploid and polyploid wheats have been determined mainly by cytology, chromosome pairing studies and more recently using molecular probes to highly repeated DNA ...

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Molecular cloning and physical mapping of the tupaia herpesvirus genome.

Molecular cloning and physical mapping of the tupaia herpesvirus genome.

... Restriction maps for the restriction endonucleases EcoRI and Hindlll were constructed with data derived from Southern blot hybridizations of individual viral DNA fragments or cloned DNA [r] ...

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Molecular cloning and physical mapping of murine cytomegalovirus DNA.

Molecular cloning and physical mapping of murine cytomegalovirus DNA.

... The results obtained after hybridization of 32P-labeled cloned fragments to Southern blots of MCMV DNA cleaved with HindIII, XbaI, EcoRI, BamHI, ApaI, ClaI, EcoRV, or KpnI allowed us to [r] ...

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Identification, Molecular Cloning, and Analysis of Full Length Hepatitis C Virus Transmitted/Founder Genotypes 1, 3, and 4

Identification, Molecular Cloning, and Analysis of Full Length Hepatitis C Virus Transmitted/Founder Genotypes 1, 3, and 4

... T/F molecular clones were used to generate vRNA for studies of innate immune signal- ing, with findings reported in the accompanying article (88) and elsewhere ...replication-competent molecular clone JFH-1 ...

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Molecular cloning of an enhancer binding protein.pdf

Molecular cloning of an enhancer binding protein.pdf

... From a series of screens of protein replica filters generated from platings of the ~,EB phage, the following conclusions were drawn: the specific detection of ZEB plaques requ[r] ...

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Molecular cloning, sequencing, and expression of Eimeria tenella HSP70 partial gene.

Molecular cloning, sequencing, and expression of Eimeria tenella HSP70 partial gene.

... ABSTRACT. Members of the Eimeria genus are protozoan parasites of the subphylum Apicomplexa (Eimeriidae family), and belong to the coccidia group. Eimeria tenella is one of the most pathogenic species owing to its ...

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Molecular cloning and differential expression of the glucocorticoid receptorgene in the estuarine tapertail anchovy Coilia nasus.

Molecular cloning and differential expression of the glucocorticoid receptorgene in the estuarine tapertail anchovy Coilia nasus.

... Unlike the non-NaCl group, plasma osmolality increased significantly in the NaCl group compared with the control fish, but was maintained at a level similar to the control level after [r] ...

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Molecular cloning of Peking duck Toll like receptor 3 (duTLR3) gene and its responses to reovirus infection

Molecular cloning of Peking duck Toll like receptor 3 (duTLR3) gene and its responses to reovirus infection

... The innate immune system is a major contributor to acute inflammation induced by microbial infection or tissue damage. The initial sensing of infection is mediated by in- nate pattern recognition receptors (PRRs), which ...

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Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaechei

Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaechei

... Results: Six TreS producing strains were isolated from a specimen obtained from soil of the Tibetan Plateau using degenerate PCR. A novel treS gene from Enterobacter hormaechei was amplified using thermal asymmetric ...

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Bioinformatics analysis and molecular cloning of an extracellular serine protease from acinetobacter baumannii

Bioinformatics analysis and molecular cloning of an extracellular serine protease from acinetobacter baumannii

... Sievers, F., Wilm, A., Dineen, D., Gibson, T.J., Karplus, K., Li, W., Lopez, R., McWilliam, H., Remmert, M., Söding, J. and Thompson, J.D. (2011). Fast, Scalable Generation of High-quality Protein Multiple Sequence ...

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Isolation and characterization of a novel anionic peroxidase cDNA found in poplar (Populus nigra) suspension cultured cells

Isolation and characterization of a novel anionic peroxidase cDNA found in poplar (Populus nigra) suspension cultured cells

... Lagrimini LM, Burkhart W, Moyer M, Rothstein S (1987) Molecular cloning of complementary DNA encoding the lignin- forming peroxidase from tobacco: molecular analysi[r] ...

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Ligand-Gated Ion Channels in Sensory Neurons

Ligand-Gated Ion Channels in Sensory Neurons

... Molecular Cloning of a Human Neuronal Nicotinic Acetycholine Receptor p3-like Subunit. Molecular Cloning of a Resiniferatoxin-binding Protein.[r] ...

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