Cobas Amplicor M. tuberculosis™ kit
Comparison of In house PCR with Conventional Techniques and Cobas Amplicor M tuberculosis™ Kit for Detection of Mycobacterium tuberculosis
... sensitivities for AFS, culture, and PCR were 49.2%, 80.7%, 77.5%, respectively, and specificities were 100%, 100%, and 99.7%, respectively, in- dicating lower sensitivity and higher specificity. Another study ...
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Comparison of Diagnostic Performance of Three Real Time PCR Kits for Detecting Mycobacterium Species
... the COBAS Amplicor MTB, the CO- BAS TaqMan MTB, the AdvanSure TB/NTM real-time PCR, and the Real-Q ...Mycobacterium tuberculosis (MTB) detection, all five kits showed 100% matching results (positive; ...
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Direct Detection of Rifampin Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR DNA Sequencing
... (Cobas Amplicor Diagnostics); 0.4 M (each) primers (Life Technologies), and 2 U of AmpliTaq Gold Polymerase (Applied ...reaction kit (version ...
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Comparison of Roche Cobas Amplicor Mycobacterium tuberculosis Assay with In House PCR and Culture for Detection of M tuberculosis
... Taq polymerase (Pharmacia, Freiburg, Germany) per 50 ml, 1 U of UNG (Boehr- inger Mannheim, Mannheim, Germany) per 50 ml, 200 mM dGTP, 200 mM dATP, 200 mM dCTP, 600 mM dUTP, and 0.5 mM each primer (final concen- ...
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Systematic Review of the Performance of HIV Viral Load Technologies on Plasma Samples
... Methods and Findings: A search of Medline and Embase was conducted for studies evaluating the accuracy or reproducibility of commercially available HIV VL assays. 37 studies were included for review including evaluations ...
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Dynamic Range of Hepatitis C Virus RNA Quantification with the Cobas Ampliprep Cobas Amplicor HCV Monitor v2 0 Assay
... rectly when below 500,000 IU/ml (3). The results of our study show that the actual upper limit of quantification of the semi- automated Cobas version of this assay following automated extraction with Cobas ...
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Determination of Chlamydia trachomatisPrevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens
... The urine specimens in our study are mailed specimens that were obtained in the home. This approach has been reported before (1, 21) and has been validated with respect to DNA degradation and reliable C. trachomatis DNA ...
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Performance of the Amplicor human immunodeficiency virus type 1 PCR and analysis of specimens with false negative results
... was for confirmation of infection. Serological diagnosis of HIV infection was performed according to the recommendations of the AIDS Diagnosis Working Group of the Public Health Laboratory Service (26) and the World ...
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Multicenter Evaluation of the AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Detection ofChlamydia trachomatis
... automated COBAS AMPLICOR CT/NG and semiautomated AMPLICOR CT/NG tests were evaluated in a multicenter trial for the ability to detect Chlamydia trachomatis ...The COBAS AMPLICOR and ...
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Evaluation of conventional and molecular strategies for the rapid diagnosis and molecular characterisation of strains of Mycobacterium tuberculosis
... Comparativestudy of Amplicor for ligase direct detection chain reaction and reaction of polymerasechain M tuberculosis in clinical specimens.. Saudi Medical Journal.[r] ...
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Impact of Proficiency Testing Program for Laboratories Conducting Early Diagnosis of HIV 1 Infection in Infants in Low to Middle Income Countries
... The upper limit on the total discordant results was generally less than 2.5% for any testing cycle with the exception of the very first PT panel; there was a higher rate of false-negative results at the 2006-2 cycle than ...
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Evaluation of the COBAS TaqMan HCV Test with Automated Sample Processing Using the MagNA Pure LC Instrument
... open COBAS TaqMan kinetic reaction tubes (K-tubes) contained in a K-tube holder (K-carrier) were placed onto a prototype (noncooling) postelution handling block specifically designed to hold a K-carrier ...
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Accelerated Detection and Identification of Mycobacteria with MGIT 960 and COBAS AMPLICOR Systems
... The MGIT system has several advantages over the wide- ly used, semiautomated BACTEC 460 system, including de- creased manual labor requirements, no radioactive waste, and no need to use a needle and syringe for ...
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Multicenter Evaluation of the Fully Automated COBAS AMPLICOR PCR Test for Detection of Chlamydia trachomatis in Urogenital Specimens
... Use of the IC increased the test sensitivity for urine and swab specimens from women because two initially inhibitory specimens were found to be true positive upon retesting. Nu- merous studies have demonstrated that a ...
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Evaluation of the Abbott RealTime CT/NG Assay in Comparison to the Roche Cobas Amplicor CT/NG Assay
... Roche Cobas Amplicor CT/NG assay used for clinical testing in our hospital system and the Abbott RealTime CT/NG ...(T m ) of the hybridization ...
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Neisseria Species Identification Assay for the Confirmation of Neisseria gonorrhoeae Positive Results of the COBAS Amplicor PCR
... concentration), 0.2 mM NG767F16 primer, 1.0 mM NG964R16 primer, 0.2 mM NG839GR3 probe, and 0.4 mM NG2RED primer. Increased sensitivity was observed when the master mix was predigested with HpaII restriction enzyme (1 U ...
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Evaluation of Conventional and Real Time PCR Assays Using Two Targets for Confirmation of Results of the COBAS AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae Test for Detection of Neisseria gonorrhoeae in Clinical Samples
... dCTP, dGTP, and dUTP), 1.5 U AmpliTaq Gold polymerase, and 0.25 g of each primer. Amplification parameters for the 16S rRNA PCR were as follows: an initial denaturation at 95°C for 5 min; 40 cycles of denaturation at ...
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Comparative Evaluation of PCR with Commercial Multiplex M tuberculosis Detection Kit
... On the other side, the PCR positivity was found 100% in smear positive samples by utilizing In House PCR as well as commercial multiplex M. tuberculosis PCR kit. One culture positive & smear ...
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Clinical Evaluation of the Automated COBAS AMPLICOR MTB Assay for Testing Respiratory and Nonrespiratory Specimens
... the COBAS AMPLICOR PCR system (Roche Diagnostics) for the routine detection of Mycobacterium tuberculosis complex (MTBC) in clinical ...were COBAS AMPLICOR MTB positive, and of the ...
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Correction of Underquantification of Human Immunodeficiency Virus Type 1 Load with the Second Version of the Roche Cobas AmpliPrep/Cobas TaqMan Assay
... the Cobas AmpliPrep/Cobas TaqMan human immunodeficiency virus type 1 (HIV-1) test (CAP/CTM) demonstrated good performance but, afterwards, reports about underquantification were ...the Cobas ...
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