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Confocal laser scanning microscopy and image analysis

Z-Directional distribution of fiber orientation of Japanese and western papers determined by confocal laser scanning microscopy

Z-Directional distribution of fiber orientation of Japanese and western papers determined by confocal laser scanning microscopy

... Regardless of the sheet-forming tool (machine or hand), fi ber orientation is one of the most important factors of paper properties and its distribution in the thickness direc- tion plays an important roll for ...

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LASER SCANNING CONFOCAL MICROSCOPY

LASER SCANNING CONFOCAL MICROSCOPY

... Abstract Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living ...

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CONFOCAL LASER SCANNING MICROSCOPY TUTORIAL

CONFOCAL LASER SCANNING MICROSCOPY TUTORIAL

... On Our Microscopes: The tables below can be used to set up Nyquist sampling on the Zeiss LSM 10 Pascal and the Olympus FV500. In these tables, the value r xy is the calculated resolution of the lens in x and y based on a ...

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Correlative analysis of immunoreactivity in confocal laser-scanning microscopy and scanning electron microscopy with focused ion beam milling

Correlative analysis of immunoreactivity in confocal laser-scanning microscopy and scanning electron microscopy with focused ion beam milling

... Tissue information was obtained only by milling the tissue by using the FIB-SEM technique. When the region of interest was located in the center of the sample, at least one part of the sample was damaged in order to ...

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The use of confocal laser scanning microscopy for the study of dentin infection

The use of confocal laser scanning microscopy for the study of dentin infection

... of confocal microscopy is the ability to analyze by thin optical sections the subsurface structure of biological ...the confocal microscopy to investigate the pattern of dentin infection ...

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In vivo evaluation of DSAEK interface with scanning-laser confocal microscopy

In vivo evaluation of DSAEK interface with scanning-laser confocal microscopy

... Similarly to others [17,18], we found no correlation between particle number and visual acuity. Small parti- cles located at the interface are a common finding fol- lowing DSAEK, and are also observed after LASIK surgery ...

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Cryo-laser scanning confocal microscopy of diffusible plant compounds

Cryo-laser scanning confocal microscopy of diffusible plant compounds

... The experimental setup presented here was developed to image metallic cations and phenolic compound distribu- tions in in vivo-like fleshy fruit by fluorescence confocal microscopy. Particular care ...

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Confocal laser scanning microscopy analysis of S  epidermidis biofilms exposed to farnesol, vancomycin and rifampicin

Confocal laser scanning microscopy analysis of S epidermidis biofilms exposed to farnesol, vancomycin and rifampicin

... antimicrobial therapy against S. epidermidis. However, its mechanism of action in S. epidermidis is not fully known. In this work we better elucidate the role of farnesol against S: epidermidis biofilms using ...

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Characterisation of solidification using combined confocal scanning laser microscopy with infrared thermography

Characterisation of solidification using combined confocal scanning laser microscopy with infrared thermography

... IRTs typically operate at one of two frequency ranges, either around 1-1.5 μm or 8-14 μm. The halogen bulb used as a heat source in this set up operates at a frequency 300-2000 nm, therefore any imaging source that is ...

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RESEARCH REPORT ON CONFOCAL LASER SCANNING MICROSCOPY STUDIES ON FURFURYLATED WOOD SAMPLES

RESEARCH REPORT ON CONFOCAL LASER SCANNING MICROSCOPY STUDIES ON FURFURYLATED WOOD SAMPLES

... a confocal laser scanning microscope, the laser beam diameter is limited by a pinhole aperture that is situated in a conjugate plane (confocal) with a scanning point on the ...

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Parametric Blind Deconvolution for Confocal Laser Scanning Microscopy (CLSM)-Proof of Concept

Parametric Blind Deconvolution for Confocal Laser Scanning Microscopy (CLSM)-Proof of Concept

... fluorescence Confocal Laser Scanning Microscope (CLSM) images with parametric estimation of the acquisition sys- tem’s Point Spread Function ...the image suffers from two primary physical ...

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Iris ultrastructure in patients with synechiae as revealed by in vivo laser scanning confocal microscopy

Iris ultrastructure in patients with synechiae as revealed by in vivo laser scanning confocal microscopy

... We found that the support structure of the iris branches gradually from coarse to fine. Based on the size of the support structure, the stent surface of iris can be divided into three classes of structures: tree trunk-, ...

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Tandem scanning confocal microscopy (TSCM), with its

Tandem scanning confocal microscopy (TSCM), with its

... 1995, Confocal microscopy through focusing (CMTF) was developed in our laboratory using a Unix graphic work- station (INDY; Silicon Graphics, Mountain View, CA) and has since been used for objectively ...

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Studies of the microstructure of polymer modified bitumen emulsions using confocal laser scanning microscopy

Studies of the microstructure of polymer modified bitumen emulsions using confocal laser scanning microscopy

... Polymer-modified bitumen emulsions present a safer and more environmentally friendly binder for enhancing the properties of roads. Cationic bitumen emulsion binders containing polymer latex were investigated using ...

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The neuromuscular system of Pycnophyes kielensis (Kinorhyncha: Allomalorhagida) investigated by confocal laser scanning microscopy

The neuromuscular system of Pycnophyes kielensis (Kinorhyncha: Allomalorhagida) investigated by confocal laser scanning microscopy

... a confocal laser scanning microscope of two kinorhynchs with introvert slightly ...transmission image to show the position of the muscles relative to the ...

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Histometric data obtained by in vivo confocal laser scanning microscopy in patients with systemic sclerosis

Histometric data obtained by in vivo confocal laser scanning microscopy in patients with systemic sclerosis

... Results: Micromorphologic characteristics of skin of patients with systemic sclerosis and measuring parameters for melanisation, epidermal hypotrophy, and fibrosis for dislocation of capillaries by collagen deposits in ...

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Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro

Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro

... corneum, confocal laser scanning micros- copy, tape stripping Background Nanotechnology, a rapidly emerging field, provides new techniques and ...

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Elucidating the pulsed-dye laser treatment of sebaceous hyperplasia in vivo with real-time confocal scanning laser microscopy

Elucidating the pulsed-dye laser treatment of sebaceous hyperplasia in vivo with real-time confocal scanning laser microscopy

... a laser beam on a specific point in the skin, detecting only the light reflected from the focal point through a pinhole- sized spatial ...to image successive horizontal planes stacked ...

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Extended Field Laser Confocal Microscopy (EFLCM): Combining automated Gigapixel image capture with in silicovirtual microscopy

Extended Field Laser Confocal Microscopy (EFLCM): Combining automated Gigapixel image capture with in silicovirtual microscopy

... one image consisting of all signals through the entire Z-axis is ...only confocal signals from the different focal planes can reach the ...automatic image subtractions using ...

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Chapter 13 Confocal Laser Scanning Microscopy C. Robert Bagnell, Jr., Ph.D., 2012

Chapter 13 Confocal Laser Scanning Microscopy C. Robert Bagnell, Jr., Ph.D., 2012

... Light Microscopy 8 of a PMT can be adjusted by electrical controls that represent brightness and contrast in the final ...the image and 255 represents white (or visa ...the laser beam sweeps along ...

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