• No results found

constant denaturing gradient gel electrophoresis (CDGE)

Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity
 

Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity  

... optimize gradient gel electrophoresis (DGGE) protocols for determination of marine eukaryotic phytoplankton genetic ...a constant 150V and a temperature of 60 o C in TAE ...

11

Diversity of Hepatitis C Virus Quasispecies Evaluated by Denaturing Gradient Gel Electrophoresis

Diversity of Hepatitis C Virus Quasispecies Evaluated by Denaturing Gradient Gel Electrophoresis

... The denaturing-gradient polyacrylamide gel was prepared as ...The gradient gel was formed by transferring, using a gravity-driven gradient maker (GRI, Braintree, United Kingdom), ...

12

Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles

Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles

... linear gradient of the denaturants urea and formamide, increasing from 40% at the top of the gel to 80% at the bottom (with 100% denaturants corresponding to 7 M urea and 40% [vol/vol] deionized ...a ...

8

A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations

A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

... developed by in 1987 [26]. DHPLC as opposed to DGGE does not require extensive 2-D gradient gel electrophoresis but knowledge about the predicted DNA melting temperature is required. The predicted ...

10

Persistence of orally administered Megasphaera elsdenii and Ruminococcus bromii in the rumen of beef cattle fed a high grain (barley) diet

Persistence of orally administered Megasphaera elsdenii and Ruminococcus bromii in the rumen of beef cattle fed a high grain (barley) diet

... The steers were fed for a total of 70 days with commercial feedlot diets incorporating starter (Days 1 – 6), intermediate (Days 7–12) and finishing (Days 13–70) rations (Table 1). The steers were fed once daily around ...

8

Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci

Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci

... Therefore, electrophoresis of amplified fragments provides band patterns specific for different bacterial ...by electrophoresis in 1.5% agarose gel, stained with ethidium bromide, and de- tected by ...

6

An investigation into the potential impacts of ocean acidification and ocean fertilisation on the genetic diversity of marine bacterial assemblages

An investigation into the potential impacts of ocean acidification and ocean fertilisation on the genetic diversity of marine bacterial assemblages

... There have been many advances in molecular techniques aimed at revealing the structure of in situ marine microbial communities. Denaturing gradient gel electrophoresis (DGGE), described in ...

277

Evidence of melanoma in wild marine fish populations

Evidence of melanoma in wild marine fish populations

... verified by agarose gel electrophoresis [1.6% (w/v) agarose] with ethidium bromide staining and visualized using a UV transillumi- nator. DGGE was performed using the D-Code universal mutation detection ...

7

Structural Shifts of Mucosa Associated Lactobacilli and Clostridium leptum Subgroup in Patients with Ulcerative Colitis

Structural Shifts of Mucosa Associated Lactobacilli and Clostridium leptum Subgroup in Patients with Ulcerative Colitis

... UC. Denaturing gradient gel electrophoresis analysis was employed to profile the composition of the dominant bacteria (16S rRNA gene V3 region) and three important groups: lactobacilli, the ...

5

Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia

Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia

... In a previous study [29], we reported the construction of two novel bacterial-fungal consortia involved in the bioconversion of lignocellulose next to furanic com- pounds. We described their characteristics based on ...

18

Elucidation of  The Microbial Community in Activated Sludge using PCR-DGGE Analysis in Arid and Semi Arid Regions of Rajasthan

Elucidation of The Microbial Community in Activated Sludge using PCR-DGGE Analysis in Arid and Semi Arid Regions of Rajasthan

... Activated sludge is the most commonly used process to treat sewage and industrial waste water by micro organisms. The activated sludge system depends on the activities of microbial communities present in the sludge. ...

11

PCR DGGE assessment of the bacterial diversity of breast milk in women with lactational infectious mastitis

PCR DGGE assessment of the bacterial diversity of breast milk in women with lactational infectious mastitis

... by gel excision and PCR sequencing are indi- cated by letters from (q) to (z) and from (?) to (?), and correspond to the following bacteria: q, Gemella haemolysans [EMBL: AM774574]; r, Acinetobacter ...

8

Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis

Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis

... PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting was first used for profiling complex microbial populations by Muyzer et ...

18

Genome of infectious bronchitis virus.

Genome of infectious bronchitis virus.

... Analysis on sucrose gradients under both aqueous and denaturing conditions together with agarose gel electrophoresis in the presence of the chaotropic agent methylmercuric hydroxide gave[r] ...

9

Erratum to Bacterial diversity in toxic Alexandrium tamarense blooms off the Orkney Isles and the Firth of Forth

Erratum to Bacterial diversity in toxic Alexandrium tamarense blooms off the Orkney Isles and the Firth of Forth

... Another significant group is bacteria of the Bac- teroidetes phylum, a group which is often thought to be involved in particle degradation (De Long et al. 1993; Cottrell and Kirchmann 2000a; Riemann et al. 2000; Eilers ...

11

Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers

Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers

... The bacterial microfloras of 8 healing and 10 nonhealing chronic venous leg ulcers were compared by using a combination of cultural analysis and denaturing gradient gel electrophoresis (DGGE) ...

9

Missing band 7 membrane protein in two patients with high Na, low K erythrocytes

Missing band 7 membrane protein in two patients with high Na, low K erythrocytes

... SDS gel electrophoresis in the first dimension combined with SDS gel electrophoresis in the second dimension) resolved the 28,000-dalton component from normal erythrocyte membranes into two ...

9

Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR

Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR

... amide gel under UV illumination. The gel fragments were washed once in 200 μl of sterile deionized water and kept in 50 μl of sterile water overnight at 4°C for ...extracted gel mix was heated at ...

7

High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing

High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing

... PCR-denaturing gradient gel electrophoresis and ...multiplex denaturing gradient gel electrophoresis, pyrosequenc- ing, and a ...

8

TP53 hotspot mutations are predictive of survival in primary central nervous system lymphoma patients treated with combination chemotherapy

TP53 hotspot mutations are predictive of survival in primary central nervous system lymphoma patients treated with combination chemotherapy

... DGGE: denaturing gradient gel electrophoresis; DLBCL: diffuse large b-cell lymphoma; FFPE: formalin fixed paraffin embedded; HD-MTX: high-dose methotrexate; HR: hazard ratio; IARC: ...

13

Show all 10000 documents...

Related subjects