crystallizing membrane proteins
Crystallizing membrane proteins for structure function studies using lipidic mesophases
... for crystallizing membrane proteins has posted some high-profile successes ...every membrane structural biology laboratory and that is ...of membrane protein types and ...the ...
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Crystallizing membrane proteins using lipidic mesophases
... In meso crystallization was performed with monoolein as the host lipid. The lipidic mesophase laden with protein was dispensed into home-made 96-well glass sandwich crystallization plates using a home-built in meso ...
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Crystallizing Membrane Proteins in Lipidic Mesophases A Host Lipid Screen
... outer membrane proteins crystallized by the in meso method, have been ...JBS Membrane HT screen conditions that included 25 %(v/v) tri-ethylene glycol, ...
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Crystallizing Membrane Proteins in the Lipidic Mesophase Experience with Human Prostaglandin E2 Synthase 1 and an Evolving Strategy
... 3.3. Initial Trials and Preliminary Optimization. 3.3.1. DOPC Was Critical for Obtaining Initial in Meso Crystals. Initial in meso crystallization trials were set up at 20 ° C with monoolein (9.9 MAG) as the host lipid ...
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Crystallizing membrane proteins for structure determination using lipidic mesophases
... chromophore-containing and chromophore-free, and alpha-helical and beta-barrel proteins. Recent successes using in meso crystallization are the human engineered beta2-adrenergic and adenosine A2a G protein-coupled ...
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A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes
... for crystallizing membrane proteins for structure ...anchored proteins are ...large proteins and complexes is ...soluble proteins should not be ...
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Structure, Function & Dynamics At The Membrane
... a membrane mimetic, cardiolipin was introduced to confirm two previously predicted sites of interaction as well as to identify and propose a novel third ...of membrane proteins remains to be ...
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The M18 aspartyl aminopeptidase of Plasmodium falciparum binds to human erythrocyte spectrin in vitro
... erythrocyte membrane skel- eton ...parasite proteins, for example, P. falciparum erythrocyte membrane protein 3 (PfEMP-3), mature parasite-infected surface antigen (MESA), falciparum-exported ...
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Comparison of outer membrane protein and biochemical profiles of Haemophilus aegyptius and Haemophilus influenzae biotype III
... influenzae biotype III had major outer membrane proteins between 27 and 41.5 kDa representing two additional protein profiles.... OUTER MEMBRANE PROTEINS OF HAEMOPHILUS SPP..[r] ...
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Membrane Stored Curvature Elastic Stress Modulates Recruitment of Maintenance Proteins PspA and Vipp1
... the membrane compared with use of lipid extracts, as both lipid head and tail groups can be precisely se- ...pronounced membrane interaction (see ...sole membrane-binding deter- ...of proteins ...
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Inflammasome Activation by Bacterial Outer Membrane Vesicles Requires Guanylate Binding Proteins
... the membrane-remodeling dynamin protein superfamily (35), GBPs could potentially play a role in controlling membrane dynamics at the OMV- endosomal interface and thereby expose the lipid A moiety of LPS ...
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Lu, Aiping (2008): Development of proteomics techniques for individual protein and membrane protein mapping. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... of proteins, including post-translational modifications, remains a challenging ...task. Proteins are usually digested to tryptic peptides that are measured either by MALDI peptide mapping or by liquid ...
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Adam, Julia (2016): Cryo-EM studies of tubular membrane shaping by the N-BAR protein amphiphysin. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... tubular membrane shapes and the three dimensional structure of the amphiphysin assembly were ...amphiphysin membrane interaction was analyzed by performing in vitro liposome tubulation assays with various ...
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Microbial expression systems for membrane proteins
... Folded membrane proteins in their native membranes can usually be solubilized with ...recombinant membrane proteins are difficult to ...target membrane protein at 1 mg/mL in three di ff ...
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Endosomal trafficking of yeast membrane proteins
... of membrane trafficking pathways in yeast have been achieved by: 1) observing steady state localisation of trafficking cargoes / compartment markers and 2) using genetic perturbations that accumulate trafficking ...
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Circular dichroism spectroscopy of membrane proteins
... a membrane protein is embedded is considerably lower than that of water ...of proteins measured in these environments compared to the spectra of proteins comprised of similar secondary structures but ...
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Signaling Activities of Gammaherpesvirus Membrane Proteins
... latent membrane protein 1 (LMP1) of EBV, K1 of KSHV, and R1 of ...These proteins do not share sequence relatedness, with the exception of limited structural homology between R1 and K1, which is reflective ...
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Mechanisms Of Membrane Remodeling By Bar Proteins
... reduced membrane binding capacity of H0 truncated ...to membrane and thus drive membrane ...domain’s membrane binding was compromised and failed to drive membrane curvature [5, ...of ...
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Genetic Similarity of Gonococcal Homologs to Meningococcal Outer Membrane Proteins of Serogroup B Vaccine
... outer membrane vesicles (OMVs) have been utilized in a number of countries, where they are primarily designed to match outbreak strains circulating during regional epidemics (17, ...
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A versatile strategy for production of membrane proteins with diverse topologies: application to investigation of bacterial homologues of human divalent metal ion and nucleoside transporters
... Membrane proteins play key roles in many biological processes, from acquisition of nutri- ents to neurotransmission, and are targets for more than 50% of current therapeutic ...recombinant membrane ...
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