Culture of epithelial stem cells
Anatomical location and culture of equine corneal epithelial stem cells
... to stem cells, to the meroclone and then to the ...the cells isolated from group L, which consisted of ELECs, correspond to the features of ...the cells iso- lated from the central cornea ...
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Comparative proteomics reveals human pluripotent stem cell-derived limbal epithelial stem cells are similar to native ocular surface epithelial cells
... Langerhans cells 38 ...hPSC-derived cells pos- sess a certain degree of immune privilege 40,41 , which would also explain the scarcity of identified immune response ...niche cells possess the ability ...
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An assessment of the differentiation potential of epithelial stem cells in adult endometrium
... to epithelial SPCs found in the skin, liver and ...that epithelial cells used by this group were passaged up to 15 times: it is possible cellular phenotype changed in culture within this time ...
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Epithelial stem cells of the lung: privileged few or opportunities for many?
... basal cells. One system involves seeding dissociated airway epithelial cells on to membranes and culturing them at an air- liquid ...tracheal cells isolated from Rosa26 mice (which ...
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Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
... of epithelial stem cells for regenerative medicine Despite promising strategies that use human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells for ...
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Efficacy of cultivated corneal epithelial stem cells for ocular surface reconstruction
... cell culture needed more time than the allograft, perhaps because the superficial limbal tissue sec- tions obtained from the contralateral normal eyes were somewhat smaller than the cadaveric limbal donors’ (their ...
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The characterisation of endometrial epithelial stem/progenitor cells
... endometrial epithelial cells. The primary endometrial epithelial cells sorted on the expression of SSEA-1 are expected to contain a SPC population; therefore we evaluate the expression of ...
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Interactions between rat alveolar epithelial cells and bone marrow-derived mesenchymal stem cells: an in vitro co-culture model
... these cells underwent phenotype changes as a result of culture conditions or interactions with ...co- culture model should be designed as the one in which BMSCs are seeded on the AEC monolayer on ...
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Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation
... current culture conditions are suboptimal for the expan- sion of these stemlike cells, and modest changes in for- mulation of culture medium can affect ...stromal stem cells within the ...
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Endothelial cells stimulate growth of normal and cancerous breast epithelial cells in 3D culture
... parenchymal cells such as the epithelial ...endothelial cells are important for fate control of stem cells, organogenesis and tissue ...
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Human conjunctival epithelial precursor cells and their progeny in 3D organotypic culture
... ”Stem Cells and Life”, Pisa and Institute of Biomedical Technologies, ...organ culture method to investigate human conjunctival epithelial basal precursor cells and their progeny within ...
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The in vitro differentiation of prostate epithelial cells and gonadal adipocytes from mouse embryonic stem cells
... The culture conditions described here were designed to induce hindgut endoderm as demonstrated in Chapter ...columnar epithelial cells with a central nucleus and connected by tight junctions (Grubb, ...
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Wnt signaling regulates trans-differentiation of stem cell like type 2 alveolar epithelial cells to type 1 epithelial cells
... lung epithelial cells were isolated from lung tissue samples of lobectomy patients with normal lung function, then were cultured on collagen-coated plastic ...of culture and real-time qPCR analysis ...
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Cyclin D1 affects epithelial–mesenchymal transition in epithelial ovarian cancer stem cell-like cells
... Epithelial cells have long been regarded as cells of terminal differentiation, but recent studies have found that epithelial cells can lose adhesion, tight junction, and cell polarity ...
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Generation of lung epithelial-like tissue from human embryonic stem cells
... embryonic stem cells (hESC) have the capacity to differentiate in vivo and in vitro into cells from all three germ ...specific culture conditions on the differentiation of hESC into lung ...
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Interfollicular epidermal stem-like cells for the recreation of the hair follicle epithelial compartment
... mesenchymal cells, human HF regeneration remains elu- ...immature epithelial cells, and of suitable culture conditions capable of minimizing cell differentiation in ...
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Epithelial Sodium Channels in Pulmonary Epithelial Progenitor and Stem Cells
... various cells and mechanisms contribute to this regeneration and ...Club cells, bronchioalveolar stem cells (BASCs), and alveolar type II epithelial cells (ATII) are dominant ...
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The Epithelial-Mesenchymal Transition Generates Cells with Properties of Stem Cells
... transformed cells that have undergone an EMT, we injected HMLEN cells that carried either Snail-ER or Twist-ER and had been treated with 4-OHT for 12 days prior to implantation in limiting dilutions into ...
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Culture and transplantation of spermatogonial stem cells
... testis cells. In addition, although we as- sumed that younger germ cells would be more sensitive to induction of pluripotency, in vitro transfection experiments revealed that PGCs de- rived from ...
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Gut instincts: thoughts on intestinal epithelial stem cells
... The stem-cell niche One major unresolved issue in stem-cell biology is whether stem cells are intrinsically different from their dividing cells or whether they are instructed by their ...
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