Culture Studies

Various concentrations of turmeric were used along with MS media under similar environmental conditions. The percentage of contamination observed is represented in table 4 and figure 1. MS media with 0.8 g/L and 1.0 g/L of turmeric and 2.0 g/L of carbendazium has recorded nil contamination, while highest contamination was recorded using MS media with K. cyclin (0.5 and 1.0 g/L concentration) and MS media alone (Figure 2). The contamination was observed even with the turmeric at minimum concentration of 0.6g/L and at high concentration 2.0 g/L (Figure 3). Hence, it can be depicted that a moderate amount of turmeric between 0.8 – 1.0 g/L of turmeric can nullify the contamination in tissue culture studies (Figure 4). MS media with carbendazium has also showed the null contamination at 2.0 g/L. Further the carbendazium is a chemical compound, which can be hazardous, while the turmeric plays an important role as an effective antifungal/microbial agent.

Int J Dt '\' Biol I1 199 2]2(1997) 199 Mechanisms of normal and abnormal neurulation evidence from embryo culture studies ANGELEEN FLEMING, DIANNE GERRELLI, NICHOLAS D E GREENE and ANDREW J COPP' Neur[.]

valuable source of different kinds of useful products like wood, medicine, fiber, firewood and timber suitable for furniture, as well as decorative plants, pharmaceutical products and aestic items. In recent years the relevance of plant tissue culture methods has gained thrust to meet the growing demands for pharmaceutical industries since the genetic diversity of medicinal plants is decreasing hap hazardously. Conventional propagation and high demand of planting material are the major constraints for the large scale multiplication of medicinal species which can be met economically and efficiently in short span of time by in vitro propagation. Micropropagation is an alternative means of propagation that can be engaged in the conservation of flora in relatively shorter time. Tissue culture is useful for multiplying and conserving the useful species which are difficult to regenerate and thus can be saved from extinction. There is an only partial advancement explored at the research level to suggest tissue culture studies in Sterculiaceae. Conclusively, this appraisal is the first of its kind which emphasizes the procedures available for in vitro propagation along with some of the remarkable achievements carried in some pharmaceutically and commercially important plants by in vitro culture techniques of plants from this family.

MATERIAL AND METHODS: Eleven sugarcane clones viz. Thatta-10, AEC71-2011, AEC82-223, AEC82- 1026, NIA-98, BF-129, SPSG-26 and BL4 were used for tissue culture studies. Ten explants containing leaf premordia were taken from each genotype, sterilized by standard procedure (Siddiqui et al., 1988) and cultured on Modified MS medium (Murashige and Skoog, 1962) + 2mg/ 1 2,4-D, 3mg/1 2,4-D and 4mg/1 2,4-D with pH 5.8 and solidified with 0.8% Difco bacto agar for callusing. Comm- ercial sugar instead of Analar grade sucrose was used in the medium.

Cowpea is a dicotyledonous plant belonging to the family Fabaceae and sub-family, Fabiodeae. Cowpea is often called as "black-eyed pea" due to its black- or brown- ringed hylum. Vigna is a nutritious source of food, its grains contain about 25% Protein, especially rich in folate, potassium, iron, magnesium and the essential amino acids lysine and tryptophan. It is also used as animal fodder, cover crop and green manure. Cowpea is often called as "black-eyed pea" due to its black- or brown-ringed hylum. Vigna originated in West Africa India, Nigeria accounts for 70% of the world‟s production of cowpea beans. The plant tissue culture is also proving to be rich and novel sources of variability with a great potential in crop improvement without restoring to mutation or hybridization. The plant with long seed dormancy can be raised faster by in vitro clonal propagation. In the present study shooting was initiated from nodal and shoot tip explants on MS medium containing BAP alone used as phytoharmone source. Rooting in the form of organogenesis was obtained from leaf explants in the presence of NAA alone as phytoharmone.

The calcium content of soils is widely variable. In rich soil some 70-80% of the cations exchange complex may carry ions and in non-carbonate soils this may be a relatively large proportion of the total soil Ca. the equilibrium concentration of Ca in the soil solution is also high compared to other cations such as K. Calcium deficient sand culture study on Tephrosia purpurea shows, effects mostly on morphological features. Ca deficiency changes the activity of enzymes which helps in elongation of underground parts and retards growth of above ground portion.

found to improve significantly the growth of wheat, barley and oats in hydroponics (Shkolnik 1984). Since factors like nutrient concentrations, pH, ionic strength and other parameters are difficult to alter in soil culture, investigations through nutrient culture techniques have been practised. The present paper reports the prel im inary studies aimed at evaluating the influence of applied AI on plant toxicity and uptake of plant nutrients, namely, P.K, Ca, Mg, Cu, Fe, Mn and Zn by wheat seedlings using solution culture.

Forty of the mutants suffer from their defects early in kernel development and are blocked in embryogenesis before their primordia differentiate, or, if pri- mordia are formed,[r]

Nanoparticle in cancer treatment was studied using an in vitro model, where uptake of the polylactic-co-glycolic acid (PLGA) nanoparticles coated with polyvinyl alcohol (PVA) was shown to be 1.4 times less than that coated with vitamin E TPGS on a colorectal cancer cell line [45]. Recently our team demonstrated the effect of coating on iron oxide nanoparticle intercalated with Gallic acid in a cell culture-base study [46]. The magnetic nanoparticles with average size of 11.4 nm and coated with Polyethylene glycol (PEG) showed enhanced anti-cancer effect on breast cancer but not lung cancer compared to pure Gallic acid [46]. However, the same nanodelivery system containing Gallic acid coated with chitosan demonstrated lesser anti-cancer activity on both breast and colon cancer compare to the pristine Gallic acid [3]. Thus, cell culture studies allowed for the choice of better polymer on this nanoparticle containing Gallic acid in cancer treatment. It also helps in the screening of different cancer for which the delivery system will likely have better effect on.

Since a lot of variation is found in the betalain content of beet root obtained from seedling derived plants, this study was conducted to develop a protocol for in vitro multiplied red beet plants from seedlings shoot tips initially for standardization of protocol. Shoot tips of mature plants were in vitro multiplied to obtain clonal plants yielding uniform roots and high betalain content. From the field evaluation studies of both seedling and micropropagated plants, it was found that micropropagated plants were superior in growth, uniform roots and fairly high betalain production. From this study we could develop a protocol for uniform betalain yielding plants.

“Pixações, Graffiti, and the Writing of Inequality on São Paulo’s Walls.” Cross/words-Cross/roads: An Intersection of Language, Art and Culture Colloquium, San Diego State University, 2[r]

Lobola (bride price) is a cultural practice that has stood the test of time and it is still observed within the Shona culture (Wadesango et al., 2011; Padare & WAG, 2011; Kambarami, 2006; & Sibanda, 2011; Chireshe & Chireshe, 2010; Dura, 2015; Ansell, 2001; Armstrong, 1998; Chirawu, 2006). Lobola in the Shona culture is known as ‘pfuma’, ‘rovora’, ‘kutowora’ or ‘roora’ (Nkomo, 2014:41). For consistency, this thesis will use lobola from here on. Lobola is the transfer of property, usually in the form of livestock, by the husband or the guardian to the wife’s family as part of the marriage process (Nkomo, 2014:41). This is a custom that has had positive value in the Shona culture. Firstly, it has helped bring the two families of the woman and the man who are to be married together (Chireshe & Chireshe, 2010:212). Secondly, this cultural practice acts as a way of transferring the legal custody of the children born in the marriage to the father or his family, however, this was done to the exclusion of the mother’s family (p212). Thirdly the custom is said to be a sign of respect and gratitude to the parents of the bride (Dura, 2015:1). Despite the positive results that come from the practice research has found that lobola brings about the manifestation of gender inequality and the control of a woman’s sexuality within a marital context (Chireshe & Chireshe, 2010:212).

Muscle cultures from three unrelated patients with muscle phosphofructokinase (PFK; EC 2.7.1.11) deficiency (Glycogenosis type VII; Tarui disease) had normal PFK activity and normal morphology. Chromatographic and immunological studies showed that normal muscle cultures express all three PFK subunits, M (muscle-type), L (liver-type), and P (platelet-type) and contain multiple homotetrameric and heterotetrameric isozymes. Muscle cultures from patients lack catalytically active M subunit-containing isozymes, but this is compensated for by the presence of P- and L-containing isozymes. Despite the lack of muscle-type PFK activity, presence of immunoreactive M subunit was demonstrable by indirect immunofluorescence, suggesting a mutation of the structural gene coding for the M- subunit of PFK.

37, 1981 HAV-SPECIFIED PROTEINS AND RNA IN VERO CELLS The validity of the double-label and subtraction method to obtain net electrophoretic profiles of virus-specified proteins has been [r]

Despite migration, some enduring core beliefs and actions of South Asian culture have been identified, for instance, the relationship between men and women and attitudes to dating and marriage. Stuart and Ward (2011) argued that while South Asian migrants in New Zealand have the expertise for embracing the wider society by active engagement and participation, they also demonstrate firm intention to preserve their traditional culture and identity. Stuart and Ward (2009) stated in relation to South Asian immigrants’ attitude to the man-woman relationship, “Beliefs regarding dating practices can be one of the most significant points of contrast between the ethnic and host culture’s norms. In a number of non-Western cultures, marriage is the first point of intimacy with a member of the opposite sex. The practice of dating is not only foreign but also threatening, as it undermines religious and moral values” (p. 27). Kwak and Berry (2001) have noted, in their Canadian study, that immigrants from South Asia reveal the highest desire for cultural preservation after migration. They also found that their expectation is for traditionalism concerning marriage and family life. DeSouza (2010) reported similar findings for the New Zealand context and stated that for most South Asian migrants, marriage is an eternal bond and is recognised as more than a contract or an institution. She also pointed that in marriage women are expected to produce children as early as possible, and ideally to give birth of a male child who will continue the family blood and ensure keeping the family wealth in the family. Merali (2015) argued that arranged marriage is a significantly common custom in South Asian culture, in which parents, along with elderly relatives, makes the decision in finding suitable partners for the children. She acknowledged that, historically, the formal or informal exchange of money or dowry is present in the custom of arranged marriage, whereby the family of the male gains in the money exchange. Thus preference for male children and gender inequity have become part of South Asian cultural customs (Merail, 2015; DeSouza, 2010).

Surnamed „embryos’ saving method”, embryos culture is an in vitro technique used for over half of the century for saving the distant hybridization products, that would have degenerate in other conditions. Immature embryos culture is used for initiation of in vitro cultures imposed by the impossibility of using other explants for some of the plant species. Wheat is one of the crops that immature embryos culture technique is suitable for. This methods principle is based on aseptic embryos excision and their inoculation to an adequate culture medium. In vitro culture results depend in a greater manner of the basic culture medium and the hormonal balance used. Immature embryos isolated from two Romanian wheat cultivars – Dropia and Lovrin 41 – were inoculated for callus production on two types of basic media added with 2,4 D. The selected calluses were transferred on MS basic medium and several parameters were registered. Both cultivars emphasized a good callusing capacity, in a different percentage depending on the culture media used, such as 71,09 – 94,45%.. big differences between the cultivars regarding embriogenic callus frequency, shooting callus frequency and regenerated plants percentage were registered

© 2019 by the author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License (https:// creativecommons.org/licenses/ by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license. Citation: Rowse, T. 2019. Reconciliation as Public Culture: Taking Cultural Studies Beyond Ghassan Hage’s ‘White Nationalist’. Cultural Studies Review, 25:2, 104-108. http://dx.doi. org/10.5130/csr.v25i2.6884 ISSN 1837-8692 | Published by UTS ePRESS | https://epress. lib.uts.edu.au/journals/index. php/csrj

While a number of countries have taken into consideration the prevention, preparedness and management of natural disasters, there is still a need to provide safety measures upon the occurrence of disasters. In New Zealand, there is a disaster preparedness policy that came into effect after the impacts of the 2011 Christchurch earthquake, which resulted in 185 deaths and significant damage to property and human life (GNS Science 2011). Schoolchildren are amongst those that may be affected by death through natural disasters. In the United States of America (USA), the Council on School Health (2008) notes that studies focusing on recent natural disasters have concurred that there are several important deficiencies in school preparedness for emergencies. This prompted the US policymakers to come up with policy guidelines that redressed the identified deficits in the schools’ disaster preparedness. Developing a culture of safety management will ensure that schools are prepared for any kind of disasters that are likely to occur in their area.

Real and sustained reform of the health system is more likely to be achieved if organisations are able to draw on a repertoire of management styles and skills. To achieve this, primary care trusts need to place greater value on the skills of their facilitative managers. At the same time, policy makers and politicians need to consider the likely trade offs between achieving short term improvements in measurable performance and sustained change in the culture of NHS organisations. These aims are not mutually exclusive in policy terms—but they may feel that way to the managers and clinicians working in the front line of the service.

This research, with its application of the ‘3Ac’ design methodology, has demonstrated certain implications with regards to sustainable development. Firstly, it underscores the need to better understand the importance of the 17 Sustainable Development Goals -- not simply its content, but also its context, especially with regards to case studies from different countries. Secondly, it shows how design for sustainable development is an international trend. This trend is reinforced by millennials’ lifestyle trends, as most younger participants’ purchasing and spending patterns contribute to aspects of sustainable development. (The Positive Impact of Millennials’ Lifestyle Trends, 2018)