culture supernatants
Comparison of methods to detect Escherichia coli heat labile enterotoxin in stool and cell free culture supernatants
... In addition, the ELISA of stool supernatants detected more samples known to be positive by YAC assay of pooled culture supernatants than did the ELISA of culture supernatants, possibly o[r] ...
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Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system
... Bacterial cultures of TD4 and TF1 clones were induced and their culture supernatants and bacteria were col- lected. These samples were either directly precipitated with trichloroacetic acid (TCA), or ...
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Detection of Cell Wall Mannoprotein Mp1p in Culture Supernatants of Penicillium marneffei and in Sera of Penicilliosis Patients
... body is specific since it fails to react with any protein-form lysates of Candida albicans, Histoplasma capsulatum, or Cryptococcus neoformans by Western blotting. In addition, this Mp1p antigen-based ELISA is also ...
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Detection of Cell Wall Galactomannoprotein Afmp1p in Culture Supernatants of Aspergillus fumigatus and in Sera of Aspergillosis Patients
... cell culture supernatant at a level that can be detected by Western ...cell culture supernatant of ...cell culture supernatants of the other eight fungi gave negative ...
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A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations
... ampicillin. A single colony was used to inoculate 20 mL LB media and grown over night at 37 °C. To inoculate the main culture (1 L) in auto induction medium, 1 mL of this pre-culture was used [27]. Cells ...
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An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells
... ADSC: Adipose tissue – derived mesenchymal stem cell; BCA: Bicinchoninic acid assay; BMSC: Bone marrow – derived mesenchymal stem cell; BSA: Bovine serum albumin; hMSC: Human mesenchymal[r] ...
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Identification of Acinetobacter species
... Siderophore utilization bioassays showed the presence of 2,3-dihydroxybenzoic acid in the growth medium, and neither enterobactin nor aerobactin was detected in culture supernatants obta[r] ...
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Characterization of Recombinant Flaviviridae Viruses Possessing a Small Reporter Tag
... of culture supernatants obtained from the transfected ...The supernatants were collected at 24, 48, and 72 h ...of culture supernatants was used to infect naive ...the culture ...
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Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus
... Cell culture supernatants harvested from group C rotavirusinfected MA104 cells passages 13 and 17, as well as the gut virulent Cowden group C rotavirus, and cell culture-propagated OSU g[r] ...
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Chen, Xiao (2011): Development and characterisation of an immunochemical test system for the determination of bacterial signal molecules (N-acylated homoserine lactones). Dissertation, LMU München: Fakultät für Biologie
... Detection of AHL quorum sensing molecules in Burkholderia cepacia culture supernatants with enzyme-linked immunosorbent assays, Analytical Bioanalytical Chemistry, vol[r] ...
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In vitro infection of natural killer cells with different human immunodeficiency virus type 1 isolates.
... Viral replication was demonstrated by detection of p24 antigen intracellularly and in culture supernatants, by the presence of HIV DNA within infected cells, and by the ability of supern[r] ...
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Vesicular Polysaccharide Export in Cryptococcus neoformans Is a Eukaryotic Solution to the Problem of Fungal Trans-Cell Wall Transport
... from culture supernatants (white bar). Supernatants obtained from these suspensions were assayed as a control of vesicle integrity, and in fact, GXM was detected at low levels in these preparations ...
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Serotyping of Cryptococcus neoformans by dot enzyme assay
... using culture filtrates was evaluated in a study of a group of chemically and serologically defined isolates of ...1). Culture supernatants were reactive to factor serum 1 ...
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The First Transmembrane Domain of the Hepatitis B Virus Large Envelope Protein Is Crucial for Infectivity
... of culture supernatants that were collected between days 6 and 12 post- transfection were precipitated with 8% PEG, disrupted in sample buffer (Invitrogen), and analyzed as described ...of culture ...
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Cytomegalovirus CC Chemokine Promotes Immune Cell Migration
... clarified supernatants produced FIG 1 (A, C, and D) RCMV r129 is expressed with late viral expression ...in culture supernatants and cellular lysates from RCMV-infected cells by immunoblotting using ...
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Induction of monocyte expression of tumor necrosis factor alpha by the 30 kD alpha antigen of Mycobacterium tuberculosis and synergism with fibronectin
... in supernatants of cultured monocytes. Immunoreactive TNF- a in culture supernatants was determined by ...C. Culture supernatants and recombinant human TNF- a (Gen- zyme ...
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DIFFERENTIAL PRODUCTION OF IgM A ND IgA RHEU MATOID FACTORS IN PERIPHERAL BLOOD AND SYNOVIUM OF PATIENTS WITH RHEUMATOID A RTHRITIS
... Quantitation of in vitro induced IgM-RF and IgA-RF Lymphocytes isolated from peripheral blood and synovial fluid of three seropositive patients were stimulated in vitro with PMA and culture supernatants ...
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High resolution metabolomics to discover potential parasite specific biomarkers in a Plasmodium falciparum erythrocytic stage culture system
... vitro culture samples. Methods: Supernatants were collected at 12 hours interval from 3% haematocrit in vitro 48-hour time-course asynchronized culture system of ...
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Expression of endoglucanases in Pichia pastoris under control of the GAP promoter
... the culture was approx. 4 ml). Then a sample of 100 μl culture was taken out, 100 μl 40% (v/v) methanol was added and the cultures were incubated for another 24 ...Cell-free culture ...
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Brucella abortus induces TNF-α-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases
... in culture supernatants from astrocytes stimulated with HKBA when compared with the unstimulated cells, as assessed by zymography and gelatinolytic activity test (Figure 2A and B, respect- ...ture ...
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