Cytochrome oxidase subunit 2
Cytochrome oxidase subunit 2 gene allows simultaneous detection and typing of Trypanosoma rangeli and Trypanosoma cruzi
... of Platinum Taq DNA polymerase (Invitrogen) in the buffer provided by the manufacturer. Amplification was processed in a Techne TC-512 thermocycler with initial denaturation at 94°C for 1 min, followed by 30 cycles (94°C ...
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Identification and Characterization of the Novel Subunit CcoM in the cbb3 Cytochrome c Oxidase from Pseudomonas stutzeri ZoBell
... induced with 0.2% (wt/vol) L -arabinose. After 4 h of induction, the P. stutzeri ZoBell cells were electrotransformed with approximately 5 g of linearized DNA and incubated in super-optimal-broth (SOC) medium without ...
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Reconstructing mammalian phylogenies : a detailed comparison of the cytochrome b and cytochrome oxidase subunit I mitochondrial genes
... At the level of Order cyt b grouped correctly 94.31% of 211 mammal species and COI gave a slightly lower percentage of 93.36%. For COI six placental mammal Orders were split with maximum correct groupings ranging from ...
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A study of cytochrome C oxidase subunit 1 polymorphism in Malay population
... c oxidase subunit 1 DNA size is around 1548 bp which has ...addition, Cytochrome c oxidase protein (EC ...reduced cytochrome c to the oxygen (Valnotet ...of cytochrome c ...
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Genomic and Population Analyses of the Mating Type Loci in Coccidioides Species Reveal Evidence for Sexual Reproduction and Gene Acquisition
... a cytochrome c oxidase subunit VIa gene (COX13) and a DNA lyase gene (APN2) that have limited similarity to their counterparts in the other ...
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Characterization of mitochondrial DNAs from the dekkera/brettanomyces and eeniella genera
... ATPase subunit 6 and cytochrome oxidase subunit I genes and a second smaller ...SrRNA/ cytochrome b gene cluster and the ATPase subunit 9 (see ...the cytochrome ...
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DNA barcoding: complementing morphological identification of mosquito species in Singapore
... spacer 2 [14], cytochrome b oxidase [15,16], 12S rRNA [17,18] and nicotinamide adenine dinucleotide dehydrogenase [19,20] as target ...mitochondrial cytochrome c oxidase subunit ...
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Molecular identification of potential leishmaniasis vector species within the Phlebotomus (Euphlebotomus) argentipes species complex in Sri Lanka
... the Cytochrome oxidase subunit I sequence data with 4 classes of variable sites and the HKY + G model of nucleotide substitution (Phyml ...Figure 2), This clade contained only those flies ...
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Karyotypic assignment of Sri Lankan Anopheles culicifacies species B and E does not correlate with cytochrome oxidase subunit I and microsatellite genotypes
... Thirty nine unrelated individuals from the 3 populations were characterised by the Y-chromosome karyotype and used in the study. Five microsatellite markers capable of amplification from An. culicifacies sp. A and sp. B ...
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Surveillance of Helminthes and Molecular Phylogeny of Fasciola Gigantica Infecting Goats in Sadat District, Egypt
... DNA, cytochrome oxidase subunit 1 (CO1) and Internal Transcribed Spacer 2 (ITS2) of the ribosomal RNA gene were used to identify Fasciola species that infects cattle in Qena province, Upper ...
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Analysis of the cytochrome c oxidase subunit II (COX2) gene in giant panda, Ailuropoda melanoleuca.
... The phylogenetic analysis performed using DNA substitution model showed that the 17 giant panda sequences obtained in the present study were clustered within the Ursidae family (Figure 2), suggesting that the COX2 ...
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The cyclope Gene of Drosophila Encodes a Cytochrome c Oxidase Subunit VIc Homolog
... ACTC-3⬘ (640/621). PCR products were cloned into pGEM Genome Annotation Database of Drosophila identified plasmid (Promega, Madison, WI) and sequenced using primer nine putative nuclear COX subunits. 2, SP6 or T7 ...
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Genetic Diversity in the Camel Tick Hyalomma dromedarii (Acari: Ixodidae) Based on Mitochondrial Cytochrome c Oxidase Subunit I (COI) and Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD PCR)
... In the RAPD-PCR, the banding pattern produced by each primer on the agarose gel was scored as one or zero (one for the presence of a band and zero for the absence of the band). Band scores were analyzed using cluster ...
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Myocardial insufficiency is related to reduced subunit 4 content of cytochrome c oxidase
... catheterization. After exclusion of coronary artery dis- ease, arterial hypertension and cardiac valve disease, up to 9 left ventricular (LV) endomyocardial specimens were obtained with a flexible Cordis bioptome from ...
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MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF CULEX QUINQUEFASCIATUS BY CYTOCHROME C OXIDASE SUBUNIT II
... DNA Amplification by (PCR) was used to amplify a segment of the COII gene using the primer pair table (1) to find the optimal condition has identified for (Initial denaturation and annealing) after a work several ...
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Peroxisome proliferator-activated receptors γ/mitochondrial uncoupling protein 2 signaling protects against seizure-induced neuronal cell death in the hippocampus following experimental status epilepticus
... protein, cytochrome c oxidase subunit IV (COX ...Bcl-2, cytochrome c and PPARγ (Santa Cruz Biotechnology) or β-actin (Chemicon, Temecula, CA, ...Bcl-2, cytochrome c, PPARγ ...
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Mode and tempo of molecular evolution in the nematode caenorhabditis: cytochrome oxidase II and calmodulin sequences.
... Through direct sequencing methods, the mitochondrial gene for cytochrome oxidase subunit two (CO 11) and the single-copy nuclear gene for calmodulin were compared among s[r] ...
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Time course of liver mitochondrial function and intrinsic changes in oxidative phosphorylation in a rat model of sepsis
... the cytochrome c oxidase stoichi- ometry and/or activity [17, 18], the proton leak activity or the transcription of the UCP2 gene, and an increase in mitochondrial non-coupled respiration with ...
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Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next generation sequencing
... The phylogeny inferred from partial cox1 sequences corresponds to the tree topography described by Dusit- sittipon and colleagues [36]. Numerous haplotypes from different clades detected in China, Taiwan, Thailand, ...
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Molecular identification of cetaceans from the West Atlantic using the E3-I5 region of COI.
... Partial COI sequences (495 bp) from the E3-I5 region (locus 685 to 1179) were obtained for seven cetacean species (Figure 1). The specimens were sampled between 1986 and 1996 along the coasts of Newfoundland ...
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