Cytosolic and calcium independent phospholipase A2
Oligomeric amyloid-beta induces MAPK-mediated activation of brain cytosolic and calcium-independent phospholipase A2 in a spatial-specific manner
... Alzheimer ’ s disease (AD) is histopathologically characterized by the build-up of fibrillar amyloid beta (A β ) in the form of amyloid plaques and the development of intraneuronal neurofibrillary tangles consisting of ...
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Isolation of a human myocardial cytosolic phospholipase A2 isoform Fast atom bombardment mass spectroscopic and reverse phase high pressure liquid chromatography identification of choline and ethanolamine glycerophospholipid substrates
... minor cytosolic calcium-independent phospholipase A2 isoform that hydrolyzes plasmenylcholine and phosphatidylcholine substrates at similar rates and possesses a pH optimum of ...major ...
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Role of cytosolic calcium independent plasmalogen selective phospholipase A2 in hypoxic injury to rabbit proximal tubules
... of calcium-independent phospholipase A2 (PLA2) enzymes has been described in the heart, the pathogenetic role of this enzyme(s) in hypoxic cell injury has not been previously examined in any ...
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Inhibition of cytosolic phospholipase A2 prevents prion peptide-induced neuronal damage and co-localisation with Beta III Tubulin
... Results from at least three independent experiments are expressed as mean ± standard deviation (S.D.) unless sta- ted otherwise. Colocalisation of molecules in confocal microscopy was calculated using Pearson’s ...
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Inhibition of cyclin-dependent kinase 5 affects early neuroinflammatory signalling in murine model of amyloid beta toxicity
... of cytosolic phospho- lipase A2 (cPLA2) and lysophosphatidylcholine (LPC) release, by which it activated the surrounding microglia and astrocytes ...
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Predicting the mechanism of phospholipidosis
... of phospholipase activity and enhanced cholesterol biosynthesis are likely to be important ...phosphodiesterase, phospholipase A2 and lysosomal phospholipase A1 are shown to be likely targets ...
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Phospholipase A/Acyltransferase enzyme activity of H-rev107 inhibits the H-RAS signaling pathway
... H-rev107 activity that suppressed H-RAS acylation is eliminated by AACOCF3 and MAFP, but not by BEL. The inhibitory effect of AACOCF3 and MAFP on H- rev107 is similar to a previous study [3]. The results indicate that ...
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The activity of cytosolic phospholipase A2 is required for the lysis of adenovirus-infected cells by tumor necrosis factor.
... This conclusion is based largely on the activity of an antisense oligonucleotide corresponding to cPLA2, which completely inhibited the release of both [3H]arachidonic acid and 51Cr, ind[r] ...
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Cytosolic group IVA phospholipase A2 inhibitors, AVX001 and AVX002, ameliorate collagen induced arthritis
... In response to the cPLA2α inhibitors AVX001 and AVX002, the arthritis index that describes the number of inflamed joints and the degree of joint swelling and erythema, was significantly [r] ...
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Suppressive effect of secretory phospholipase A2inhibitory peptide on interleukin 1β induced matrix metalloproteinase production in rheumatoid synovial fibroblasts, and its antiarthritic activity in hTNFtg mice
... : cytosolic phospholipase A 2 ; cpm: counts per minute; DMARD: disease-modifying anti-rheumatic drug; DMEM: Dulbecco's modified eagle medium; DMSO: dimethyl sulfoxide; ELISA: enzyme- linked immunosorbent ...
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Interleukin 1 alpha causes rapid activation of cytosolic phospholipase A2 by phosphorylation in rat mesangial cells
... II phospholipase A2 ...of calcium inophore, [3H]arachidonate release is increased significantly as early as 5 min after addition of IL-1, and IL-1 concurrently stimulates a Ca(2+)-dependent ...
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The VP1 Unique Region of Parvovirus B19 and Its Constituent Phospholipase A2-Like Activity
... their phospholipase A2 activity ...pholipase A2 activity. The reason that parvovirus B19 has phospholipase A2 activity as part of the virion remains unex- ...the phospholipase ...
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Inherited human group IVA cytosolic phospholipase A2 deficiency abolishes platelet, endothelial, and leucocyte eicosanoid generation
... mediators primarily produced by COX and LOX path- ways. 12-HETE levels were somewhat lower in TRAP- 6–stimulated blood as compared to collagen-stimulated blood, consistent with reports that 12-LOX activation is linked to ...
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Cytosolic phospholipase A2 plays a crucial role in ROS/NO signaling during microglial activation through the lipoxygenase pathway
... anti-ionized calcium-binding adapter molecule 1 (Iba-1) antibodies (019–19741) was purchased from Wako BioProducts (Richmond, VA), Alexa Fluor 488® phalloidin from Life Technologies (Carlsbad, CA), and ...
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Effect of Kaempferol on Cyclooxygenase 2 (Cox2) and Cytosolic Phospholipase A2 (cPLA2) Protein Expression in BALB/c Mice
... Kaempferol, a phytochemical belonging to flavonoids of, is known to be able to alleviate diseases such as cancer. 12 Kaempferol also has many beneficial roles, including inhibiting matrix metalloproteinases and ...
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Increase of cytosolic phospholipase A2 as hydrolytic enzyme of phospholipids and autism cognitive, social and sensory dysfunction severity
... SPSS computer program was used. Results were expressed as mean ± SD and all statistical comparisons were made by means of independent t-test with P ≤0.05 considered as significant. To test the specificity and sen- ...
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Subcellular characteristics of phospholipase A2 activity in the rat kidney Enhanced cytosolic, mitochondrial, and microsomal phospholipase A2 enzymatic activity after renal ischemia and reperfusion
... Ischemia alone did not change the gel filtration chromatography patterns of PLA2 activity, but ischemia and reperfusion resulted in the appearance of a new peak of activity in cytosolic and mitochondrial fractions ...
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Cytosolic phospholipase A2 regulates Golgi structure and modulates intracellular trafficking of membrane proteins
... (35). Phospholipase D is present in Golgi membranes (36), is activated by ADP-ribosyla- tion factor (37), and is responsible for catalyzing the transphosphatidylation reaction of phosphatidic acid and ...
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Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer
... Previous work has indicated hydrogen peroxide is a potent activator of cPLA2 activity, partly due to phosphorylation through the MAPK pathway (van Rossum et al. 2004). Incubation of 4175-Luc+ cells with either ionomycin ...
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Cytosolic phospholipase A2 in hypoxic pulmonary vasoconstriction
... Cytosolic phospholipase A 2 (cPLA 2 ) releases arachidonic acid (AA) from phospholipids in cell membranes. To assess the role of cPLA 2 in hypoxic pulmonary vasoconstriction (HPV), we measured the increase ...
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