cytosolic fraction
MOLECULAR WEIGHT DETERMINATION AND METAL ION REQUIREMENT OF PHOSPHATIDATE PHOSPHOHYDROLASE PURIFIED FROM CYTOSOLIC FRACTION OF RAT LIVER
... from cytosolic fraction of rat liver was purified to homogeneity having specific activity of ...of cytosolic PAP is a hexamer of identical subunits and that charge density plays an important role in ...
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Nitric oxide synthase activity in human trophoblast, term placenta and pregnant myometrium
... NOS has been identified in human placenta at term as well as primordial placenta [14,15]. The NOS activity in these tissues was found to be highly Ca-dependent sug- gesting constitutive NOS. The activity was largely ...
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Electroacupuncture at different frequencies (5Hz and 25Hz) ameliorates cerebral ischemia reperfusion injury in rats: possible involvement of p38 MAPK mediated anti apoptotic signaling pathways
... the cytosolic fraction in the cortical penumbra; however, this expression was effectively restored by EA-5Hz and EA-25Hz 7 days after ...to cytosolic p-p38 MAPK ...
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Role of Cell-Type-Specific Endoplasmic Reticulum-Associated Degradation in Polyomavirus Trafficking
... through the polyacrylamide gel matrix. We harvested infected cells under alkylating conditions, to prevent any postharvest disulfide bond isomerization, at various time points after in- fection and isolated the ...
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Delayed Cytosolic Exposure of Japanese Encephalitis Virus Double-Stranded RNA Impedes Interferon Activation and Enhances Viral Dissemination in Porcine Cells
... the cytosolic viral RNA content of LLC-MK2 and PS cells through subcellular frac- ...the cytosolic fraction of LLC-MK2 cells had already peaked by 18 h ...the cytosolic viral RNA titer in PS ...
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Effects of hypoxia–cadmium interactions on rainbow trout (Oncorhynchus mykiss) mitochondrial bioenergetics: attenuation of hypoxia induced proton leak by low doses of cadmium
... 13,000 g pellet mitochondrial fraction 13,000 g supernatant cytosolic fraction adenine nucleotide translocase cadmium citrate synthase 2,6-dichlorophenol indophenol 2,4-dinitrophenol 5,5[r] ...
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Solubility of vesicular stomatitis virus M protein in the cytosol of infected cells or isolated from virions.
... M protein 50 jig/ml purified from [35S]methionine-labeled virions in buffer containing 0.65 M NaCI was diluted with either cell lysis buffer A, cytosolic fraction from uninfected cells a[r] ...
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Cytosol- and clathrin-dependent stimulation of endocytosis in vitro by purified adaptors.
... Perforated A431 cells were incubated in the presence or absence of crude cytosol 0.9 mg/ml, a cytosolic fraction enriched in clathrin 25 #g/mi, and/or purified APs 250 #g/ml for 30 min a[r] ...
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Growth factor dependent initiation of DNA replication in nuclei isolated from an interleukin 3 dependent murine myeloid cell line
... deprivation. Cytosolic and membrane-containing subcellular fractions were prepared from proliferating NFS/N1-H7 ...a cytosolic fraction, and a membrane-containing fraction ...
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Phosphorylation of apoptosis repressor with caspase recruitment domain by protein kinase CK2 contributes to chemotherapy resistance by inhibiting doxorubicin induced apoptosis
... the cytosolic fraction in human prostate carcinoma cells under androgens and/or growth factors stimuli [34]; CK2α targets to nuclear matrix in response to heat shock may serve a protective role in the cell ...
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Subcellular characteristics of phospholipase A2 activity in the rat kidney Enhanced cytosolic, mitochondrial, and microsomal phospholipase A2 enzymatic activity after renal ischemia and reperfusion
... in cytosolic, mitochondrial, and microsomal fractions of rat kidneys were characterized under control conditions, after ischemia, and subsequent to ischemia and ...the cytosolic fraction: a high ...
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Regulation of lipoprotein lipase translation by epinephrine in 3T3 L1 cells Importance of the 3' untranslated region
... The control cytosolic fraction also inhibited translation, when compared to the addition of no cell extract, and the examination of the control and epinephrinetreated cell extracts relat[r] ...
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Identification of the zinc finger 216 (ZNF216) in human carcinoma cells: a potential regulator of EGFR activity
... the cytosolic fraction and an accumulation of phosphorylated EGFR (pEGFR) more in the nuclear than in the cytosolic fraction compared to NIH3T3-EGFR ...the cytosolic compartment and its ...
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Proteinase 3–dependent caspase 3 cleavage modulates neutrophil death and inflammation
... the cytosolic fractions (50 mg) of either cultured HeLa cells or freshly isolated neutrophils (Supple- mental Figure 1; supplemental material available online with this article; ...purified cytosolic ...
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High resolution preparation of monocyte-derived macrophages (MDM) protein fractions for clinical proteomics
... for fraction- ating proteins on the basis of their hydropathicity ...the fraction analysed and the protein spot identified in the ...obtained fraction by SELDI-TOF-MS for hepcidin content, and we ...
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Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss)
... (the cytosolic form) is modulated by altering the abundance of the protein, ...1997). Cytosolic PEPCK gene transcription is positively regulated by glucagon (through cyclic AMP) and glucocorticoids, whereas ...
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PubMedCentral-PMC4786427.pdf
... expanding the replicative niche and possibly dissemination. So why would the host undergo a pro- cess that is so potentially deleterious? In cancer biology, trogocytosis of pMHC-I and pMHC-II results in a cytotoxic T ...
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Horizontal Transmission of Cytosolic Sup35 Prions by Extracellular Vesicles
... OptiPrep density gradient. For the discontinuous iodixanol gradient, a slightly modified form of a previously published protocol was used (71, 72). Briefly, 8.3 ml of the 60% (wt/vol) stock OptiPrep solution (Sigma) was ...
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Requirements for the Selective Degradation of Endoplasmic Reticulum-Resident Major Histocompatibility Complex Class I Proteins by the Viral Immune Evasion Molecule mK3
... The mK3 molecule of gammaherpesvirus 68, although re- lated to other K3 family members in terms of domain organi- zation, differs with respect to its site of action. Expression of mK3 results in decreased cell surface ...
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Effects of bafilomycin A1 on cytosolic pH of sheep alveolar and peritoneal macrophages: evaluation of the pH regulatory role of plasma membrane V ATPases
... membrane V-ATPases in m f of other animal species is uncertain. Further, no study to date has examined the plasma membrane V-ATPase activities of more than one mf type from any single animal species. These are meaningful ...
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