the cytotoxic concentration of the compound that reduces cell viability by 50%
Cytotoxic assays for Lung and Colon cancer
... of 50 ml. The cell pellet, obtained by centrifugation was suspended in 10 ml of medium to make a single cell suspension, viable cells density being counted by trypan blue exclusion in a ...
17
β-escin selectively targets the glioblastoma-initiating cell population and reduces cell viability
... natural compound β-escin as a selective inhibitor of GIC ...significantly cytotoxic in nine patient-derived GIC, whilst exhibiting no substantial effect on the other human cancer or control cell ...
15
Concentration effects of grape seed extracts in anti oral cancer cells involving differential apoptosis, oxidative stress, and DNA damage
... cancer cell lines. For example, high concentrations (50–600 μg/ml) of GSE of Vitis vinifera were found to inhibit cell prolifera- tion and induce apoptosis of the KB cells but less harm- ful to ...
9
4 Cyano 1,3 oxazole 5 sulfonamides as Novel Promising Anticancer Lead Compounds
... the concentration required to inhibit 50% of cells, the TGI value (cytostatic activity) is the concentration of the compound resulting in total growth inhibition, and LC50 value ...
16
The little R cell that could
... each cell fate decision within an ommatidium (Tomlinson and Ready, ...pre-R7 cell initiates nuclear migration and moves to the apical surface but fails to move basally at the proper time and instead assumes ...
6
Original Article Resveratrol relive osteoarthritis through reducing inflammatory reactions
... The viability of chondrocytes was detected by the MTT assay, and the expression levels of TNF-α, IL-6, p-PI3K, and p-AKT were measured by ...the viability of cells ...the viability of chondrocytes ...
7
Overexpression of Mst1 reduces gastric cancer cell viability by repressing the AMPK-Sirt3 pathway and activating mitochondrial fission
... (1:1,000, Cell Signaling Technology, ...(1:1,000, Cell Signaling Tech- nology, ...(1:1,000, Cell Signaling Technology, ...(1:1,000, Cell Signaling Technology, ...(1:1,000, Cell ...
15
The PI3K inhibitor GDC-0941 displays promising in vitro and in vivo efficacy for targeted medulloblastoma therapy
... For validation of GDC-capacity to inhibit the PI3K/ AKT signaling pathway, the phosphorylation status of the most prominent PI3K downstream signaling molecule, AKT, was selected as a valid marker for PI3K activity [14]. ...
12
<p>The value of the plasma circulating cell-free DNA concentration and integrity index as a clinical tool for prostate cancer diagnosis: a prospective case–control cohort study in an Iranian population</p>
... short-fragment concentrations and their integrity is the detection of low concentration DNA pieces in plasma by an easy assay with direct q-RT-PCR. Regardless of the differences in the stages of the disease and ...
8
Potent antiplasmodial extracts and fractions from Terminalia mantaly and Terminalia superba
... anolic extract from the leaf of Terminalia superba against the chloroquine-sensitive P. falciparum F32 strain. This value is about five to ninefold greater than that of similar extract tested in this study against PfINDO ...
9
Cytotoxic, antibacterial and antioxidant activity of triterpenoids from Kopsia singapurensis Ridl
... Each compound was prepared at 1 mg/mL in ...mg/mL. Concentration in the first row was 500 µg/mL of sample in each well and was serially diluted (two fold dilution) in 96 micro-well plates to varying ...
8
Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery
... Moreover, DPTE is commercially available and pro- vides a stable membrane anchoring of peptides to the lipid bilayer in comparison with single fatty acid chain derivatives. The resulting lipopeptides are soluble in ...
14
Biological Activity of Levan Produced from Rhizospheric Soil Bacterium Brachybacterium phenoliresistens KX139300 Mohammad M.F. Al-Halbosiy| Zaid A. Thabit| Safaa Al-Deen A. S.Al-Qaysi| Tarek A.A. Moussa
... Bacterial inoculum was prepared by the preparation of 50 ml of sterilized nutrient broth that contained in 250 ml in volume Erlenmeyer conical flask. This medium was inoculated by one loopful of bacterial ...
6
Antioxidant and cytotoxic activities of three species of tropical seaweeds
... This study aimed to evaluate the cytotoxic and antioxi- dant activities of three seaweeds, Padina tetrastromatica, Caulerpa racemosa and Turbinaria ornata. P. tetrastro- matica is a brown alga which grows in ...
14
Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib
... Enzalutamide is an AR antagonist and has been approved by FDA for the treatment of patients with metastatic prostate cancer [29, 30]. Next, we investigate whether treatment of CDK4/6 inhibitor in combination with AR ...
11
A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection
... Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bo- vine Serum (FBS), Antibiotic Antimycotic Solution (AAS), Polyethyleneimine (PEI, 25 kDa), Dimethyl Sulfoxide (DMSO) were purchased from Sigma-Aldrich (MO, USA). The ...
8
Label-Free, Flow-Imaging Methods for Determination of Cell Concentration and Viability
... In order to reduce the probability of detection of optically overlapping particles, cell-containing samples were first dilut- ed 4-fold with particle free NaCl 0.9% m/v + HSA-20% 2% v/v. The diluted samples were ...
10
<p>IP10-CDR3 Reduces The Viability And Induces The Apoptosis Of Ovarian Cancer Cells By Down-Regulating The Expression Of Bcl-2 And Caspase 3</p>
... Cells were lysed in RIPA buffer in an ice bath for 30 mins. The lysate was centrifuged at 4°C and 10,000 rpm for 10 mins and the supernatant was collected carefully to obtain total protein. Protein concentration ...
10
The novel dual PI3K/mTOR inhibitor NVP-BGT226 displays cytotoxic activity in both normoxic and hypoxic hepatocarcinoma cells
... The frequent activation of the PI3K pathway in cancer and its crucial role in cell growth and survival has made it a much relevant desired target for pharmacologic intervention. The first PI3K pathway-targeted ...
14
CYTOTOXIC AND ANTHELMINTIC ACTIVITIES OF METHANOLIC EXTRACT OF PARTHENIUM HYSTEROPHORUS L.
... 3-(4,5–dimethylthiazol–2–yl)–5–diphenyl tetra- zolium bromide (MTT), Fetal Bovine Serum (FBS), Phosphate Buffered Saline (PBS), Dulbecco’s Modified Eagle’s Medium (DMEM) and Trypsin were obtained from Sigma Aldrich Co, ...
5