D2-40
Decreased D2-40 and increased p16INK4A immunoreactivities correlate with higher grade of cervical intraepithelial neoplasia
... The D2-40 immunor- eactivity observed in the lymphatic endothelium of the cervical stroma in this study acts as an internal control, indicating that the loss of D2-40 protein expression in the ...
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Original Article Immunohistochemical evaluation of CD10, podoplanin (D2-40), and Ki-67 expression in basaloid epidermal proliferation of nevus sebaceous with comparative analysis of basal cell carcinoma and trichoepithelioma
... staining localized to the basal cell layer of the tumor nests, while BCC showed low reactivity (22.2%) with focally positive staining at the periphery of the tumor cell aggregates. How- ever, our results differ in term ...
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Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver- Re-evaluations of microlymphatic abnormalities
... Blood vessels are identifiable using several immuno- histochemical methods. Immunostaining for a smooth muscle actin, factor VIII-associated antigen, and alkaline phosphatase (ALPase) are strongly positive in blood ves- ...
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Lymphangiogenesis as a Prognostic Marker in Breast Cancer Using D2 40 as Lymphatic Endothelial Marker—A Preliminary Study
... using D2-40 in breast cancer patients for prognostic ...using D2-40 can be used as a single prognostic marker in primary breast cancer but needs multicentric study and a longer follow up for ...
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Original Article Possibility of D2-40 as a diagnostic and tumor differentiation-suggestive marker for some of phosphaturic mesenchymal tumors
... for D2-40 are summarized in Table ...with D2-40, with a proportion score ranging from 1+ (three cases, 21%), 2+ (five cases, 36%), to 3+ (four cases, 29%) and an intensity sore ranging from 1+ ...
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Lymphatic vessels around the uterus: D2-40 (podoplandin) immunohistochemistry using elderly cadavers
... viscera. Five to seven macroscopic slices (15 mm thick) were prepared from each of the hemiblocks after bisection along the midsagittal line. After per- forming routine procedures for paraffin-embedded histology, 5 to 7 ...
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Original Article D2-40: an additional marker for myoepithelial cells of breast and the precaution in interpreting tumor lymphovascular invasion
... Abstract: D2-40 is a recently available mouse monoclonal antibody specific for human podoplanin and has been used in identifying lymphovascular invasion (LVI) of ...for D2-40, calponin and p63 ...
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Lymphangiosis carcinomatosa in squamous cell carcinomas of larynx and hypopharynx – value of conventional evaluation and additional immunohistochemical staining of D2 40
... The decision between lymphatic and blood vessel inva- sion shows a high interobserver variability, because the morphological discrimination between small lymphatic and blood vessels is difficult. The question was, ...
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Vascular endothelial growth factor (VEGF)-C, VEGF-D, VEGFR-3 and D2-40 expressions in primary breast cancer: Association with lymph node metastasis
... the D2-40 evaluation, the number of D2-40 stained lymphatic vessels were counted on 10 intra-tumoral (located within the tumor) and 10 peri-tumoral (located in the adjacent non-tumoral pe- ...
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Use of Immunomarkers D2-40 and CD31 in Detection of Lymphovascular Invasion in Breast Carcinoma
... D2-40 is a sensitive and specific marker to detect tumour cells in lymphatic vessels (1)(2) . It selectively and specifically stains lymphatic endothelial cells. CD31 is a 130 kDa transmembrane glycoprotein ...
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Original Article Podoplanin (D2-40): A New Immunohistochemical Marker for Reactive Follicular Dendritic Cells and Follicular Dendritic Cell Sarcomas
... Both D2- 40 and CD21 highlighted FDC network of germinal centers in the majority of RLH cases (22/25 for D2-40, 23/25 for ...CD21). D2-40 stained the FDCs in all cases of PTGCs ...
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Original Article D2-40 expression in ALK-positive anaplastic large cell lymphoma resembles CD30 expression: a potential diagnostic pitfall
... [1]. D2-40 is a monoclonal antibody against an oncofetal antigen, the M2A antigen, a 40-kDa transmembrane sialoglyco- protein with an O-linked, but not N-linked, sim- ple mucin-type carbohydrate ...
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Tube-Like Structures with Co-Expression of D2-40 and CD34: Newly Formed Vasculatures?
... Experimental findings: As cytokeratin (CK)-19 is a well-recognized stem cell marker and CK-19-positive cells are frequently detected in the peripheral blood of patients with meta- static cancer, our recent studies have ...
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Original Article Lymphangigenesis in human epithelial ovarian cancer is related to the formation of ascites
... to D2-40 (Maxin, Fuzhou, China) and Ki-67 (Maxin, Fuzhou, China) se- quentially with the double immunohistochemi- cal kit (Maxin, Fuzhou, ...human D2-40 (1:400) was applied at 4 Centi-degree ...
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Observational study of coagulation activation in early breast cancer: development of a prognostic model based on data from the real world setting
... On the other side of the medal, biomarkers related to coagulation disorders have shown prognostic relevance in lung, colorectal, ovarian cancer and glioblastoma, independently on the occurrence of VTE [8–11]. When ...
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www.ijcep.com /IJCEP1108006 Case Report Immunohistochemical profile of normal mesothelium and histiocytic/methothelial hyperplasia: a case report
... (calretinin, D2-40, CK5/6), CD68, and T-cell ...(calretinin, D2-40, CK5/6) in HMH indicate that these antigens are not useful in the distinc- tion between reactive mesothelial proliferation ...
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Emerging clinical applications of selected biomarkers in melanoma
... specificity. D2-40 exposes vascular invasion in a subset of melanomas, and use of double IHC marker MiTF/D2-40 or Sox10/D2-40 confirms the presence or absence of melanoma cells ...
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Cavernous Hemangioma-Like Kaposi Sarcoma: Histomorphologic Features and Differential Diagnosis
... Aim. Cavernous hemangioma-like Kaposi sarcoma is a rare morphologic type of Kaposi sarcoma. So far there are no cases in the literature defining the histological features of this morphologic spectrum in detail. In this ...
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Original Article CyclinD1 promotes lymph node metastasis by inducing lymphangiogenesis in human ovarian carcinoma
... visionTM Detection system (Envision kit; Gene technology, Shanghai, China) according to the manufacturer’s protocol. Briefly, endogenous peroxidase activity was blocked with 3% hydro- gen peroxide and the sections were ...
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Assessment Of Lymphatic Vessel Density In Breast Carcinoma Using Immunohistochemical Marker D2-40Shruti Semwal
... radical mastectomy. Patients with breast carcinoma other than primary adenocarcinoma such as lymphoma, sarcoma, stromal tumor and metastasis were excluded. Specimens submitted were examined grossly for tumor size, ...
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