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DAPI staining

The early stage of polyphosphate accumulation in saccharomyces cerevisiae: comparative study by extraction and DAPI staining

The early stage of polyphosphate accumulation in saccharomyces cerevisiae: comparative study by extraction and DAPI staining

... vivo DAPI staining is a simple method for vital observing of ...and DAPI staining (Figures 1 and 2) shows that staining character actually depends on PolyP ...Nevertheless, DAPI ...

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The IL 4/IL 13/Stat6 signalling pathway promotes luminal mammary epithelial cell development

The IL 4/IL 13/Stat6 signalling pathway promotes luminal mammary epithelial cell development

... B-I Immunohistochemistry IHC, right panels; left panels, merged with DAPI staining was performed on mammary gland sections from wild-type mice for: pStat6 in virgin mice B and mice at da[r] ...

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Non-benzoquinone geldanamycin analogs trigger various forms of death in human breast cancer cells

Non-benzoquinone geldanamycin analogs trigger various forms of death in human breast cancer cells

... cytometry, DAPI staining, ATP assay, electron microscopy, western blots, siRNAs transfection and immunofluorescence were used to determine the molecular mechanism of DHQ3- or 17-DR-induced different forms ...

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Antiosteogenic effect of arsenic trioxide, cholecalciferol, lovastatin or their combination in vitro

Antiosteogenic effect of arsenic trioxide, cholecalciferol, lovastatin or their combination in vitro

... NBT/BCIP staining solution (Thermo Fisher 34042) per well was added and incubated in the dark for 30 min to 1 h at room ...or DAPI staining to evaluate the percentage of AP positive over total live ...

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Antioxidant and anti-proliferative activity of different solvent extracts of Casuarina equisetifolia needles

Antioxidant and anti-proliferative activity of different solvent extracts of Casuarina equisetifolia needles

... Main findings: In vitro antioxidant activity of different solvent extracts of C. equisetifolia needles was studied by analyzing the total polyphenols, flavonoids, total antioxidant capacity and free radical scavenging ...

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Localization of Single- and Low-Copy Sequences on Tomato Synaptonemal Complex Spreads Using Fluorescence in Situ Hybridization (FISH)

Localization of Single- and Low-Copy Sequences on Tomato Synaptonemal Complex Spreads Using Fluorescence in Situ Hybridization (FISH)

... with DAPI only. Several SCs possess a re- gion where DAPI staining is not visible, ...a DAPI-negative band ...CPD staining of an early pachytene SC ...the DAPI-negative bands ...

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Role of Active Principles of Podophyllum hexandrum in Amelioration of Radiation Mediated Lung Injuries by  Reactive Oxygen/Nitrogen Species Reduction

Role of Active Principles of Podophyllum hexandrum in Amelioration of Radiation Mediated Lung Injuries by Reactive Oxygen/Nitrogen Species Reduction

... Images showing iNOS immunostaining in lung tissue sections A Nucleus DAPI staining in controls, B iNOS immunostaining in the controls showing bronchiole having large air spaces arrowhead[r] ...

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The origin of Rosenthal fibers and their contributions to astrocyte pathology in Alexander disease

The origin of Rosenthal fibers and their contributions to astrocyte pathology in Alexander disease

... and DAPI combined with immunohistochemical staining (c-d) in 1 year-old TG ...with DAPI counterstaining, which stains nuclei but not RFs, in combination with ...with DAPI, 2) Top astrocyte (in ...

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Convergent evolution of saccate body shapes in nematodes through distinct developmental mechanisms

Convergent evolution of saccate body shapes in nematodes through distinct developmental mechanisms

... our DAPI and live animal imaging suggest that proliferation of seam cells and the subsequent dorsoven- tral migration of daughter cells occur following ...Using DAPI staining, we found that the J3/J4 ...

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The chimeric multi-domain proteins mediating specific DNA transfer for hepatocellular carcinoma treatment

The chimeric multi-domain proteins mediating specific DNA transfer for hepatocellular carcinoma treatment

... Methods: We firstly constructed the multi-domain recombinant chimeric proteins based on recombinant proteins [G (yeast GAL4), NG (none GAL4), TG (GAL4 + Tat protein) and TNG (Tat protein)] and pUAS-Apoptin plasmid, and ...

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Potential therapeutic role of Tridham in human hepatocellular carcinoma cell line through induction of p53 independent apoptosis

Potential therapeutic role of Tridham in human hepatocellular carcinoma cell line through induction of p53 independent apoptosis

... cells. DAPI staining and agarose gel electrophoresis confirmed the induction of apoptosis by ...Immunofluorescent staining for Ki-67 showed a higher level of expression in untreated cells as compared ...

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Evaluation of Anticancer Activity of Alphonsea Sclerocarpa Crude Extract against HepG2 Cell Lines by Inducing Nuclear Morphology Changes and DNA Fragmentation

Evaluation of Anticancer Activity of Alphonsea Sclerocarpa Crude Extract against HepG2 Cell Lines by Inducing Nuclear Morphology Changes and DNA Fragmentation

... A DAPI staining assay was performed to identify the nuclear morphological ...of DAPI dye was added to the frozen cells and the mixture kept at 37°C for 30 minutes for staining protected from ...

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The impact of high salt exposure on cardiovascular development in the early chick embryo

The impact of high salt exposure on cardiovascular development in the early chick embryo

... Fig. 3. High-salt exposure limits ventricular wall development in E4.5 chick embryos. Fertilized chick eggs were exposed to 0.7% NaCl (control) and 300 mOsm l −1 NaCl (high salt) for 3 days as described above. Transverse ...

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Generation of Unfolded DNA in Human Neutrophils  Following Hypothermal Treatment

Generation of Unfolded DNA in Human Neutrophils Following Hypothermal Treatment

... Figure 2. Hypothermia/rewarming-induced DAPI-positive objects exhibited several features similar to PMA-stimulated NETs. (a) Human neutrophils were incubated in a culture dish containing a coverslip at 4˚C for 1 h ...

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10H-3,6-Diazaphenothiazine  induces G2/M phase cell cycle arrest and caspase-dependent apoptosis and inhibits cell invasion of A2780 ovarian carcinoma cells through the regulation of NF-κB and (BIRC6-XIAP) complexes

10<em>H</em>-3,6-Diazaphenothiazine induces G<sub>2</sub>/M phase cell cycle arrest and caspase-dependent apoptosis and inhibits cell invasion of A2780 ovarian carcinoma cells through the regulation of NF-&kappa;B and (BIRC6-XIAP) complexes

... an important role in the last stage of apoptosis as it acts as a marker to be engulfed by phagocytes. Therefore, PS can be used as a quantitative model to study apoptosis through counting the amount of Annexin V-FITC ...

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Original Article Sensitivities of periodic acid-Schiff staining, Grocott’s silver staining and calcofluor white staining in the diagnosis of human sporotrichosis

Original Article Sensitivities of periodic acid-Schiff staining, Grocott’s silver staining and calcofluor white staining in the diagnosis of human sporotrichosis

... sue on silver staining, but negative result was found on fluorescence staining. Moreover, the detection of PAS+GSS+CFW was only 77%, which was comparable to that of CFW (P > 0.05) (Table 2). On PAS, ...

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Pulmonary exposure to single-walled carbon nanotubes does not affect the early immune response against Toxoplasma gondii

Pulmonary exposure to single-walled carbon nanotubes does not affect the early immune response against Toxoplasma gondii

... normal horse serum (Vector Labs, Burlingame, CA, USA) to block nonspecific reactivity. Sections were incubated with rabbit-anti-mouse MPO (ab9535, AbCam, Cam- bridge, UK; 1:100, 4°C overnight) and with a peroxidase ...

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Electroacupuncture attenuated cerebral ischemic injury and neuroinflammation through α7nAChR-mediated inhibition of NLRP3 inflammasome in stroke rats

Electroacupuncture attenuated cerebral ischemic injury and neuroinflammation through α7nAChR-mediated inhibition of NLRP3 inflammasome in stroke rats

... immunoflourescent staining. The infarct size, neurological deficit score, TUNEL staining and the expression of proinflammatory factors or anti-inflammatory cytokines were evaluated at 72 h after reperfusion ...

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Equine Myxovirus Resistance Protein 2 Restricts Lentiviral Replication by Blocking Nuclear Uptake of Capsid Protein

Equine Myxovirus Resistance Protein 2 Restricts Lentiviral Replication by Blocking Nuclear Uptake of Capsid Protein

... FIG 3 Equine Mx2 inhibits HIV-1 infection and nuclear uptake of HIV-1 cDNA. (A) HEK293T cells were transfected with 1 ␮ g plasmids expressing either huMxA, huMxB, rhTRIM5 ␣ , or eqMx2. huMxB and rhTRIM5 ␣ served as ...

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Cdc42 regulates Cdc42EP3 function in cancer-associated fibroblasts.

Cdc42 regulates Cdc42EP3 function in cancer-associated fibroblasts.

... (blue) staining of cancer-associated fi broblasts (CAFs) stably expressing Cdc42EP3-GFP following transfection with empty vector, myc-Cdc42-N17 or ...(blue) staining of cancer-associated fibroblasts (CAFs) ...

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