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DNA-binding specificity

The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.

The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.

... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ...

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The Basis for Evolution of DNA-Binding Specificity of the Aft1 Transcription Factor in Yeasts

The Basis for Evolution of DNA-Binding Specificity of the Aft1 Transcription Factor in Yeasts

... To confirm the results of the bioinformatic analysis described above, we tested the ability of LtAft, the L. thermotolerans ortholog of Aft1 and Klaft, to bind the TGCACCC sequence. We choose L. thermotolerans because the ...

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Herpes Simplex Virus Processivity Factor UL42 Imparts Increased DNA-Binding Specificity to the Viral DNA Polymerase and Decreased Dissociation from Primer-Template without Reducing the Elongation Rate

Herpes Simplex Virus Processivity Factor UL42 Imparts Increased DNA-Binding Specificity to the Viral DNA Polymerase and Decreased Dissociation from Primer-Template without Reducing the Elongation Rate

... During DNA replication, long-chain DNA synthesis on the leading strand requires processivity factors to overcome the tendency of DNA polymerase to dissociate from the template after each catalytic ...

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DNA-Binding Specificity of the IDI-4 Basic Leucine Zipper Factor of Podospora anserina Defined by Systematic Evolution of Ligands by Exponential Enrichment (SELEX)

DNA-Binding Specificity of the IDI-4 Basic Leucine Zipper Factor of Podospora anserina Defined by Systematic Evolution of Ligands by Exponential Enrichment (SELEX)

... consensus binding sequence corre- sponds to the ATGANTCAT ...the binding sequence of the yeast bZIP GCN4 factor (8, 18) and of its orthologs in filamentous ...in DNA contacts in GCN4 are strictly ...

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In vitro transcriptional activation, dimerization, and DNA-binding specificity of the Epstein-Barr virus Zta protein.

In vitro transcriptional activation, dimerization, and DNA-binding specificity of the Epstein-Barr virus Zta protein.

... In a previous study, Zta was shown to footprint over six sites in the BHLF1 upstream control region and to bind to an oligonucleotide containing the collagenase AP-1 site but not to seve[r] ...

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Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity.

Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity.

... 5 0022-538X/88/051598-08$02.00/0 Copyright © 1988, American Society for Microbiology Mutations in the Phosphorylation Sites of Simian Virus 40 SV40 T Antigen Alter Its Origin DNA-Binding[r] ...

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Using mutual information to reprogram DNA specificity of LAGLIDADG endonucleases

Using mutual information to reprogram DNA specificity of LAGLIDADG endonucleases

... nt specificity at obvious aa-nt hydrogen contacts, however, they had difficulty in efforts to reprogram extensive protein-DNA interfaces (Ashworth et ...the DNA-binding specificity of ...

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Interaction of DNA with groove binding ligands

Interaction of DNA with groove binding ligands

... groove binding molecules usually have several simple aromatic rings (benzene, pyrrole etc) connected by bonds with torsional freedom that allow appropriate twisting to enable a good shape fit into the minor ...

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Molecular binding of formaldehyde to DNA and proteins

Molecular binding of formaldehyde to DNA and proteins

... 1.7.3 Challenge 3: Differentiating endogenous and exogenous formaldehyde As we mentioned before, formaldehyde is a ubiquitous environment pollutant. Of equal importance, formaldehyde is an essential metabolic ...

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Adenovirus Type 5 DNA Binding Protein Stimulates Binding of DNA Polymerase to the Replication Origin

Adenovirus Type 5 DNA Binding Protein Stimulates Binding of DNA Polymerase to the Replication Origin

... (Ad) DNA-binding protein (DBP) is essential for the elongation phase of Ad DNA replication by unwinding the template in an ATP-independent fashion, employing its capacity to form ...Ad DNA ...

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RNA Bind-n-Seq: Quantitative Assessment of the Sequence and Structural Binding Specificity of RNA Binding Proteins

RNA Bind-n-Seq: Quantitative Assessment of the Sequence and Structural Binding Specificity of RNA Binding Proteins

... RNA-protein binding followed by microarray analysis, enabling high-throughput identification of RNA binding motifs (Ray et ...RNA binding, and RNAcompete does not yield K d ...one-step binding ...

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DNA-binding activity of papillomavirus proteins.

DNA-binding activity of papillomavirus proteins.

... Further characterization of the DNA-binding activity of these E2 proteins at 50 mM NaCl, pH 7.0, demonstrated that all [32P]-labeled DNAs, including pBR322, were bound with equal efficie[r] ...

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DNA Binding Polyamides in Biological Systems

DNA Binding Polyamides in Biological Systems

... Minor -groove binding transcription factors, such as TBP Figure 1.3, LEF-l, and TAX, have been excellent targets for polyamide inhibition, resulting in inhibition of RNA polymerase II tr[r] ...

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Electrical Detection of DNA Binding Proteins

Electrical Detection of DNA Binding Proteins

... I want to give a great deal of credit to Mike Hill at Occidental College. Without Mike, none of the DNA electrochemistry experiments described in this thesis would be possible. He has been an amazing resource, and ...

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Multimerization of Hepatitis Delta Antigen Is a Critical Determinant of RNA Binding Specificity

Multimerization of Hepatitis Delta Antigen Is a Critical Determinant of RNA Binding Specificity

... whether binding is pre- ceded by multimer dissociation, we compared RNP complexes formed by HDAg-160, HDAg-145, an additional truncated form of the protein, and a mixture of these two truncated forms of ...

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HPV 9G DNA Chip: 100% Clinical Sensitivity and Specificity

HPV 9G DNA Chip: 100% Clinical Sensitivity and Specificity

... 9G DNA chips obtained by the 9G DNA chip technology showed excellent performance in terms of the SBR of 200 and 100% target-specific ...9G DNA chip test and the commercial HPV DNA chip test ...

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Receptor binding specificity and origin of 2009 H1N1 pandemic influenza virus

Receptor binding specificity and origin of 2009 H1N1 pandemic influenza virus

... primary binding specificity of both groups was human type re- ceptor at CIS frequency ...secondary binding frequencies, with the HA proteins from Singapore having ...

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Specificity of cleavage in replicative-form DNA of bovine herpesvirus 1.

Specificity of cleavage in replicative-form DNA of bovine herpesvirus 1.

... In contrast, the putative cleavage site in the fusion of replicative-form DNA in BHV-1 is composed of a unique arrangement of sequence elements compared with the internal junction IR-UL-[r] ...

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Specificity of initiation of plus-strand DNA by Rous sarcoma virus.

Specificity of initiation of plus-strand DNA by Rous sarcoma virus.

... a Published sequence of viral RNA 20; b deduced 5' end of strong-stop plus DNA, as synthesized in the endogenous reaction; c sequence of the RNA primer attached to strong-stop plus DNA f[r] ...

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Inhibition of p53 expression modifies the specificity of chromatin binding by the androgen receptor

Inhibition of p53 expression modifies the specificity of chromatin binding by the androgen receptor

... the specificity of chromatin binding by ...on binding of AR and FoxA1 to DNA, in lysates of androgen-deprived LNCaP and LNsip53 cells treated with 10 nM of ...

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