DNA extraction, ITS amplification and sequencing
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
... Illumina sequencing platforms (once sequencing adaptors are ...of DNA if concentrations of the target DNA are ...amplify DNA from the target ...target DNA regions identified to ...
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Integrated DNA extraction and amplification on a microfluidic device
... system. DNA extraction and pre-concentration was performed ‘off-chip’ using commercial ...The DNA sample was then manually injected into a 160 nl PCR chamber along with all the necessary reagents to ...
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illustra TempliPhi DNA Sequencing Template Amplification Kit
... TempliPhi DNA Sequencing Template Amplification Kit consists primarily of two components, denature buffer and TempliPhi ...for amplification, and is adjusted to a pH that supports DNA ...
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Methods for microbial DNA extraction from soil for PCR amplification
... of DNA-based techniques can overcome this limitation by allowing the fate of particular genes or organisms to be monitored directly in environmental ...extract DNA from soil and sediment initially used ...
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Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification
... Finally, as we have already mentioned, the main reason for optimising the DNA extraction protocol was to analyse parasite infection in Lutzomyia spp. by PCR amplification. In this sense, it is ...
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Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results
... for DNA extraction, two library preparation kits and two NGS Illumina sequencing ...similar sequencing and assembly param- eters statistics with reference to sequencing outputs, in ...
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Detection of Chlamydia trachomatis DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems
... Gel Extraction Kit ® + CTAB gave the highest sensitiv- ity to detect ...EB DNA from SF in combination with the ...EB DNA were achieved using alkaline lysis, QIAmp Tissue Kit ® and QIAmp Stool Kit ® ...
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Simple and quick method of DNA extraction from different parts of plant for PCR amplification
... the DNA molecular marker based genetic ...genomic DNA is the prerequisite for molecular biology research. An efficient DNA extraction method should be simple, rapid and affordable to provide ...
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16S rRNA gene sequencing of mock microbial populations-impact of DNA extraction method, primer choice and sequencing platform
... and sequencing platform on results, we next aimed to determine the effects of DNA extraction procedures on the sequencing results ...for sequencing results from both platforms and all ...
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Comprehensive DNA methylation analysis of tissue of origin of plasma cell-free DNA by methylated CpG tandem amplification and sequencing (MCTA-Seq)
... DNA extraction and MCTA-Seq library preparation Commercialized genomic DNAs for normal tissues, in- cluding the lung (n = 2), stomach (n = 2), colon (n = 2), kidney (n = 2), pancreas (n = 2), muscle (n = ...
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A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction
... isothermal amplification assay for rapid detection of hepatitis B virus in human blood at 65 °C in less than 60 min and each test costs ...with DNA extraction revealed the ability to detect HBV ...
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Grapevine yellows: Comparison of different procedures for DNA extraction and amplification with PCR for routine diagnosis of phytoplasmas in grapevine
... plant DNA extraction and 4 procedures for amplifica- tion in polymerase chain reaction of phytoplasma DNA were examined in parallel using the same plant tissues infected with ...The DNA ...
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AMPLIFICATION AND SEQUENCING OF MITOCHONDRIAL DNA (HVR I) EXTRACTED FROM 1200 YEARS OLD HUMAN BONE SPECIMEN
... Ancient DNA help in better understanding the evolution and migration pattern of humans throughout the ...ancient DNA from human bone samples provided by the Anthropological survey of India collected from ...
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Detection of rubella virus gene sequences by enzymatic amplification and direct sequencing of amplified DNA
... target cDNA reverse transcribed from RNA extracted from purified stock RV (100 ,ul) (lanes 1 and 2), total cytoplasmic RNA (1 pug) extracted from RV-infected Vero cells at 36 h (lanes 3 [r] ...
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Combination of PURE DNA extraction and LAMP DNA amplification methods for accurate malaria diagnosis on dried blood spots
... isothermal amplification (LAMP) method is a DNA amplification tool in which the DNA amplification can be achieved by incubation at a stable ...
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Impact of Contaminating DNA in Whole Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis
... There are potential methods to account for contaminant DNA during the metag- enomics data analysis. We have observed that reads from contaminant DNA often result from the large-scale amplification of small ...
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DNA sequence variation within maize and melon: observations from polymerase chain reaction amplification and direct sequencing.
... lower. The reason for this observed difference in genetic variation is not well understood. There ap- pears to be no correlation between this disparity and genome size [r] ...
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COLD-PCR Amplification of Bisulfite-Converted DNA Allows the Enrichment and Sequencing of Rare Un-Methylated Genomic Regions
... of DNA is evident in a range of human diseases including cancer and ...un-methylated DNA within methylated samples can be useful in several ...converted DNA, fast-COLD-MS- PCR enriches un-methylated ...
Identification of Leishmania spp by Molecular Amplification and DNA Sequencing Analysis of a Fragment of rRNA Internal Transcribed Spacer 2
... the ITS region: the rRNA internal spacers are subject to less evolutionary pressure and show more se- quence divergence than the coding regions and have been proposed as targets for molecular typing (1, 6, ...
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Certified DNA Reference Materials to Compare HER2 Gene Amplification Measurements Using Next-Generation Sequencing Methods
... and sequencing primers from the sequences. The trimmed sequences were mapped to human genome reference hg19 using the Burrows-Wheeler Aligner software version 0.6.2 aln 32 and sample mode in default settings. The ...
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