DNA Sequencing and PeR-Based Amplification
illustra TempliPhi DNA Sequencing Template Amplification Kit
... TempliPhi DNA Sequencing Template Amplification Kit consists primarily of two components, denature buffer and TempliPhi ...for amplification, and is adjusted to a pH that supports DNA ...
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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
... article bau061. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Bolchacova E, Voigt K, Crous PW, Miller AN, Wingfield MJ, Aime MC, An KD, Bai FY, Barreto RW, Begerow D, Bergeron MJ, Blackwell ...
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Detection of rubella virus gene sequences by enzymatic amplification and direct sequencing of amplified DNA
... target cDNA reverse transcribed from RNA extracted from purified stock RV (100 ,ul) (lanes 1 and 2), total cytoplasmic RNA (1 pug) extracted from RV-infected Vero cells at 36 h (lanes 3 [r] ...
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Microfluidic DNA amplification - a review
... single DNA molecule and single-cell amplification, and can eliminate the preference for amplifying short fragments and the production of short chimeric molecules associated with single-phase ...for ...
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Impact of Contaminating DNA in Whole Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis
... There are potential methods to account for contaminant DNA during the metag- enomics data analysis. We have observed that reads from contaminant DNA often result from the large-scale amplification of small ...
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Procedures For DNA Sequencing
... For high through put needs samples may be submitted in 96-well plates. The round or V-bottom plates (any manufacturer is acceptable) must be well sealed and labeled. The plate positions must be filled by columns, e.g. ...
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IDTutorial: DNA Sequencing
... electrophoresis was a well established technique in analytical chemistry in the late 1980’s. Capillaries are small, a 50µm inner diameter, and they dissipate heat very efficiently due to their high surface area to volume ...
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An Overview of DNA Sequencing
... Library prep, amplification and sequencing: 2-4 days Single sample preparation from bacterial to human genomic DNA Single amplification per genome with no cloning or cloning artifacts Pi[r] ...
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Evolution of DNA Sequencing
... allows DNA fragments separation based on their ...rapid DNA fragment separation, it is the ease of automation that has resulted in its induction in sequencing instruments, in place of slab gel ...
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Comprehensive DNA methylation analysis of tissue of origin of plasma cell-free DNA by methylated CpG tandem amplification and sequencing (MCTA-Seq)
... of amplification (using primers QP1 (5′-AATG ATACGGCGACCACCGA-3′) and QP2 (5′-CAAGCAG AAGACGGCATACGA-3′)) and gel purification are usu- ally needed to clean up the primer dimers and obtain enough materials for ...
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MAKING MORE SENSITIVE DNA SENSORS USING GOLD NANOPARTICLES AND DNA-BASED AMPLIFICATION NETWORKS
... Combinatorial DNA Hybridizations. Each lane contains some combination of the DNA strands hairpin (H), linker/signal (L/S), signal (S), transducer (Trn), and the biomolecule adenosine ...the ...
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A closed tube format for amplification and detection of DNA based on energy transfer
... The method described in this paper can be applied to any diagnostic procedure where the presence of the target nucleic acid is to be detected either qualitatively or quantitatively. It may be applied to the detection of ...
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A School-based Chlamydia Control Program Using DNA Amplification Technology
... We found that school-based chlamydia screening and treatment are feasible, acceptable, and relatively inexpensive, and has a high yield. A limitation on the interpretation of our data is that only 59% of the ...
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Classification of human cancers based on DNA copy number amplification modeling
... to DNA double-strand breaks and enable ...of amplification pre- dict that DNA double-strand breaks must occur at the ends of the amplified chromosomal ...of amplification patterns were ...
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A School-based Chlamydia Control Program Using DNA Amplification Technology
... METHODS All youth in the school were eligible to participate if they had parental consent to receive clinical services in the school-based clinic or if the parents had signed a specific consent allowing the ...
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DNA sequencing. DNA sequencing by the chemical method of Maxam and Gilbert (PNAS, 1977)
... disegnato per ibridarsi con la regione lontana circa 300 basi a valle del sito di innesco del primo ...legame per l’innesco, si sintetizza un altro oligonucleotide e si determina la sequenza ...
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Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification
... sequence-based amplification (NASBA)-based HPV detection using HPV DNA plasmids (HPV type 16 [HPV16], HPV18, HPV31, HPV33, and HPV45) and nucleic acid extracts of several cell lines, which ...
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DNA sequence variation within maize and melon: observations from polymerase chain reaction amplification and direct sequencing.
... lower. The reason for this observed difference in genetic variation is not well understood. There ap- pears to be no correlation between this disparity and genome size [r] ...
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COLD-PCR Amplification of Bisulfite-Converted DNA Allows the Enrichment and Sequencing of Rare Un-Methylated Genomic Regions
... of DNA is evident in a range of human diseases including cancer and ...un-methylated DNA within methylated samples can be useful in several ...converted DNA, fast-COLD-MS- PCR enriches un-methylated ...
Identification of Leishmania spp by Molecular Amplification and DNA Sequencing Analysis of a Fragment of rRNA Internal Transcribed Spacer 2
... Culture and isoenzyme analysis. The culture tubes contained Novy-MacNeal- Nicolle agar with 10% defibrinated rabbit blood and an overlay of RPMI medium with 15% fetal bovine serum. The tubes were incubated at 25°C; ...
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