Double- and Single-Strand DNA
The role of telomeres and telomerase in hematologic malignancies and hematopoietic stem cell transplantation
... ss DNA: Single-strand DNA; ds DNA: Double-strand DNA; TRF1: Telomeric repeat-binding factors 1; TRF2: Telomeric repeat-binding factors 2; TIN2: TRF1-interacting ...
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Evaluation of DNA Integrity of Cryopreserved Boer Goat (Capra hircus) Sperm Using Comet Assay at Various pH Conditions
... that double-strand DNA breaks (neutral comet assay) were significantly higher than single-strand DNA breaks (alkaline comet ...higher double-strand DNA ...
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Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania
... No single current gRNA design program tested can accurately predict gRNA activity in Leishmania, and the gene-targeting efficiency for the same gRNA construct can vary greatly in different Leishmania ...a ...
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Effects of DNA Double-Strand and Single-Strand Breaks on Intrachromosomal Recombination Events in Cell-Cycle-Arrested Yeast Cells
... site-specific DNA SSB on DEL recombina- tion frequencies: The gene II protein (gIIp) of the fila- mentous coliphages (f1, fd and M13) is a multifunc- tional protein required for DNA ...initiates DNA ...
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Replication Protein A Is Required for Meiotic Recombination in Saccharomyces cerevisiae
... transient DNA double-stranded breaks ...3⬘ single-stranded DNA ends that serve to channel DSBs into the RAD52 recombinational repair ...the strand exchange ...the DNA-binding ...
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Predicting double strand DNA breaks using epigenome marks or DNA at kilobase resolution
... DSBs in situ and it can directly map them at single- nucleotide resolution. DSBCapture peaks were called with less than 1-kb resolution (median size of 391 bases). The DSBCapture peaks obtained from two biological ...
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Differential Effects of DNA Double-Strand Break Repair Pathways on Single-Strand and Self-Complementary Adeno-Associated Virus Vector Genomes
... complementary strand synthesis, they can be used to specifically test the con- tributions of DNA repair factors to recombination indepen- dent of dsDNA ...different DNA repair-deficient cell lines ...
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Covalently closed circular viral DNA formed from two types of linear DNA in woodchuck hepatitis virus-infected liver.
... large single-stranded region at the 5 9 end of the minus ...circular DNA by extension of the 3 9 end of the minus strand to the 5 9 end of the plus strand at DR2 ...minus strand from ...
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Novel treatment strategies in triple-negative breast cancer: specific role of poly(adenosine diphosphate-ribose) polymerase inhibition
... as single agents, in tumors harboring defects in double-strand DNA-break repair, the best studied being ovarian and breast cancers with BRCA germ-line mutations, in which they have induced ...
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Capture of DNA Sequences at Double-Strand Breaks in Mammalian Chromosomes
... Inserted into the tk gene is a 24-bp oligonucleotide (hatched electroporation cells were fed with DMEM supplemented with segment) containing the 18-bp recognition site for yeast endo- hypoxanthine/aminopterin/thymidine ...
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Deoxyribonucleic acid strandedness Partial characterization of the antigenic regions binding antibodies in lupus erythematosus serum
... labeled DNA prepared from mammalian cells by the Marmur technique is a pure preparation of nucleic acid that is composed essentially of two populations of ...primarily double- standed nucleic acid, while ...
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Strand Invasion and DNA Synthesis From the Two 3′ Ends of a Double-Strand Break in Mammalian Cells
... independent strand invasion and copying ...initiate strand invasion and DNA synthesis, after which it would be ejected from the endogenous ...initiate strand invasion and DNA synthesis, ...
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Studies on DNA Topology and DNA-Amyloid Beta (Aβ) peptides interaction in relevance to Alzheimer's Disease
... SAD DNA were fragmented as evidenced by their smear migration ...SAD DNA as both of them have smeared pattern, EtBr staining is faint for SAD ...to DNA isolated from other regions of the brain, ...
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DNA synthesis errors associated with double-strand-break repair.
... In contrast to the spontaneous revertants that were found equally on both chromosomes, it is remarkable that nearly all the revertants were on the chromosome that had th[r] ...
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Molecular Mechanisms of H. pylori Induced DNA Double-Strand Breaks
... H. pylori infection induces DNA damage on gastric epithelial cells [63].Contact dependent interactions between H. pylori bacteria and gastric epithelial cells activate intracellular signaling events that have ...
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Dissecting The Functions Of Atr In Replication Fork Stability
... of DNA synthesis, replisome disassembly and decatenation (Dewar and Walter, ...which DNA synthesis is completed is also under ...the single-stranded gap remaining between the leading stand and the ...
204
Capture of Extranuclear DNA at Fission Yeast Double-Strand Breaks
... of DNA double-strand breaks (DSBs) is necessary for the maintenance of genomic integ- ...mitochondrial DNA (mtDNA) at high ...microsatellite DNA from higher eukaryotes was ...
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Hypersensitivity of ataxia telangiectasia cells to DNA double strand breaks
... of DNA strand breaks in intact cells can be achieved by use of following m ethods: 1) classical analysis of a DNA profile w ith different molecular w eights on an alkaline or a neutral sucrose ...
305
BCHM3050 Lecture 12 F14.ppt
... of DNA Topoisomerases – prevent supercoiling & tangling of DNA during unwinding; bind ahead of the replication fork, nick during unwinding; bind ahead of the replication fork, nick supercoiling ...
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Original Article Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan
... of DNA double strand break, showed that its expression was significantly increased in foci of BilIN, IPNB and invasive carcinoma as well as in non-neoplastic biliary epithelial cells of the printing ...
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