Double knockout mice
Atherosclerosis, inflammation and lipoprotein glomerulopathy in kidneys of apoE-/-/LDL-/- double knockout mice
... Coexistence of hypercholesterolemia and hypertension increased Vasa vasorum density in rats [22], this is of particular interest, because our animals showed severe atherosclerotic lesions in the renal artery and ...
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Hepatic caveolin 1 is enhanced in Cyp27A1/ApoE double knockout mice
... ) double knockout mice (DKO) fed a western diet failed to develop ...DKO mice were fed a western diet for 2 ...using mice plasma as ...DKO mice and negatively correlated with ...
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Caspase dependent secondary lens fiber cell disintegration inα A /αB crystallin double knockout mice
... To strengthen the caspase activity data from wild-type and DKO mouse lens extracts, we employed immunofluorescence microscopy of frozen sections stained with anti-active caspase 3 or anti-caspase 6 antibodies (Fig. 4). ...
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Original Article Changes in spontaneous thrombolytic activity during progression of atherosclerosis in Apo and LDLR double knockout mice
... (C57Bl6 mice) a high-fat diet for a total of 22 weeks and evaluated them at 14 and 22 weeks. The amount of atherosclerosis was estimated as the ratio of the atherosclerotic to total aortic intimal area. In ...
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Molecular signatures of neurodegeneration in the cortex of PS1/PS2 double knockout mice
... Presenilins associate with Aph1, Pen2 and Nicastrin in a large complex that mediates intramembranous, " γ -secre- tase" [1] proteolysis of a variety of type-I membrane pro- teins, including Notch, APP, ...
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Lung fluid transport in aquaporin 5 knockout mice
... null mice showed a biphasic behavior consisting of a small, prompt signal deflection (Figure 2, leftward arrows) followed by a slower change in the fluores- cence making up the majority of the signal ampli- ...
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Pathogenesis of Neonatal Herpes Simplex 2 Disease in a Mouse Model Is Dependent on Entry Receptor Expression and Route of Inoculation
... in mice differs based on availability of the principal entry receptors herpesvirus entry mediator (HVEM) and nectin-1 in a manner dependent upon route of ...adult mice, nectin-1 is a major mediator of ...
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Ephrin-A2 and ephrin-A5 guide contralateral targeting but not topographic mapping of ventral cochlear nucleus axons
... type mice numerous calyces are labeled in ...type mice. d In ephrin-A2/A5 double knockout mice, VCN axons terminated in ...ephrin-A2/A5 double knockout mice also ...
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The LEM Domain Proteins Emerin and LAP2α Are Dispensable for Human Immunodeficiency Virus Type 1 and Murine Leukemia Virus Infections
... ⫺/⫺ knockout and Emd ⫺/⫺ Tmpo ⫺/⫺ double knockout mice compared to wild-type littermate controls were ...of mice was injected with 2 ml of 3% thioglycolate in ...
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Generation of Smurf2 Conditional Knockout Mice
... carcinogenesis. Mice with conventional knockout of Smurf1 or Smurf2 alleles are viable, but conventional Smurf1 and Smurf2 double knockout mice were early embryonic ...flox/flox ...
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Effects of altered ephrin-A5 and EphA4/EphA7 expression on tumor growth in a medulloblastoma mouse model
... whether knockout of ephrin-A5 (present on granule cell pre- cursors [3]) and its receptors, EphA4/EphA7 (present on Purkinje cells [3]), would interfere with the spatio- temporal development of tumors in this ...
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Distinct Roles of Synapsin I and Synapsin II during Neuronal Development
... apses in hippocampal neurons from wild-type mice and from synapsin II and synapsin I/II double knockout mice. Note the absence of synap- tophysin immunoreactive spots at 4 days in neuron[r] ...
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A genetic model of substrate deprivation therapy for a glycosphingolipid storage disorder
... the double knockout mice did not completely restore neurologic function to control ...the double knockout mice began to exhibit ataxia that pro- gressively worsened over ...of ...
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Regulation of obesity and insulin resistance by hypoxia-inducible factors
... α double-knockout mice and, intriguingly, found that double-knockout mice appear to recapitulate virtually all phenotypes that HIF-1 α knock- out mice exhibit: improved ...
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Generation of knockout mouse models of cyclin-dependent kinase inhibitors by engineered nuclease-mediated genome editing
... Kip1 knockout mice via clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 ...cell-based knockout mice, our mouse models are free from selectable markers or ...
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Multidrug resistance protein 1 protects the choroid plexus epithelium and contributes to the blood cerebrospinal fluid barrier
... homemade double can- nula made of 19-gauge and 23-gauge needles with 4-mm extending tips of fused silica (outer diameter ...This double cannula was subse- quently lowered through the brain tissue into the ...
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Decreased thyroid follicle size in dwarf mice may suggest the role of growth hormone signaling in thyroid growth regulation
... In agreement with the above, experimental evidence suggests that reduced GH signaling may contribute to lifespan extension. Therefore, several types of laboratory animals with disruption of somatotrophic axis are used in ...
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VAV1-Cre mediated hematopoietic deletion of CBL and CBL-B leads to JMML-like aggressive early-neonatal myeloproliferative disease
... Interestingly, compared to the rapid lethality of the donor mice (Figure 1D), the disease progression in the transplanted mice was slower (Figure 5B). Although severe MPD was seen by the time of the ...
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c Jun N terminal kinase is required for metalloproteinase expression and joint destruction in inflammatory arthritis
... JNK inhibition also decreased the induction of AP-1 DNA-binding activity in synoviocytes, which is prima- rily dependent on new Jun and Fos protein synthesis in synovial fibroblasts. Of particular interest, SP600125 ...
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Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen
... live mice. With this model, we evaluated thrombus growth in mice lacking von Willebrand factor (vWF) and fibrinogen (Fg), the two key ligands known to mediate platelet adhesion and ...–/– mice, ...
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