E6-AP
Growth Suppression Induced by Downregulation of E6-AP Expression in Human Papillomavirus-Positive Cancer Cell Lines Depends on p53
... that E6-AP is required for both p53 degradation and the viability of HPV-positive cell lines (4, 12, 15, 16, ...of E6-AP to facilitate p53 degradation in the presence of E6 is essential ...
5
Mutational analysis of human papillomavirus type 16 E6 demonstrates that p53 degradation is necessary for immortalization of mammary epithelial cells.
... 16 E6. While the mechanisms by which E6 induces p53 degradation in MECs are not defined, one likely pathway, reflected in the in vitro assay, is through complex formation with E6-AP with the ...
6
Transformation by bovine papillomavirus type 1 E6 is independent of transcriptional activation by E6.
... BPV-1 E6 and E7 regions were modified by oligonucleotide-mediated site-directed mutagenesis to eliminate a nonconserved stop codon at nucleotide (nt) 48 (TAG-to-TAC mutation), to introduce an NcoI site at amino ...
5
High-risk human papillomavirus E6 protein has two distinct binding sites within p53, of which only one determines degradation.
... for E6 to associate with wild-type p53 (20, 21), in amounts sufficient to saturate any E6 ...of E6-AP enhanced the binding of E6 to GST-p53 but not to the mutant ...of ...
8
Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1
... of AP-1 complex was present in the crude cyto- solic and membrane-bound fractions, as judged by immuno- precipitation of AP-1 ...wild-type E6 or mutant E6 does not change AP-1 levels or ...
7
Progesterone receptors animal models and cell signaling in breast cancer: Role of steroid receptor coactivators and corepressors of progesterone receptors in breast cancer
... AF = activation function domain; CBP = CREB-binding protein; E6-AP = E6-associated protein; ER = estrogen receptor; N-CoR = nuclear receptor corepressor; PR = progesterone receptor; RPF1[r] ...
5
Human Papillomavirus Type 16 E6-Induced Degradation of E6TP1 Correlates with Its Ability To Immortalize Human Mammary Epithelial Cells
... HPV16 E6 mutants were cotransfected into 293T cells by the calcium phosphate method (33), cells were lysed after 48 h, and the lysates were subjected to immunoblotting using a rabbit anti-E6TP1 anti- ...of ...
8
Differential localization of HPV16 E6 splice products with E6 associated protein
... truncated E6 proteins raised the possibility that truncated forms of E6 might be present in such diminishingly small quanti- ties that they may not have significant function ...of E6*I and ...
6
AP Poetry_Essays_with_Poems.doc
... Prompt: Read carefully the following poem by Marilyn Nelson Waniek. Then write an essay analyzing how Waniek uses literary techniques to develop the complex meanings that the speaker [r] ...
48
AP-LabReview.pptx
... Using measurements of dissolved oxygen concentration to determine primary productivity, design a controlled experiment to test the. hypothesis that primary productivity is affected by e[r] ...
72
In vitro biological activities of the E6 and E7 genes vary among human papillomaviruses of different oncogenic potential.
... Our data obtained with the keratinocyte immortalization assay also show that E6 from the low-risk HPV types is less active than E6 from the high-risk types, since E6 from HPV-6 or HPV-11[r] ...
7
Detection of Human Papilloma Virus (HPV) Type 16 and 18 using Immunohistochemistry among Sudanese Patients with Prostate Cancer
... The Human Papillomavirus (HPV) E6 protein is one of three oncoproteins encoded by the virus. It has long been recognized as a potent oncogene and is intimately associated with the events that result in the ...
12
E6^E7, a Novel Splice Isoform Protein of Human Papillomavirus 16, Stabilizes Viral E6 and E7 Oncoproteins via HSP90 and GRP78
... BPV-1 E6 ORF could be spliced to the E7 ORF in frame, leading to the production of a novel E6^E7 mRNA to encode a hypothetical E6^E7 fusion protein with a size of 183 aa residues (71 aa residues from ...
15
The ability of human papillomavirus E6 proteins to target p53 for degradation in vivo correlates with their ability to abrogate actinomycin D-induced growth arrest.
... To determine whether the ability of E6 to interact with p53 leads to a disruption of cell cycle control, mutated E6 proteins were tested for p53 binding and p53 degradation targeting in [r] ...
8
E6AP-Dependent Degradation of DLG4/PSD95 by High-Risk Human Papillomavirus Type 18 E6 Protein
... examine the biological effect(s) of exogenous DLG4 expression in CaSki cells. As carboxy-terminal PDZ domain-binding motif- dependent transformation of 3Y1 cells is evident only after cells become confluent (16), we ...
11
Human Papillomavirus E6 Triggers Upregulation of the Antiviral and Cancer Genomic DNA Deaminase APOBEC3B
... of E6. Transduction experiments showed that the E6 oncoprotein alone was sufficient to cause A3B upregulation, and a panel of high-risk E6 proteins triggered higher A3B levels than did a panel of ...
8
Interaction of the Papillomavirus E8∧E2C Protein with the Cellular CHD6 Protein Contributes to Transcriptional Repression
... the E6 and E7 oncogenes of high-risk human papillomaviruses (HPV) is controlled by cellular transcription factors and by viral E2 and E8∧E2C proteins, which are both derived from the HPV E2 ...HPV E6/E7 ...
11
Glycogene expression profiles based on microarray data from cervical carcinoma HeLa cells with partially silenced E6 and E7 HPV oncogenes
... the E6, E7, POFUT1 , XXYLT1 , DPY19L1, ALG14, UGT8 , PIGV , and GALNT1 genes were optimal, standard curves were constructed with the following concentrations: 10, 1, ...the E6, E7, POFUT1, XXYLT1 , DPY19L1 ...
12
Involvement of Nuclear Export in Human Papillomavirus Type 18 E6-Mediated Ubiquitination and Degradation of p53
... HPV-18 E6-mediated p53 nuclear export but is not essential for p53 ...HPV-18 E6 is unable to efficiently shuttle p53(NES-) to the ...HPV-18 E6 or Mdm2 expression vectors, as ...
11
Papillomavirus Type 16 Oncogenes Downregulate Expression of Interferon-Responsive Genes and Upregulate Proliferation-Associated and NF-κB-Responsive Genes in Cervical Keratinocytes
... that E6-mediated degradation of p53 contributes to this response but is not exclusively respon- ...that E6 decreased constitutive expression of IFN-␣ and - mRNAs and diminished the intracellular level of ...
14