Endotoxin Assay: The Chromogenic Limulus Amebocyte Lysate
Single step, chromogenic Limulus amebocyte lysate assay for endotoxin
... With a multiple- reagent chromogenic method in which the enzyme is acti- vated by LAL in the first step and color is developed after addition of the buffer and the substrate in the secon[r] ...
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New microassay for quantitation of endotoxin using Limulus amebocyte lysate combined with enzyme linked immunosorbent assay
... The Limulus amebocyte lysate (LAL) test, developed from the pioneer studies by Bang and Levin (3, 17) on the blood coagulation system in Limulus, is by far the most sensitive and specifi[r] ...
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Determination of endotoxin in injectable antibiotic preparations by the chromogenic assay method using a Limulus reagent (Tachypleus hemocyte lysate) and a chromogenic substrate
... In other antibiotics with moderate or strong inhibition, endotoxin can be determined by diluting the antibiotic solution to the noninhibitory level of the chro- mogenic reaction; amounts[r] ...
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Airborne Endotoxin from Indoor and Outdoor Environments: Effects of Sample Dilution on the Kinetic Limulus Amebocyte Lysate (LAL) Assay.
... kinetic Limulus amebocyte lysate (LAL) ...open-face endotoxin samples, while button samples displayed little ...airborne endotoxin concentrations were determined. The highest ...
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Chromogenic Limulus amoebocyte lysate assay for rapid detection of gram negative bacteriuria
... For assay, urine dilutions of not less than 1:10 were neces- sary for the following reasons: (i) the natural color of most urine specimens would either mask reading of a positive CLAL as[r] ...
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Evaluation of the endotoxin binding efficiency of clay minerals using the Limulus Amebocyte lysate test: an in vitro study
... kinetic chromogenic Limulus Amebocyte lysate test was adapted to measure endotoxin ...(10 endotoxin units/mL (EU/mL)) was ...increasing endotoxin concentra- tions (2,450 – ...
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Differential blocking of coagulation activating pathways of Limulus amebocyte lysate
... In this study, we have attempted to develop specific LAL tests for endotoxin and P-glucan by differentially blocking the two activation pathways of LAL, using our newly developed Limulus[r] ...
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<p>Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples</p>
... 4 Endotoxin activity in dialysate can be measured using limulus amebocyte lysate (LAL), which is puri fi ed from the blood of amebocytes of horseshoe crabs, as a ...5 Endotoxin activates ...
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New, sensitive rocket immunoelectrophoretic assay for measurement of the reaction between endotoxin and Limulus amoebocyte lysate
... Antigens, 5 IlI of the diluted LAL plus CSF mixtures was applied to the wells; antibody, polyspecific antibody against LAL in the whole gel (7.5 jll/cm2); control, from a patient suspect[r] ...
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Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate based assays and a human monocyte activity assay
... Kinetic chromogenic LAL assay was more sensitive to ...LAL assay than the PyroGene ® rFC-based ...rFC-based assay compared to the LAL-based ...
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Lyophilized Amebocyte Lysate Products and Service
... Turbidimetric Endotoxin Assay Kinetic Turbidimetric Endotoxin Assay is a simple measurement for endotoxin quantifi- ...of Amebocyte Lysate becomes turbid, the increase ...
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Rapid diagnosis of gram negative bacterial meningitis by the Limulus endotoxin assay
... Rapid detection of Gram negative bacterial meningitis by the Limulus lysate test. Latex agglutination test for the diagnosis of Hae-[r] ...
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Newly Developed Endotoxin Measurement Method (the Endotoxin Activity Assay) May Reflect the Severity of Sepsis
... The chromogenic limulus amebocyte lysate assay has been most widely used to detect ET contamination of drugs and fluids; however, its utility in biological samples has been limited, ...
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Prognostic value of endotoxin activity assay in patients with severe sepsis after cardiac surgery
... EAA: Endotoxin activity assay; ICU: Intensive care unit; IL- 6: Interleukin-6; LAL: Limulus Amebocyte Lysate; LPS: Lipopolysacharide; LVEF: Left ventricle ejection fraction; PCT: ...
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Sensitivity of Limulus amebocyte lysate (LAL) to LAL reactive glucans
... The sensitivity of Limulus amebocyte lysate (LAL) to LAL-reactive glucans (LRGs) and lipid A was tested by using commercially available and experimentally formulated LAL reagents.. The g[r] ...
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Principles of a quantitative assay for bacterial endotoxins in blood that uses Limulus lysate and a chromogenic substrate
... present in normal human plasma (heparinized) to und that heating serum is a satisfactory which a trace of '25I-labeled bovine serum albumin had for inactivation of endogenous amidases.. [r] ...
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Rapid detection of Gram negative bacteriuria by Limulus amoebocyte lysate assay
... The majority of the patient urine specimens with quantitative counts of >105 gram-negative bacteria per ml yielded positive LAL tests after. only 10 min of incubation, with 31 out of [r] ...
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Statistical determination of endotoxin content in influenza virus vaccine by the limulus amoebocyte lysate test
... To determine laboratory-to-laboratory variability of the limulus amoebocyte lysate (LAL) test for evaluating endotoxin content in influenza virus vaccine, a collaborative study was desig[r] ...
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Comparison of plasma extraction techniques in preparation of samples for endotoxin testing by the Limulus amoebocyte lysate test
... Comparison of Limulus amoebocyte lysate test results from healthy human plasma samples extracted by these two methods indicated that lysate type and not extraction procedure was associat[r] ...
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Sensitivity, specificity, and predictive values of the Limulus lysate assay for detection of exclusion of gonococcal cervicitis
... After the removal of 0.1 ml from the cervical samples for assay in water, 0.05 ml of the dispersing agent was added to the tube containing the swab.. The tubes were Vortex mixed, and 0.5[r] ...
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