enzyme linked immunosorbent spot assay
Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme linked immunosorbent spot assay for detection of mycobacteria
... ELISA spot assay. The capture spot ELISA was adapted from the assay of Spira and Scharff (15) for the purpose of capturing and visualizing a single ...
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Development of an Enzyme-Linked Immunosorbent Spot Assay To Measure Serum-Neutralizing Antibodies against Coxsackievirus B3
... In this study, we developed an ELISPOT assay to measure serum- neutralizing antibodies against CVB3. Because the traditional neutralization tests are time-consuming and labor-intensive, sev- eral different ...
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Comparison of Two Gamma Interferon Release Assays and Tuberculin Skin Testing for Tuberculosis Screening in a Cohort of Patients with Rheumatic Diseases Starting Anti-Tumor Necrosis Factor Therapy
... an enzyme-linked immunospot release assay (T- ...an enzyme-linked immunosorbent assay (QuantiFERON-TB Gold In Tube [QFT-GIT]), to that of tuberculin skin testing (TST) for ...
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Wave_IV_hsCRP_EBV_Documentation.pdf
... the enzyme-linked immunosorbent assay are provided below, but in brief, punches from dried blood spot calibrator, quality control sample, or respondent sample cards were eluted in a ...
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Rubella sero-prevalence among children in Kilimanjaro region: a community based study prior to the introduction of rubella vaccine in Tanzania
... Background: Childhood rubella infection is a mild, self-limiting illness. Rubella infection among pregnant women however, is a major public health concern. Depending on gestation age, it may result in fetal death, ...
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Enzyme linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus
... CHIKV assay, suggesting it may detect low levels of the antibodies ...immunosorbend assay, suggesting it is a CHIKV-specific ...the assay is compared to a CHIKV-specific neutralization ...
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Surface immunofluorescence assay for diagnosis of Lyme disease
... A surface immunofluorescence assay SIFA was analyzed and compared with a conventional indirect immunofluorescence assay IFA and whole-cell enzyme-linked immunosorbent assay ELISA for det[r] ...
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Comparison of enzyme linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens
... the assay antigen, which in turn is allowed to react with the test serum supposedly containing the primary antibody followed by an enzyme-labeled secondary ...the assay antigen and the IgM from test ...
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Comparison of a Classical Phagocytosis Assay and a Flow Cytometry Assay for Assessment of the Phagocytic Capacity of Sera from Adults Vaccinated with a Pneumococcal Conjugate Vaccine
... phagocytosis assay for pneumococci would be a great asset for the evaluation of the potential efficacy of (experimental) pneumococcal ...an assay could replace the laborious phagocytosis assay of ...
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Assay of antibody to group A streptococcal carbohydrate by enzyme linked immunosorbent assay
... This test has been used to measure antibody to the group A carbohydrate ACHO in the serum of patients with group A streptococcal infections and their complications, e.g., nephritis or rh[r] ...
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Measurement of rotavirus antibody by an enzyme linked immunosorbent assay blocking assay
... Since virus obtained from human stool can be used for the blocking assay, there is no need for a gnotobiotic animal source of the human rotavirus.. Results of the ELISA blocking assay co[r] ...
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Comparison of enzyme linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions
... The purpose of this study was to simultaneously compare enzyme-linked immunosorbent assay ELISA, immunofluorescence assay IFA, and the standard virus isolation technique VIT for the dete[r] ...
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Evaluation of a Novel Aspergillus Antigen Enzyme Linked Immunosorbent Assay
... GP assay, a procedure that is recommended by the manufacturer of the GM assay in the case of initial positive results, yielded negative results except for one FIG 6 Results of antigen testing in all sera of ...
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Enzyme linked immunosorbent assay for Clostridium difficile toxin A
... difficile strains by the ELISA for the presence of toxin A, and our results demonstrated that the concentration of toxin A by ELISA closely correlated with the cytotoxic titers of toxins[r] ...
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Enzyme linked immunosorbent assay for streptococcal M protein antibodies
... Protein fraction a from type M-12, group A streptococcal cells elicits bactericidal antibodies in rabbits and has opsonic inhibitory activity 21.. Therefore, fraction a was used in the E[r] ...
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Enzyme Linked Immunosorbent Assay for Immunological Diagnosis of Human Tularemia
... tularensis LPS and ratio of relative IgGI IgM titers of sera obtained from healthy controls and from patients with tularemia in the acute phase of disease and 2.5 years after infection N[r] ...
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Enzyme linked immunosorbent assay for detection of Mycoplasma pneumoniae antibodies
... Determination of the optimal antigen concentrations was done using varied dilutions of antigen 1.5 to 48 lig of proteins per well, a positive reference serum diluted 1:16, and conjugated[r] ...
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Detection of Salmonella infections by polyvalent enzyme linked immunosorbent assay
... values MPEV for each Salmonella assay were calculated by adding 2 standard deviations to the mean of tested reference negative sera.. A positive ELISA value was defined as greater than o[r] ...
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Enzyme linked immunosorbent assay for detection of Pseudomonas aeruginosa Lipopolysaccharides
... When LPS in purified preparations or in culture filtrates was examined with both homologous and heterologous antibody systems, the same specificity pattern was demonstrated, suggesting t[r] ...
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Optimization of a Human Papillomavirus-Specific Enzyme-Linked Immunosorbent Assay
... the assay and determination of a COV inde- pendent of those determined in prior ...ensure assay reproducibility over time and to determine an optimal COV for ELISA screening of serologic responses to ...of ...
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