ESAT-6/CFP10
A peptide fragment from the human COX3 protein disrupts association of Mycobacterium tuberculosisvirulence proteins ESAT 6 and CFP10, inhibits mycobacterial growth and mounts protective immune response
... tate ESAT-6 and counter its debilitating functions on host ...binds ESAT-6, disrupts ESAT-6:CFP10 association, and inhibits the growth of ...with ESAT-6, ...
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Diagnosis of Tuberculosis Based on the Two Specific Antigens ESAT-6 and CFP10
... of ESAT-6 and CFP10 is highly specific for ...in ESAT-6-positive and -negative contacts, and this may give an indication of the po- tential of such antigens for the diagnosis of ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Protein expression of Recombinant ESAT-6 andCFP-10. The pET32a(+)-ESAT-6 and pET32a(+)- CFP10 plasmids were transformed into E. coli BL21 DE3 (Novagen, Germany) expression host with ...
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Rv1985c, a promising novel antigen for diagnosis of tuberculosis infection from BCG vaccinated controls
... and ESAT-6, it is very possible to improve the diagnostic performance of ESAT-6 and/or CFP-10 by addition of ...for ESAT-6 Table 2 Characterization of IFN-g ELISPOT in response ...
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A T cell based assay using specific Mycobacterium tuberculosis antigens, ESAT-6 and CFP 10, for the diagnosis of Tuberculous Meningitis.
... target 6-kD protein (ESAT-6) was identified from culture filtrate because of its strong recognition in animals infected with M tuberculosis ...tuberculosis ESAT-6 gene was cloned and ...
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Recognition of Stage-Specific Mycobacterial Antigens Differentiates between Acute and Latent Infections with Mycobacterium tuberculosis
... of ESAT-6-responsive, healthy individuals without recent TB contact as latently ...by ESAT-6 responsiveness and estimate the risk that it is progressive or latent by determining the ratio of ...
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Assessment of Mycobacterium tuberculosis OmpATb as a Novel Antigen for the Diagnosis of Bovine Tuberculosis
... an ESAT-6–CFP-10 peptide cocktail (E/C) as described previously (13), and the cocktail was used at a concentration of 5 ...at 6 months of age as described previously ...
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Field Evaluation of a Novel Differential Diagnostic Reagent for Detection of Mycobacterium bovis in Cattle
... In the search for improved tools with which to control bovine tuberculosis, the development of enhanced immunodiagnostic reagents is a high priority. Such reagents are required to improve the performance of tuberculin- ...
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The multistage vaccine H56 boosts the effects of BCG to protect cynomolgus macaques against active tuberculosis and reactivation of latent Mycobacterium tuberculosis infection
... Overview of experimental design. Experiment 1 included 3 arms (n = 6/group): (a) control; (b) BCG only; (c) BCG followed by H56 at 14 and 17 weeks. All groups were infected with high-dose M. tuberculosis (500 CFU) ...
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Rapid immunodiagnostic assays for Mycobacterium Tuberculosis infection
... In an effort to develop more accurate diagnostic tools, recent studies have led to the identification of the ge- nomic segment BCG-region 1 (RD1), present in MTB but absent from all strains as well as almost all ...
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Selected Pool of Peptides from ESAT 6 and CFP 10 Proteins for Detection of Mycobacterium tuberculosis Infection
... The “gold standard” for the diagnosis for TB is a combina- tion of clinical and radiologic examination and microbiological tests, both microscopic and cultural (the last one can take up to 8 weeks, and in 10 to 20% of ...
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Identification of Cytokines in Whole Blood for Differential Diagnosis of Tuberculosis versus Pneumonia
... tration of the right upper lobe apical and posterior segments or the left lobe apical-posterior segment, or a definitive diagnosis of TB established by isolation of tubercle bacilli in culture or identification of ...
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Quantitative investigation of factors relevant to the T cell spot test for tuberculosis infection in active tuberculosis
... tube; 6–9 ml of diluted whole blood was then slowly added to the solution, and the tube was centri- fuged at 1000 x g for 22 ...10 6 cells/ml. (6) Fifty microliters of negative control (ALM- V), ...
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Red Spectral Shift in Sensitive Colorimetric Detection of Tuberculosis by ESAT 6 Antigen Antibody Complex: a New Strategy with Gold Nanoparticle
... of ESAT-6, only a small amount of antibodies are ...with 6 kDa molecular weight ESAT-6, which has more molecules even at a lower ...
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Safety of Recombinant Fusion Protein ESAT6-CFP10 as a Skin Test Reagent for Tuberculosis Diagnosis: an Open-Label, Randomized, Single-Center Phase I Clinical Trial
... and CFP10 that is analogous to the IGRAs is speculated to be more specific than the conventional ...and CFP10 (weight ratio of 1:1) as the skin test reagent for the diagnosis of ...
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MTB-specific lymphocyte responses are impaired in tuberculosis patients with pulmonary cavities
... Methods: We utilized culture filtrate protein 10 (CFP-10) and early secretory antigenic target 6 (ESAT-6) as immuno- genic MTB antigens following overnight stimulation of peripheral blood mononuclear ...
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Original Article Analysis of differential proteins of pleural effusion cells from Mycobacterium tuberculosis infected patients
... A: Percentage map of Atg85B, PRv1269c, and ESAT-6 detected in pleural effusion cells of fifty tuberculosis patients by Western blotting; B: Detection rate of tuberculosis patients by thr[r] ...
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Construction of Eukaryotic Expression Vectors Encoding CFP-10 and ESAT-6 Genes and Their Potential in Lymphocyte Proliferation
... and 6-kDa early-secreted target antigen (ESAT-6) are two low molecular weight secretory proteins encoded by the Rv3874 and Rv3875 genes, ...The ESAT-6/CFP-10 complex’s secretion from ...
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The ESAT 6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram positive bacteria
... Schematic representation of the genomic organization of the genes present in the five ESAT-6 gene cluster regions of Mycobacterium tuberculosis H37Rv as well as the regions in C. diphtheriae and S. ...
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Recognition of ESAT-6 Sequences by Antibodies in Sera of Tuberculous Nonhuman Primates
... to ESAT-6 protein and ESAT-6-derived peptides in one rhesus macaque (A) and one cynomolgus macaque ...against ESAT-6 protein and eight ESAT-6-derived, overlapping ...
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