Escherichia coli heat-labile enterotoxin
Functional Pentameric Formation via Coexpression of the Escherichia coli Heat-Labile Enterotoxin B Subunit and Its Fusion Protein Subunit with a Neutralizing Epitope of ApxIIA Exotoxin Improves the Mucosal Immunogenicity and Protection against Challenge by Actinobacillus pleuropneumoniae
... the Escherichia coli heat-labile enterotoxin B subunit (LTB) and a fusion protein of an ApxIIA toxin epitope produced by Actinobacillus pleuropneumoniae coupled to LTB, respectively, ...
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Human Papillomavirus Virus-Like Particles Are Efficient Oral Immunogens when Coadministered with Escherichia coli Heat-Labile Enterotoxin Mutant R192G or CpG DNA
... with Escherichia coli heat-labile enterotoxin (LT) mutant R192G (LT R192G) or CpG DNA can significantly improve anti-VLP humoral responses in peripheral blood and in genital mucosal ...
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GM1 erythroimmunoassay for detection and titration of Escherichia coli heat labile enterotoxin
... Method for the detection and dosage of the Escherichia coli heat-labile enterotoxin by an enzyme-linked immunosorbent assay using a new simple kit test.. Production of "Escherichia coli"[r] ...
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Improved GM1 enzyme linked immunosorbent assay for detection of Escherichia coli heat labile enterotoxin
... Previously described GM1 ganglioside enzyme-linked immunosorbent assays GM1-ELISA for the detection of Escherichia coli heat-labile enterotoxin LT showed sensitivity equal to the Y-1 adr[r] ...
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Expression of Escherichia coli heat labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L ) fruit
... (Escherichia coli heat-labile enterotoxin B subunit) via Agrobacterium-mediated transformation of tomato (Solanum lycopersicum ...
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Enzyme linked immunosorbent assay for detection of Escherichia coli heat labile enterotoxin
... The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E.. coli enterotoxin but requires neither radioactive reagents n[r] ...
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Specificity of Escherichia coli Heat-Labile Enterotoxin Investigated by Single-Site Mutagenesis and Crystallography
... enterotoxigenic Escherichia coli is one of the leading causes of mortality in children under five years of age and is a great burden on developing ...the heat-labile enterotoxin (LT), a ...
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Solid Phase Microtiter Radioimmunoassay Blocking Test for Detection of Antibodies to Escherichia coli Heat Labile Enterotoxin
... Again the actual antibody titer of these sera was considerably higher in the RIA blocking test than the adrenal cell neutralization assay; however, the relative correlation of the two te[r] ...
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Comparison of methods to detect Escherichia coli heat labile enterotoxin in stool and cell free culture supernatants
... In addition, the ELISA of stool supernatants detected more samples known to be positive by YAC assay of pooled culture supernatants than did the ELISA of culture supernatants, possibly o[r] ...
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Evaluation of a ganglioside immunosorbent assay for detection of Escherichia coli heat labile enterotoxin
... In this study an A4001° value of 0.4 above the background level in the GM1-ELISA and a 1+ reaction in the adrenal cell test were both considered as positive results when undiluted cultur[r] ...
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Gangliosides sensitize unresponsive fibroblasts to Escherichia coli heat labile enterotoxin
... E. coli heat-labile ...E. coli heat-labile enterotoxin based on the ability of the free ganglioside to inhibit the effects of ...
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Rapid GM1 enzyme linked immunosorbent assay with visual reading for identification of Escherichia coli heat labile enterotoxin
... All the cultures of the strains previously shown to produce LT had a significant enterotoxin titer as determined with the GM1-AP-ELISA and also.. the cultures to a final concentration of[r] ...
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Necrotizing Enterocolitis with Escherichia coli Heat-Labile Enterotoxin
... Of affected babies, 15 had toxigenic Escherichia coli or heat-labile E coli toxin in feces de-.. tected by enzyme-linked immunosorbent assay.[r] ...
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Activation of Adenylate Cyclase by Heat Labile Escherichia Coli Enterotoxin: EVIDENCE FOR ADP RIBOSYLTRANSFERASE ACTIVITY SIMILAR TO THAT OF CHOLERAGEN
... weight Escherichia coli heat-labile enterotoxin (LT) catalyzed the hydrolysis of NAD to ADP-ribose and ...the enterotoxin had mobilities on ...
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Comparison of two GM1 erythrocyte assays to detect heat labile Escherichia coli enterotoxin in stool specimens
... Two erythrocyte immunoassay techniques to detect the presence of Escherichia coli heat-labile enterotoxin LTh in stool supernatants and cell-free culture supernatants were compared.. In [r] ...
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Intranasal Immunization of Mice with CpG DNA Induces Strong Systemic and Mucosal Responses That Are Influenced by Other Mucosal Adjuvants and Antigen Distribution
... One of the major impediments to the de- velopment of mucosal vaccines has been the lack of effective, yet safe, mucosal adjuvants. Cholera toxin (CT) and the structurally related Escherichia coli ...
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Automatic and manual latex agglutination tests for measurement of cholera toxin and heat labile enterotoxin of Escherichia coli
... M glycine-NaOH buffer This paper deals with automatic and manual Dow latex suspension inM0.05 8.2 containing 0.05 sodium chloride was mixed latex agglutination tests for cholera toxin CT[r] ...
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Trivalent heat labile and heat stable enterotoxin probe conjugated with horseradish peroxidase for detection of enterotoxigenic Escherichia coli by hybridization
... The HRP-conjugated trivalent probe was applied to the detection of enterotoxigenic Escherichia coli ETEC by colony and stool hybridizations.. The binding of the probe to its targets was [r] ...
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Heat Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into Neurons
... plants and comprise a monomeric enzymatic A subunit and a binding (B) subunit pentamer. The A subunit is a single polypep- tide composed of two domains, A1 and A2, which are linked to- gether via a disulfide bond. The A1 ...
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Ganglioside GM1 enzyme linked immunospot assay for simple identification of heat labile enterotoxin producing Escherichia coli
... coli strains, results obtained by this procedure GM1ELISPOT correlated well with those of the GM1 enzyme-linked immunosorbent assay GM1-ELISA; in no instance were any false-positive reac[r] ...
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