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ethidium bromide acridine orange

Evaluation of In Vitro Cytotoxic Effects of Three Medicinal Plants on Peripheral Blood Mononuclear Cells (PBMC)

Evaluation of In Vitro Cytotoxic Effects of Three Medicinal Plants on Peripheral Blood Mononuclear Cells (PBMC)

... using Acridine Orange and Ethidium Bromide staining and a significant increase in the apoptotic features were noticed in cells treated with the plant ...

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Antioxidant and anti-proliferative activity of different solvent extracts of Casuarina equisetifolia needles

Antioxidant and anti-proliferative activity of different solvent extracts of Casuarina equisetifolia needles

... (22). Acridine orange/ethidium bromide staining of MCF-7 cells treated with GI 50 concentrations of non polar solvent extracts for 48 hours showed significant apoptosis as cells stained as ...

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“Apoptogenic Activity of Secretion Extract of Bellamya
Bengalensis f. Annandalei via Mitochondrial Mediated Caspase Cascade on
Human Leukemic Cell Lines” by Shila Elizabeth Besra,Moumita Ray,
Sayantan Dey, Subhadeep Roy, Nilanjana Deb, India.

“Apoptogenic Activity of Secretion Extract of Bellamya Bengalensis f. Annandalei via Mitochondrial Mediated Caspase Cascade on Human Leukemic Cell Lines” by Shila Elizabeth Besra,Moumita Ray, Sayantan Dey, Subhadeep Roy, Nilanjana Deb, India.

... several morphological changes such as cell shrinkage, membrane blebbing, chromatin condensation, nuclear fragmentation and formation of apoptotic bodies. Fluorescence microscopic images clearly showed nuclear ...

7

Upregulation of nucleostemin in colorectal cancer and its effects on cell malignancy

Upregulation of nucleostemin in colorectal cancer and its effects on cell malignancy

... by acridine orange/ethidium bromide double staining of the cells (Figure ...stained orange and showed condensed and fragmented ...

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The effect of two novel amino acid coated magnetic nanoparticles on survival in vascular endothelial cells, bone marrow stromal cells, and macrophages

The effect of two novel amino acid coated magnetic nanoparticles on survival in vascular endothelial cells, bone marrow stromal cells, and macrophages

... Abbreviations AO: acridine orange; BMSCs: bone marrow stromal cells; EB: ethidium bromide; FBS: fetal bovine serum; FGF-2: basic fibroblast growth factor; Gly: glycine; HUVECs: human umb[r] ...

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Study of Linear Optical Properties of Acridine-orange Laser Dye

Study of Linear Optical Properties of Acridine-orange Laser Dye

... The results showed that the linear absorption coefficient and linear- refractive index increase with solution concentration increasing and (life-time of fluorescence inc[r] ...

5

Enhancement of Commercial Production of Polymeric PHB Material from Bacterial Strains through Mutagenic Strategies

Enhancement of Commercial Production of Polymeric PHB Material from Bacterial Strains through Mutagenic Strategies

... Poly- βββββ -Hydroxybutyrate (PHB) is proving itself as an advance polymeric material as well as an alternative for plastic material. The only hinge stopping large scale utilization of PHB at industrial scale and in ...

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Screening of heterozygous DNA markers in shiitake (Lentinula edodes) using de-dikaryotization via preparation of protoplasts and isolation of four meiotic monokaryons from one basidium

Screening of heterozygous DNA markers in shiitake (Lentinula edodes) using de-dikaryotization via preparation of protoplasts and isolation of four meiotic monokaryons from one basidium

... Polymerase chain reaction (PCR) products were separated on 1.0% agarose gel with ethidium bromide.. Distortions of the segregation ratio that may be caused by the differe[r] ...

6

Fluorouracil Selectively Enriches Stem-like Leukemic Cells in a Leukemic Cell Line

Fluorouracil Selectively Enriches Stem-like Leukemic Cells in a Leukemic Cell Line

... In the present study, the 5-FU resistant cells showed orange fluorescence in acridine orange staining test, which indicated the low-level of RNA transcription and quiescent consis- te[r] ...

9

SYBR safe efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption.

SYBR safe efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption.

... stain, in A. fumigatus gene disruption protocol. The chosen gene was cipC, which has already been disrupted successfully in our laboratory. A deletion cassette was constructed in Saccharomyces cerevisiae and used in A. ...

5

Detection of borreliacidal antibody by using acridine orange and flow cytometry.

Detection of borreliacidal antibody by using acridine orange and flow cytometry.

... The flow cytometric parameters of side scatter and detection of acridine orange fluorescence were used to determine events per minute (number of labeled spirochetes), percent shift in fl[r] ...

7

Evaluation of acridine orange stain for detection of microorganisms in blood cultures

Evaluation of acridine orange stain for detection of microorganisms in blood cultures

... Even when radiometric methods are employed A total of 10 9.2% cultures were detected by to detect microorganisms in blood culture, only the AO method, and 14 12.8% were de- "blind" subcu[r] ...

5

Improvement of malaria diagnostic system based on acridine orange staining

Improvement of malaria diagnostic system based on acridine orange staining

... Conclusions: Consistent AO staining of thin blood films and rapid evaluation of malaria parasitaemia with the revised protocol produced superior results relative to the Giemsa method.. [r] ...

6

A method for visualizing surface exposed and internal PfEMP1 adhesion antigens in Plasmodium falciparum infected erythrocytes

A method for visualizing surface exposed and internal PfEMP1 adhesion antigens in Plasmodium falciparum infected erythrocytes

... Figure 4 illustrates the resolution of surface detectable PfEMP1 on the erythrocyte membrane obtained using this methodology to assess membrane loading of PfEMP1 dur- ing asexual parasite development. The image shows the ...

9

A poxvirus-encoded uracil DNA glycosylase is essential for virus viability.

A poxvirus-encoded uracil DNA glycosylase is essential for virus viability.

... Ethidium bromide fluorescence assay of the refolded SFV uracil DNA glycosylase using uracil-containing plasmid DNA as a substrate.. Following treatment with the enzyme, the fluorescence [r] ...

10

Genetic basis of resistance to quaternary ammonium compounds – the qac genes and their role: a review

Genetic basis of resistance to quaternary ammonium compounds – the qac genes and their role: a review

... Although the qac genes are named after one of their main substrates (QACs), the spectrum of their activity is much wider. qac-mediated resistance tar- gets more than 30 lipophilic cationic compounds belonging to at least ...

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Focal Injection of Ethidium Bromide as a Simple Model to Study Cognitive Deficit and Its Improvement

Focal Injection of Ethidium Bromide as a Simple Model to Study Cognitive Deficit and Its Improvement

... Demyelination plaques within the hippocampus have been reported in clinical studies using MRI technique (Geurts et al., 2007; Rao et al., 1991; Sailer et al., 2003). The chemical toxin, EB, is commonly used to induce lo- ...

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Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

... Ethidium Bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since ...by ethidium bromide as ...

13

The Effect of Vitamin E and Selenium on Sperm Chromatin Quality in Couples with Recurrent Miscarriage

The Effect of Vitamin E and Selenium on Sperm Chromatin Quality in Couples with Recurrent Miscarriage

... Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion.[r] ...

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Polyhydroxybutyrate (PHB) Production and Mutagenesis of Halophile isolates from the East African Rift Salt Lake

Polyhydroxybutyrate (PHB) Production and Mutagenesis of Halophile isolates from the East African Rift Salt Lake

... implemented in which high carbon source in the form of dextrose was given to the bacteria at regular intervals, without increasing the nitrogen source. Further studies were carried out on random mutagenesis approach ...

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