eukaryotic protein synthesis initiation factor-4E
Differential regulation of protein synthesis in skeletal muscle and liver of neonatal pigs by leucine through an mTORC1-dependent pathway
... of protein synthesis, and ribosomal protein S6 kinase 1 (S6K1) and eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1) phosphorylation in ...
10
Eukaryotic Initiation Factor 4GII (eIF4GII), but Not eIF4GI, Cleavage Correlates with Inhibition of Host Cell Protein Synthesis after Human Rhinovirus Infection
... containing an extra sequence of 46 aa from the pBlueBacHis2 C baculovirus vector (Invitrogen) (16, 20). The ECL signal of the HeLa S10 corresponded to that elicited by 34 ng of eIF4GI. We calculated that eIF4GI ...
6
Eukaryotic initiation factor 4E 3 is essential for meiotic chromosome segregation, cytokinesis and male fertility in Drosophila
... in protein synthesis during ...chaperone protein that is required for activation of CDC2A kinase, which regulates the prophase to metaphase ...chaperone protein, CG12020, is among the proteins ...
10
Complex Formation between Potyvirus VPg and Translation Eukaryotic Initiation Factor 4E Correlates with Virus Infectivity
... on protein synthesis has not been intensively investigated ...translation initiation minimize the rate of entry of mRNA into decay pathways ...
8
Identification in the Ancient Protist Giardia lamblia of Two Eukaryotic Translation Initiation Factor 4E Homologues with Distinctive Functions
... Only eIF4E2 plays an essential function in protein synthesis in Giardia. To elucidate whether eIF4E1, eIF4E2, or both perform an essential function in Giardia, an antisense-ri- bozyme strategy with a viral ...
12
Original Article Expression of eukaryotic initiation factor 4E and 4E binding protein 1 in colorectal carcinogenesis
... The eIF4E expressed is subject to phosphoryla- tion as a downstream target of the MAPK sig- naling pathway [3]. The results of the immuno- histochemical staining for the detection of p-eIF4E failed to reveal the presence ...
10
The Lymphocytic Choriomeningitis Virus RING Protein Z Associates with Eukaryotic Initiation Factor 4E and Selectively Represses Translation in a RING-Dependent Manner
... affected protein synthesis. We show that the Z protein can repress specific protein production at the posttranscriptional and post- RNA transport levels in cell ...decreased protein ...
8
Regulation of protein degradation pathways by amino acids and insulin in skeletal muscle of neonatal pigs
... muscle protein synthesis and how this changes with ...in protein degradation ...RING-finger protein-1 (MuRF1) and autophagy-lysosome systems, ...microtubule-associated protein light ...
11
Protein Synthesis and Endoplasmic Reticulum Stress Can Be Modulated by the Hepatitis C Virus Envelope Protein E2 through the Eukaryotic Initiation Factor 2α Kinase PERK
... response and mediates the folding of newly synthesized pro- teins in the ER lumen (for a review, see reference 13). PERK is held at the ER in an inactive, monomeric form by BiP and possibly by GRP94 (1, 15) (Fig. 6A). ...
8
Multiple isoelectric forms of poliovirus RNA-dependent RNA polymerase: evidence for phosphorylation.
... The host cell protein required for in vitro replication of poliovirus is a protein kinase that phosphorylates eukaryotic initiation factor 2.. ATP is required for initiation of polioviru[r] ...
6
Therapeutic suppression of translation initiation modulates chemosensitivity in a mouse lymphoma model
... Results are expressed as mean ± SEM of 2 experiments. (B) CBFs primarily impact protein synthesis in vivo. HeLa cells were incubated with 5 μM FA, 0.4 μM silvestrol, or vehicle (MeOH) for 1 hour. The rate ...
11
Visualization of the Interaction between the Precursors of VPg, the Viral Protein Linked to the Genome of Turnip Mosaic Virus, and the Translation Eukaryotic Initiation Factor iso 4E In Planta
... Cellular localizations of GFP fusions of eIF(iso)4E, VPg- Pro, and 6K-VPg-Pro. To further refine their cellular localiza- tions, eIF(iso)4E, VPg-Pro, and 6K-VPg-Pro were fused to the green fluorescent ...
8
mRNA Translation Regulation by the Gly-Ala Repeat of Epstein-Barr Virus Nuclear Antigen 1
... The observation that introduction of a serine in the N- terminal part of the GAr peptide has a more pronounced effect on GAr function than inserting serines in the C terminus sug- gests that the nascent GAr peptide is ...
10
Download Download PDF
... studies that reported a role of the PERK/eIF2α/CHOP signal- ing pathway in neuronal apoptosis. Specifically, in an in vitro model of ER stress in cortical neurons, ATF4 induced CHOP expression, which enhanced the ...
6
Avian Reovirus Nonstructural Protein p17-Induced G2/M Cell Cycle Arrest and Host Cellular Protein Translation Shutoff Involve Activation of p53-Dependent Pathways
... viral protein expression and prog- eny virus production were greater in G 2 /M-phase-arrested ...p17 protein play a direct role, partially explaining the p17 constant movement in and out of the nucleus (36, ...
13
Human Cytomegalovirus Infection Induces Rapamycin-Insensitive Phosphorylation of Downstream Effectors of mTOR Kinase
... of 4E-BP seen at 12 and 24 h after infection is due to a declining effect of the drug or incomplete inhibition of ...stimulate 4E-BP ...of 4E-BP was ...of 4E-BP were present in the serum ...
10
Ribosomal RACK1 promotes chemoresistance and growth in human hepatocellular carcinoma
... translation initiation is coupled with cell cycle progression and cell growth, whereas excessive ribosome biogenesis and translation initiation often lead to tumor transformation and ...of eukaryotic ...
14
Low-arginine and low-protein diets induce hepatic lipid accumulation through different mechanisms in growing rats
... dietary protein [8, 9] and amino acids [10] on energy homeostasis are less well understood despite their importance in the pathogenesis of ...dietary protein [11] and amino acid intake [12]. Protein ...
13
Hinrichs, Arne (2018): Generation and characterization of a pig model for Laron syndrome. Dissertation, LMU München: Tierärztliche Fakultät
... Laron Syndrome (LS) is a very rare autosomal recessive hereditary disorder – just a few hundred people are affected worldwide (Rosenfeld, Rosenbloom et al. 1994). LS is caused by mutations of the growth hormone receptor ...
114
PC-1/PrLZ confers resistance to rapamycin in prostate cancer cells through increased 4E-BP1 stability
... Tissue microarrays of 40 prostate tumors were purchased from Shanxi Chaoying Biotechnology Co., Ltd. and the clinicopathologic features of all 40 patients were summarized in Supplementary Table 1. The microarrays were ...
14