Extractable Nuclear Antigens (ENA)
Practical Evaluation of Methods for Detection and Specificity of Autoantibodies to Extractable Nuclear Antigens
... against extractable nuclear antigens (ENA) play a critical role in the diagnosis and management of autoimmune ...specificity ENA autoantibodies. To address the relationship of ...
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Detection of Anti Extractable Nuclear Antigens in Patients with Systemic Rheumatic Disease via Fluorescence Enzyme Immunoassay and Its Clinical Utility
... Purpose: Testing for autoantibodies to extractable nuclear antigens (ENAs) plays an important role in the diagnosis and manage- ment of systemic rheumatic disease. Currently, no gold standard tests ...
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Autoantibodies to Extractable Nuclear Antigens: Making Detection and Interpretation More Meaningful
... Over the last decade, new methods of performing immunol- ogy tests have been introduced into laboratory practice, prin- cipally to facilitate the processing of large numbers of samples. This rapid change has compounded ...
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Antibodies against specific extractable nuclear antigens (ENAs) as diagnostic and prognostic tools and inducers of a profibrotic phenotype in cultured human skin fibroblasts: are they functional?
... sulfoxide; ENA: Extractable nuclear antigen; EULAR: European league against rheumatism; FBS: Fetal bovine serum; FITC: Fluorescein isothiocyanate; GAPDH: Glyceraldehyde 3- phosphate dehydrogenase; ...
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The MRZ reaction helps to distinguish rheumatologic disorders with central nervous involvement from multiple sclerosis
... Anti- nuclear antibody (ANA) staining pattern was assessed using indirect immunofluorescence (IIF) on 2100-Ro HEp-2000® cells (Fluorescent ANA-RoTest System, Immuno Concepts ...against extractable ...
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Evaluation of Multiplexed Fluorescent Microsphere Immunoassay for Detection of Autoantibodies to Nuclear Antigens
... to extractable nuclear antigens (ENA) are found in a variety of collagen vascular ...useful ENA was measured in 249 serum samples, including samples from 56 patients previously ...
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Decreased frequency and activated phenotype of blood CD27 IgD IgM B lymphocytes is a permanent abnormality in systemic lupus erythematosus patients
... negative; ENA: extractable nuclear antigens; FC: flow cytometry; FITC: fluorescein isothio- cyanate; GC: germinal center; IIF: indirect immunofluorescence; ...
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Review for the generalist: The antinuclear antibody test in children - When to use it and what to do with a positive titer
... substrate; ENA: extranuclear antigens; MCTD: mixed connective tissue disease; JRA: juvenile rheumatoid arthritis; [(JIA): also known as juvenile idiopathic arthritis]; JDM: juvenile dermatomyositis; dsDNA: ...
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Detection of antibodies to extractable nuclear antigens by ELISA for the diagnosis of connective tissue disorders in ANA positive patients
... different antigens and nuclear extract of Hep-2 ...the antigens or Hep-2 nuclear extract, the antibody will bind to the immobilized antigen on 1 or more of the bead ...
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Predictive biological markers of systemic lupus erythematosus flares: a systematic literature review
... against extractable nuclear antigens; Anti-RNP: Anti-ribonucleoprotein; BAFF: B-cell activating factor from the TNF family; BILAG: British Isles Lupus Assessment Group; BLyS: B-lymphocyte stimulating ...
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Ultraviolet light induces binding of antibodies to selected nuclear antigens on cultured human keratinocytes
... of extractable nuclear antigens (SSA/Ro, RNP, and Sm) following irradiation was seen on injured keratinocytes whose cell membranes were intact, but not on dead ...
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HDAC Inhibition in Lupus Models
... against nuclear antigens such as nucleosomes, DNA and histone proteins found within the body’s cells and ...targeted antigens and damaging ...
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Monteith_unc_0153D_15868.pdf
... by nuclear antigens has also been implicated in ...to nuclear antigen has proved to be difficult as studies in SLE patients and murine models of SLE have produced contradicting reports on the roles ...
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Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme labile P and solution 31P NMR spectroscopy
... readily extractable phosphorus in the rhizosphere. Water- and citrate-extractable P were assessed to monitor plant-induced changes to immediately bioavailable and readily extractable ...
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Combined Fluorescent-Antibody and Electron Microscopy Study of Marek's Disease Virus-Infected Cell Culture
... Diffuse and granular cytoplasmic antigens are seen in round cells and fusiform cells inset.. The nuclear staining is not as bright as the cytoplasmic staining.[r] ...
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Intracellular Route of Canine Parvovirus Entry
... An attempt to circumvent the endocytic and membrane fu- sion steps in the entry process was made by microinjecting CPV particles into the cytoplasm. The microinjection experi- ments showed that CPV particles injected ...
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Extractable nuclear antigen effect on the DNA anti DNA reaction and NZB/NZW mouse nephritis
... to ENA are associated with a relatively benign clinical course in patients with systemic lupus erythematosus (SLE) in which DNA anti DNA complexes are thought ...of ENA and anti-ENA on DNA anti-DNA ...
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BUTANOL EXTRACTABLE IODINE OF SERUM
... For example the SPI of a patient taking 30 drops of Lugol's solution serum inorganic iodine 225 gamma per cent was measured in two samples of serum centrifuged four times with double dis[r] ...
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A simple spectrophotometric determination of Nevirapine in pharmaceutical dosage form
... chloroform extractable complex of Nevirapine with wool fast ...the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank and obeys beer’s law in ...
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Autophagy in Innate Recognition of Pathogens and Adaptive Immunity
... intracellular antigens to CD4 T cells. Intracellular antigens are engulfed by autophagosomes, transported to MHC class II loading compartments (MIIC), and then loaded onto MHC class II molecules for ...
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