Fetal bovine serum, FBS
Activity in fetal bovine serum that stimulates erythroid colony formation in fetal mouse livers is insulinlike growth factor I
... of fetal serum was characterized. Using fetal bovine serum (FBS) as a source of the erythropoietic activity and serum-free cultures of fetal mouse livers (FMLC ...
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Human umbilical cord Wharton jelly cells promote extra-pancreatic insulin formation and repair of renal damage in STZ-induced diabetic mice
... Isolation, culture and characterization of hUCWJCs The protocols for sampling human umbilical cord tissue were approved by the Institutional Review Board of Shantou University Medical College. All participants pro- vided ...
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Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice
... C57Bl/6J mice (n=46, 20-23g, Janvier Labs, FR) on standard chow diet (R36 Lactamin, SE) were used for in vitro experimentations, animals were housed in groups. Primary cultures of myenteric neurons were prepared from the ...
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Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis
... PTX with more than 98% purity was purchased from Yew Biotechnology Co. Ltd. (Jiangsu, China). Taxol (marked product of PTX) was purchased from Bristol-Myers Squibb (China) Investment Co. Ltd. (Shanghai, China). β-CN (No. ...
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The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
... 10% fetal bovine serum (FBS; Gibco, Invitrogen), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco, Invitrogen) in a humidified atmos- phere of 5% CO2 at 37 ...
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Upconversion-Magnetic Carbon Sphere for Near Infrared Light-Triggered Bioimaging and Photothermal Therapy
... To demonstrate the surface status of the FeCUPs in different mediums for better therapeutic effect, the zeta-potential (ζ) and corresponding dynamic light scattering (DLS, d) were measured (Figure S5). It was suggested ...
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The Effect of Macromolecule Source and Type of Media During in vitro Maturation of Sheep Oocytes on Subsequent Embryo Development
... In vitro culture: After IVF, presumptive zygotes were vortexed for 2–3 min to remove the cumulus cells and then washed in H-SOF to remove sperm- atozoa and cellular debris. They were then allo- cated to 20 µL drop of ...
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Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells
... 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and 100 U/mL penicillin/streptomycin (HyClone, NY, USA), and maintained at 37 °C in a humidified at- mosphere with 95% air and 5% CO ...
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RANK/RANKL/OPG system in the intervertebral disc
... endplate; FBS: Fetal bovine serum; Fisher PLSD test: Fisher protected least significant difference test; HE: Hematoxylin and eosin; HRP: Horseradish peroxidase; iAF: Inner annulus fibrosis; ...
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Proteasome inhibitors act as bifunctional antagonists of human immunodeficiency virus type 1 latency and replication
... ELISA: Enzyme-linked immunosorbent assay; Env: Envelope; FBS: Fetal bovine serum; FDA: Food and drug administration; FITC: Fluorescein isothiocyanate; GAPDH: Glyceraldehyde 3-phosphate d[r] ...
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The Apoptosis Effect on Liver Cancer Cells of Gold Nanoparticles Modified with Lithocholic Acid
... Abbreviations AuNPs: Gold nanoparticles; DMF: Dimethylformamide; EDC: 1-3Dimethylaminopropyl-3-ethylcarbodiimide hydrochloride; FBS: Fetal bovine serum; H2DCF-DA: 2′,7′-Dichlorofluoresce[r] ...
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Sargassum horneri (Turner) C Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO 1 signaling in RAW 264 7 cells
... Abbreviations ARE: Antioxidant response element; COX-2: Cyclooxygenase-2; DMEM: Dulbecco’s modified Eagle’s medium; ELISA: Enzyme linked immunosorbent assay; FBS: Fetal bovine serum; FD:[r] ...
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The role of microglia and the TLR4 pathway in neuronal apoptosis and vasospasm after subarachnoid hemorrhage
... sulfoxide; FBS: Fetal bovine serum; ICV: Intracerebroventricular; IRAK4: IL-1 receptor associated kinase 4; LPS: Lipopolysaccharide; MCA: Middle cerebral artery; MCSF: Macrophage ...
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Circulating Magnetic Microbubbles for Localized Real-Time Control of Drug Delivery by Ultrasonography-Guided Magnetic Targeting and Ultrasound
... repetition frequency; PNP: peak negative pressure; PBS: phosphate buffered saline; FBS: fetal bovine serum; LL/2: Lewis lung carcinoma; EDTA: ethylenediaminetetraacetic acid; DC: dire[r] ...
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The omega-3 polyunsaturated fatty acid docosahexaenoic acid inhibits proliferation and progression of non-small cell lung cancer cells through the reactive oxygen species-mediated inactivation of the PI3K /Akt pathway
... acid; FBS: Fetal bovine serum; FITC: Fluorescein isothiocyanate; FO: Dietary fish oil; HRP: Horseradish peroxidase; MMP9: Matrix metallopeptidase; MTT: 3-(4,5-dimethylthiazol-2-yl)- ...
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Histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), enhances anti tumor effects of the poly (ADP ribose) polymerase (PARP) inhibitor olaparib in triple negative breast cancer cells
... Abbreviations CI: combination index; DSB: double-strand break; FACS: fluorescenceactivated cell sorting; FBS: fetal bovine serum; H&E: hematoxylin and eosin; HDAC: histone deacetylase; H[r] ...
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Harvesting multipotent progenitor cells from a small sample of tonsillar biopsy for clinical applications
... BM: Bone marrow; BSA: Bovine serum albumin; CM: Complete medium; DMEM: Dulbecco ’ s modified Eagle ’ s medium; DPBS: Dulbecco ’ s phosphate-buffered saline; FBS: Fetal bovine serum; GFP:[r] ...
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Effects of the epigenetic drug MS-275 on the release and function of exosome-related immune molecules in hepatocellular carcinoma cells
... medium; FBS: Fetal bovine serum; FCS: Fetal calf serum; HDACi: histone deacetylase inhibitor; HepG2: Hepatoma G2; HSP: heat shock protein; IL: Interleukin; MHC: Major ...
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Tumor vascular permeability factor stimulates endothelial cell growth and angiogenesis
... promoted the growth of new blood vessels when administered into healing rabbit bone grafts or rat corneas. The identity of the growth factor activity with VPF was established in four ways: (a) the molecular weight of the ...
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Molecular characterization of a 31 kDa protein from Trichinella spiralis and its induced immune protection in BALB/c mice
... anti-rTs31 serum on larval invasion. Infection serum (IS, 1:50 dilution) and pre-immune serum (PI, 1:50) were used as positive and negative controls, ...
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