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Flow and mass cytometry analysis

A novel statistical analysis and interpretation of flow cytometry data

A novel statistical analysis and interpretation of flow cytometry data

... In this document, we have described and analyzed a recent class of mathematical models which combines the cyton model of population generation structure with a mass-conservation model of label dynamics. Unlike ...

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Cytofast: A workflow for visual and quantitative analysis of flow andmass cytometry data to discover immune signatures and correlations

Cytofast: A workflow for visual and quantitative analysis of flow andmass cytometry data to discover immune signatures and correlations

... and mass cytometry allows exceptional high-resolution exploration of the cellular compo- sition of the immune ...generated. Analysis frameworks such as FlowSOM or Cytosplore incorporate clustering ...

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Identification of mesenchymal stem cells and osteogenic factors in bone marrow aspirate and peripheral blood for spinal fusion by flow cytometry and proteomic analysis

Identification of mesenchymal stem cells and osteogenic factors in bone marrow aspirate and peripheral blood for spinal fusion by flow cytometry and proteomic analysis

... Switzerland) at 60°C for 30 min and alkylated with 55 mM iodoacetamide (Amersham Biosciences, Amersham, Buckinghamshire, UK) at room temperature for 30 min. After reduction and alkylation, the proteins were digested with ...

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Human bone marrow assessment by single-cell RNA sequencing, mass cytometry, and flow cytometry

Human bone marrow assessment by single-cell RNA sequencing, mass cytometry, and flow cytometry

... Paired analysis of the same sample by both transcriptome and cell surface phenotype offers a pow- erful opportunity to compare cell population frequencies determined by these ...and flow cytometry in ...

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Usefulness of Immuno Magnetic Beads Conjugated with Anti EpCAM Antibody for Detecting Endometrial  Cancer Cells

Usefulness of Immuno Magnetic Beads Conjugated with Anti EpCAM Antibody for Detecting Endometrial Cancer Cells

... with flow cytometry analysis were 73% and 75%, ...imaging cytometry analysis were 46% and 81%, ...cellular analysis was ...

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Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages.

Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages.

... For an investigator seeking to make their own fluorescently tagged strains a number of factors must be considered once stable transformants have been produced to ensure valid results in the flow cytometry ...

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Bone marrow cell homing to sites of acute tibial fracture: 89Zr-oxine cell labeling with positron emission tomographic imaging in a mouse model

Bone marrow cell homing to sites of acute tibial fracture: 89Zr-oxine cell labeling with positron emission tomographic imaging in a mouse model

... by flow cytometry and ...groups). Flow cytometry analysis of donor cells collected from the injured site revealed a predominance of CD45 + stem/progenitor cell populations and ...

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Launch of “Flow Focus” – Clinical flow cytometry e newsletter!

Launch of “Flow Focus” – Clinical flow cytometry e newsletter!

... 1) Diagnosis of Lymphomas: One of the mainstays for establishing the diagnosis of lymphoma is to demonstrate clonality. Clonality in B-NHL is usually done by evaluating the expression of immunoglobulin kappa ( κ ) and ...

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Original Article Knockdown of GOLM1 suppresses proliferation and aggressiveness of non-small-cell carcinoma cells

Original Article Knockdown of GOLM1 suppresses proliferation and aggressiveness of non-small-cell carcinoma cells

... Human NSCLC cell lines and EBAS-2B were pur- chased from Cell Bank of Chinese Academy of Science (Shanghai, China). Cell lines were cul- tured in Dulbecco’s Modified Eagle Medium (DMEM) or 1640 supplemented with 10% ...

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New horizons in platelets flow cytometry

New horizons in platelets flow cytometry

... Suitable and appropriate use of anticoagulant is mandatory for the processing of samples for flow cytometry. Sodium citrate is the anticoagulant of choice for platelet flow cytometric analysis ...

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THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH

THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH

... This technique is unquestionably of a great importance in the diagnosis of lypmhoma and leu- kemia. Cell subpopulations are characterised in detail and classified based on cell differentiation, surface and cytoplamsatic ...

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Probing the Cell Cycle with Flow Cytometry

Probing the Cell Cycle with Flow Cytometry

... the flow cytometer and the emitted fluorescent signal yields an electronic pulse with a height (amplitude) proportional to the total fluorescence emission from the ...of flow cytometric DNA analysis ...

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guava Flow Cytometry easycyte Systems

guava Flow Cytometry easycyte Systems

... easyCyte flow cytometry systems are uncomplicated instruments that deliver complex cell analysis — right on your ...of flow cytometry expertise, these instruments use smaller sample, ...

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Polychromatic flow cytometry is more sensitive than microscopy in detecting small monoclonal plasma cell populations

Polychromatic flow cytometry is more sensitive than microscopy in detecting small monoclonal plasma cell populations

... database included FC (enumeration and immunophenotype), aspirate and trephine microscopy, trephine immunohistochemistry, and immunology (serum and urine electrophoresis (EPG) and immunofixation (IFX)). The vast majority ...

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Original Article 3-cinnamoyl-4-hydroxy-6-methyl-2H-pyran-2-one (CHP) inhibits human ovarian cell proliferation by inducing apoptosis

Original Article 3-cinnamoyl-4-hydroxy-6-methyl-2H-pyran-2-one (CHP) inhibits human ovarian cell proliferation by inducing apoptosis

... FACSCalibur™ flow cytome- ter (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) was used for detection of apop- totic ...and analysis was performed using Modfit and Cell Quest™ software (Becton, ...

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Flow cytometry for intracellular SPION quantification: specificity and sensitivity in comparison with spectroscopic methods

Flow cytometry for intracellular SPION quantification: specificity and sensitivity in comparison with spectroscopic methods

... the flow cytometric measurements after gating on phenotypically healthy cells, characterized by Annexin V-negative and propidium iodide- negative ...Thus, flow cytometry provides relative cell counts ...

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HDAC2 depletion promotes osteosarcoma’s stemness both in vitro and in vivo: a study on a putative new target for CSCs directed therapy

HDAC2 depletion promotes osteosarcoma’s stemness both in vitro and in vivo: a study on a putative new target for CSCs directed therapy

... Methods: Saos2 and MG63 cells were treated with DAC and VPA, alone and in combination. Untreated and treated cells were examined for stemness phenotype by cytometry and real-time PCR. Sarcospheres and colonies ...

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Measuring DNA content in live cells by fluorescence microscopy

Measuring DNA content in live cells by fluorescence microscopy

... by flow cytometry. The analysis of 10,000 cells by flow cytometry yielded a distribution of ...by flow cytometry, with notice- ably underrepresented proportions of cells ...

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A measure of endosomal pH by flow cytometry in Dictyostelium

A measure of endosomal pH by flow cytometry in Dictyostelium

... cells to internalize both Oregon green-coupled dextran and Alexa 647-coupled dextran, the fluorescence of which is insensitive to pH (see Additional file 1). Oregon green and Alexa 647 fluorescence are perfectly ...

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Prevention of in vitro neutrophil endothelial attachment through shedding of L selectin by nonsteroidal antiinflammatory drugs

Prevention of in vitro neutrophil endothelial attachment through shedding of L selectin by nonsteroidal antiinflammatory drugs

... Flow cytometry analysis of neutrophils treated with indomethacin, diclofenac, piroxicam, ketoprofen, and aspirin in the same conditions than the adhesion experiments, showed that the cel[r] ...

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