Flow Cytometry assay for Biological Testing
Image cytometry as an alternative to flow cytometry for the transplant histocompatibility crossmatch assay
... compatibility assay performed by histocompatibility laboratories for transplant ...the flow cytometry crossmatch (FCXM) is the method of choice because of its superior sensitivity and ...a ...
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Flow Cytometry Based Assay for Titrating Dengue Virus
... Any assay for titrating a virus is a relative measure of infec- tious virus that is defined by properties of the particular virus and the methods ...plaque assay, which has been the standard method for ...
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Flow Cytometry Based Cytotoxicity and Antibody Binding Assay
... a flow cytome- ter equipped with a red laser (HeNe ∼630 to 640 nm) is preferred, there are alternative far- red excited dyes ...given flow cytometry ...
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Evaluation of flow cytometry for the detection of bacteria in biological fluids
... Statistical analysis Statistical analysis was performed using IBM SPSS Statistics 22.0 (Armonk, New York). Clini- cal and microbiological data were obtained from medical records in a fully anonymized and de-identified ...
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Rapid susceptibility testing of Candida albicans by flow cytometry
... using flow cytometry for the assessment of susceptibility test results is its ability to distinguish between viable and nonviable organ- ...by flow cytometry, which increases the chances that ...
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Application of flow cytometry in ballast water analysis - biological aspects
... Secondly, a compliance method must be able to detect the viability of cells. Often, fluorescent stains are used to evaluate cell vitality. Table 1 gives an overview of some available dyes to stain intact cells, dead ...
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Experimental and biological variability in CFSE-based flow cytometry data
... , and concluded that none of these schemes proved to be universally beneficial. In fact, in many cases these approaches tended to increase variability in parameter estimates. We also implemented a variation of our basic ...
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Rapid susceptibility testing for nontuberculosis mycobacteria using flow cytometry
... by flow cytometry in the present study, is that concentration of antimycobacterial agent which yielded a reduction in the mean channel fluorescence of at least 50% compared with the drug- free control ...
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Rapid susceptibility testing of Mycobacterium tuberculosis (H37Ra) by flow cytometry
... Several rapid methods for determining the resistance or susceptibility of M. tuberculosis have been reported (6, 15, 16, 19). A period of 7 to 12 days, however, is required for multi- plication of M. tuberculosis in ...
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Rapid whole-blood flow cytometry assay for diagnosis of chronic granulomatous disease.
... Chronic granulomatous disease (CGD) is characterized by defective killing of intracellular microorganisms due to mutations in one of the four known components of the NADPH oxidase system. This system is responsible for ...
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Rapid Whole-Blood Flow Cytometry Assay for Diagnosis of Chronic Granulomatous Disease
... Flow cytometry analysis. Following fixation, the samples were acquired on a FACScan flow cytometer (Becton Dickinson, Mountain View, Calif.). The ma- chine is equipped with an argon ion air-cooled ...
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Rapid susceptibility testing of fungi by flow cytometry using vital staining
... 1-h assay for antifungal susceptibility testing measuring the impairment of fungal metabolic activity was ...by flow cytometry with a novel fluorescent probe, FUN-1, which emits a red ...
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Antifungal Susceptibility Testing of Fluconazole by Flow Cytometry Correlates with Clinical Outcome
... 20. Ramani, R., A. Ramani, and S. J. Wong. 1997. Rapid flow cytometric sus- ceptibility testing of Candida albicans. J. Clin. Microbiol. 35:2320–2324. 21. Rex, J. H., J. E. Bennett, A. M. Sugar, P. G. ...
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Flow Cytometry Protocols
... have also been recently reviewed (34). The use of a single plate throughout the entire assay also avoids cell transfers that can be time consuming and contribute to loss of cells. Many variations in reagents for ...
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Flow Cytometry Protocols
... SP assay with violet excitation, relatively high laser power is required to produce discernible SP profiles of slightly lower resolution than those gener- ated by the standard method (compare ...cuvet flow ...
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flow cytometry reinvented
... With the Attune ® cytometer, even dilute samples can be acquired quickly without compromising data. Difficult-to-collect samples like mouse blood and bone marrow, thin-needle aspirates, or any sample with low cell yield ...
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Flow Cytometry Protocols
... Release Assay This assay has been shown to provide a single-cell-based, rapid, quantitative, and sensitive assay to detect lymphocyte-mediated target cell killing in various animal models ...FCC ...
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A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood
... novel assay for detection of Staphylococcus in blood culture samples, which combines the advantages of a phage endolysin cell wall binding domain (CBD) as a specific probe with the accuracy and high-throughput of ...
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FASTIMMUNE Assay System A Rapid and Comprehensive System for Assessing Lymphocyte Function by Flow Cytometry
... unique assay system that directly assesses individual T-lymphocyte subset responses to various physiological and non-physiological ...The assay can be completed within 5 hours in whole blood without tissue ...
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Micronuclei detection by flow cytometry as a high-throughput approach for the genotoxicity testing of nanomaterials
... the biological effects associated with those NMs being under development or already present in the ...a flow cytometry-based approach testing micronucleus (MNs) induction (FCMN ...FCMN ...
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