Flt3-L
Attenuation of vaccinia virus by the expression of human Flt3 ligand
... Then we administered these viruses intraperitoneally to 6-weeks-old mice and measured the expression of FL in vivo for several days after inoculation. The animals inocu- lated with P13-E/L-FL did not have highly ...
15
Viral targeting of hematopoietic progenitors and inhibition of DC maturation as a dual strategy for immune subversion
... fewer than 10% of BMCs were infected (i.e., day 5 after infection), an inhibitory effect on DC development was noted (see Figure 2). Thus, Cl 13 may have the capacity to impair the expansion of progenitor cells by two ...
10
Targeting FLT3 in acute myeloid leukemia using ligand-based chimeric antigen receptor-engineered T cells
... target FLT3 using its ligand as recognizing domain, the FLT3L CAR, was ...both FLT3 + leukemia cell lines and primary AML cells at the E:T ratio as low as ...of FLT3 expression level on leukemia ...
12
Activation of protein phosphatase 2A in FLT3+ acute myeloid leukemia cells enhances the cytotoxicity of FLT3 tyrosine kinase inhibitors
... (D) FLT3 was isolated from BaF3/FLT3-ITD cells by immunoprecipitation followed by western blot after treatment with FTY720 (3 µM), AAL(S) (3 µM) or CEP701 (5 nM) for 24 ...total FLT3 bands, and shown ...
14
Reindl, Carola (2007): Aberrante Aktivierung der Rezeptortyrosinkinase FLT3 in der akuten myeloischen Leukämie. Dissertation, LMU München: Medizinische Fakultät
... RTK FLT3 konstitutiv aktiviert ist (Spiekermann, ...gewonnenen FLT3 cDNA zeigte eine Punktmutation in der JM-Domäne: Substitution von Valin durch Alanin an Position 592 ...in FLT3 identifizieren: ...
129
FLT3 inhibitors: clinical potential in acute myeloid leukemia
... 1) FLT3-ITD receptors are efficiently targeted by TKI (T) but bypass activation of oncogenic tyrosine kinases (SYK or AXL) or serine/threonine kinases (Pim) reduce the activity of TKi and enhance AML cell ...
9
FLT3 Mutations: Significance in Paediatric AML
... the FLT3 gene ...[10]. FLT3 receptor is composed of juxtamembrane dimerization domain, transmembrane domain, extracellular domain and intracellular kinase domain ...Initially, FLT3 is present in a ...
7
FLT3 inhibitors in acute myeloid leukemia
... both FLT3/ITD and re- sistant FLT3/D835 mutants in patients with AML ...diagnosed FLT3 mutant AML ...with FLT3/ITD and 3 patients with FLT3/D835 ...with FLT3-mutated AML ...
11
The MERTK/FLT3 inhibitor MRX-2843 overcomes resistance-conferring FLT3 mutations in acute myeloid leukemia
... to FLT3 inhibition, the effects of inhibitors such as quizartinib have been well described, with myelosuppression being one of the most prominent toxicities, likely due to the com- bined inhibition of both ...
17
SU11652 Inhibits tyrosine kinase activity of FLT3 and growth of MV-4-11 cells
... The FLT3 substrate GST fusion protein GST-FLT3S was purified from ...type FLT3 and its D835H and D835Y mu- tant forms were isolated from recombinant baculovirus- infected Sf9 insect cells by using the ...
6
Expression of GADS enhances FLT3-induced mitogenic signaling
... [37–39]. FLT3 has several such phosphotyrosine ...in FLT3 that interact with GADS, we used synthetic tyrosine phosphorylated peptides corresponding to known or predicted tyrosine phosphorylation sites in ...
13
Frequency of FLT3 ITD and FLT3 TKD Mutations in Multiple Myeloma Patients (A Case Control Study)
... 7]. FLT3 belongs to class III tyrosine kinase receptor family [4], and is made up of five extracellular immunoglobulin-like domains, a short juxtamembrane intracellular segment and a transmembrane portion [7, ...
7
Abundance of Flt3 and its ligand in astrocytic tumors
... samples. Flt3 expression could generally be demonstrated by 40 specific polymerase chain reaction cycles and gel electrophoresis in 87% of the tumors, including all grades, although the small quantities of the ...
7
Clinical use of FLT3 inhibitors in acute myeloid leukemia
... Midostaurin is also a multikinase inhibitor, originally developed to target protein kinase C. It was also found to inhibit wild-type and mutant (both ITD and TKD) FLT3, PDGFR- α and β , VEGFR-2, and KIT (wild-type ...
12
Studies of FLT3 mutations in paired presentation and relapse samples from patients with acute myeloid leukemia: implications for the role of FLT3 mutations in leukemogenesis, minimal residual disease detection, and possible therapy with FLT3 inhibitors
... Five patients (25%) lost their FLT3 mutation at relapse. All had presented with an ITD, and the mutant levels were 6%, 11%, 20%, 28%, and 44%. Polymorphism analysis demonstrated that the paired samples were from ...
7
Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia
... was knocked down using ectopic short hairpin RNA (shRNA) in MV4-11 cells. As shown in Figure 3A, the five tested shRNAs regulated the CD97 expression with different efficiency. The empty plko.1 vector served as control. ...
12
FLT3 in lineage specification and plasticity
... differentiation. FLT3 expression in Pax5 negative lymphoid precursors coincides with a window of multilineage differentiation potential in mice and ...that FLT3 activating mutations can collaborate with a ...
5
Co-expression of wild-type FLT3 attenuates the inhibitory effect of FLT3 inhibitor on FLT3 mutated leukemia cells
... (KD-) FLT3 (K644A) was generated using PrimeSTAR Mutagenesis Basal Kit (Takara Bio ...of FLT3-expressing 32D cells: sole Wt-FLT3 expressing cells, ITD-FLT3-Myc and ITD-FLT3-FLAG ...
15
Melatonin enhances sorafenib-induced cytotoxicity in FLT3-ITD acute myeloid leukemia cells by redox modification
... Melatonin is characterized with pleiotropic activities, such as physiological circadian regulation and antitumor properties [39]. Melatonin has been reported to enhance the antitumor activity of multiple chemotherapeutic ...
12
Cytogenetic and FMS-Like Tyrosine Kinase 3 Mutation Analyses in Acute Promyelocytic Leukemia Patients
... acquisition of additional oncogenic lesions [5]. There has also been considerable interest in the potential role of activating mutations of genes encoding receptor tyrosine kinases, which commonly accompany acute ...
8