Fluorescence in Situ Hybridisation
Detection of abl/bcr Fusion Gene in Patients Affected by Chronic Myeloid Leukaemia by Dual-Colour Interphase Fluorescence in situ Hybridisation
... Interphase fluorescence in situ hybridisation (FISH) is a technique that enables rapid detection of chromosomal rearrangements on interphase cells, avoiding the requirement for metaphase cell ...
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Detection of various U and M chromosomes in wheat Aegilops biuncialis hybrids and derivatives using fluorescence in situ hybridisation and molecular markers
... Fluorescence in situ hybridisation was used to determine the karyotypic constitution of four wheat-Ae. biuncialis addition lines and two other promising lines, both containing three differ- ent Ae. ...
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Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
... in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained ...
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The use of fluorescence in situ hybridisation techniques in the diagnosis and prognosis of malignancy
... affecting fluorescence in situ hybridisation (FISH) at that ...d fluorescence is red u ced , and sen sitiv ity an d re so lu tio n are enhanced (Lawrence, ...of hybridisation to unique ...
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First study of pathogen load and localisation of ovine footrot using fluorescence in situ hybridisation (FISH)
... in situ hybridisation (FISH) can be used to detect bacteria in their natural environment, providing information regard- ing bacterial load and localisation (Baumgart et ...
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Investigation of chromosomal abnormalities during colorectal tumour development by somatic cell hybrid and fluorescence in-situ hybridisation analysis
... Mutations in oncogenes were originally detected by focus formation in NIH3T3 cells, which suggested the presence of activated ras oncogenes in 10-15% of human tumours (Pulciani et al. 1982). The development of more ...
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Karyotype analysis of Lablab purpureus (L ) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes
... Mitotic chromosomes and interphase nuclei of Lablab purpureus after sequential combined propidium iodide and 4',6-diamidino-2-phenylindole CPD staining and FISH with 5S and 45S rDNA prob[r] ...
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A fluorescence in-situ hybridisation and molecular investigation of leukaemia associated abnormalities of chromosome 6q.
... GluR-6 through this mechanism occurs in ALL, sequence analysis of the GluR-6 exons and flanking intronic sequences was performed in patients and cell lines known to bear deletions [r] ...
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Cryopreservation and genetic damage : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Genetics at Massey University
... In this study three cytogenetic tests: Sister Chromatid Exchange SCE, Micronucleus Assay MN and Fluorescence in situ Hybridisation FISH were conducted on whole blood samples from ten par[r] ...
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Is There any Concordance Between of IHC with FISH in HER2-Positive Breast Cancer Patients?
... by fluorescence in situ hybridization and immunohistochemistry in a single institution over 12 years: a quality control ...and fluorescence in situ hybridisation ...
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DNA Copy Number Variation in a Cohort of Healthy Australian Women
... genomic hybridisation (CGH), first developed in 1993 (58) provided the foundation for future technological ...using fluorescence in- situ hybridisation (FISH) which are applied to metaphase ...
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Cryopreservation and genetic damage : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Genetics at Massey University
... That Sister Chromatid Exchange, Micronucleus Assay and Fluorescence in situ Hybridisation can be performed on whole blood of radiation exposed veterans and non-exposed controls after a p[r] ...
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Comparative cytogenetics in the genus Trifolium section Trifolium (clover) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Plant Biology at Massey University
... This is the first time any species in the genus Trifolium section Trifolium have been successfully C-banded, Q-banded, and subjected to fluorescence in situ hybridisation.... iii The inf[r] ...
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Abstracts of the 12th European Cytogenomics Conference 2019
... segments. Fluorescence in-situ hybridisation (FISH), in particular fibre-FISH, remains the current method of choice for characterising such complex genomic structure and variation, since it enables ...
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Fluorescence in situ hybridization as adjunct to cytology improves the diagnosis and directs estimation of prognosis of malignant pleural effusions
... Molecular analysis of the pleural fluid of patients using fluorescence in situ hybridisation to discriminate those with benign and malignant pleural effusions has not been well studied. We ...
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Disomy 21 in spermatozoa and the paternal origin of trisomy 21 Down syndrome
... the two chromosomes 21 at the first meiotic division, taking place just before ovulation (see e.g. Table one in [2], [3–5]). In sharp contrast, we have concluded that the majority, if not all cases of T21, having an ...
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Array comparative genomic hybridisation based identification of two imbalances of chromosome 1p in a 9 year old girl with a monosomy 1p36 related phenotype and a family history of learning difficulties: a case report
... 22q11 fluorescence in situ hybridisation (FISH) analysis and routine cytogenetic investigations, both of which revealed an apparently nor- mal female karyotype (46, ...
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QUANTIFICATION OF BIOFILMS IN MULTI-SPECTRAL DIGITAL1 VOLUMES FROM CONFOCAL LASER-SCANNING MICROSCOPES
... by fluorescence marked with fluorescent probes are digitised with a confocal laser scanning ...in situ hybridisation, green fluorescent protein, confocal laser-scanning microscopy, mathematical ...
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Chromogenic in situ hybridisation for the assessment of HER2 status in breast cancer: an international validation ring study
... categorises tumours as 3+ (strongly positive staining), 2+ (moderately strong staining), 1+ (weak staining) and 0 (negative for staining). An IHC score of 3+ is classified as positive for HER2 overexpression, but a ...
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Comparison of different commercial kits for HER2 testing in breast cancer: looking for the accurate cutoff for amplification
... available fluorescence/ chromogenic in situ hybridisation (FISH/CISH) kits and validate one for the resolution of borderline immunohistochemistry (IHC) ...
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