fluorescence lifetime imaging
Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy
... with fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) and show for the first time that AcMNPV capsid proteins VP39 and EXON0, but not Orf1629, ...
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Towards unsupervised fluorescence lifetime imaging using low dimensional variable projection
... large fluorescence lifetime imaging (FLIM) data is becoming overwhelming; the latest FLIM systems easily produce massive amounts of data, making an efficient analysis more challenging than ...the ...
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GPU acceleration of time-domain fluorescence lifetime imaging
... Abstract. Fluorescence lifetime imaging microscopy (FLIM) plays a significant role in biological sciences, chem- istry, and medical ...up lifetime calculations compared to CPU-OpenMP (parallel ...
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Single-shot time-gated fluorescence lifetime imaging using three-frame images
... single-shot fluorescence lifetime imaging (FLI) technology with high ...time-gated fluorescence lifetime imaging using three-frame images (TFI-TGFLI), is ...rapid lifetime ...
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Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches
... Time-correlated single photon counting (TCSPC) systems are widely as the gold standard fluorescence lifetime imaging (FLIM) owing to high temporal resolution. The acquisition speed has been ...
5
Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... steady-state fluorescence intensity still suffered from artifacts arising from the heterogeneity of tissue optical properties [8], local environment, unknown chromophores with over- lapped spectra, and the ...
10
Fluorescence lifetime imaging of E combretastatin uptake and distribution in live mammalian cells
... using fluorescence lifetime imaging, in which the rastered excitation laser beam dwells on each pixel for a few milliseconds and at each position records a nanosecond fluorescence ...
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Mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy
... and fluorescence lifetime imaging microscopy (FLIM) are able to provide important information about the concen- tration and the spatial distribution of a fluorophore within a sample, since this ...
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Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells
... Results: Fluorescence lifetime imaging microscopy (FLIM) can be is used to determine intra- or intermolecular interactions by fluorescently labeling epitopes on one or two different ...fluorophore ...
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Using in vivo fluorescence lifetime imaging to detect HER2-positive tumors
... Fluorescence lifetime imaging is based on evaluation of the average time that electronically excited fluoro- phore stays in that excited state before its transition to a ground state, accompanied by ...
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Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging
... excited fluorescence lifetime imaging ...the fluorescence lifetime of fluorophore and the refractive index of its microen- vironment varying due to changes in the concentrations of ...
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Molecular rheometry : direct determination of viscosity in Lo and Ld lipid phases via fluorescence lifetime imaging
... The laser light was coupled to the single mode optical fiber (in which polarisation was maintained) and re-collimated at the output with an UPlanSApo 10 N.A. 0.4 objective lens (Olympus) so that the beam light ...
8
Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... Co-localization of Vpr-eGFP and either mCherry-Vpr or Vpr-mCherry was visualized by confocal microscopy. As a control, Vpr-eGFP was first co-expressed with mCherry. Localization of Vpr-eGFP at the nuclear rim (Figure 3, ...
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A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging
... generated fluorescence in the microscope focal plane via a two photon absorption process and was back-projected through the system to the detection ...each fluorescence image of the beamlet array was ...
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Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays
... Both time-domain and frequency-domain instrumentation systems are available to acquire FLIM data. Here, we will focus on the recently proposed time-domain approaches and discuss how they can be applied to the latest ...
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Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... on fluorescence or Raman spectroscopy [4,5]. Fluorescence methods have intrinsically high sensitivity with the ability to report on molecular processes and environmental effects [6] and depend either on the ...
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Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells
... FLIM: fluorescence lifetime imaging microscopy; GCTOFMS: gas chromatography time-of-flight mass spectrometry; GSH: reduced glutathione; GSSG: glutathione disulfide, oxidized glutathione; ...
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Homomultimerization of the Coxsackievirus 2B Protein in Living Cells Visualized by Fluorescence Resonance Energy Transfer Microscopy
... by fluorescence resonance energy transfer (FRET) micros- ...spectral imaging microscopy (SPIM) and fluorescence lifetime imaging microscopy ...
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Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer
... Confocal fluorescence lifetime imaging (FLIM) was per- formed with a Microtime200 (Picoquant GmbH) or Alba (ISS, Champaign) scanning confocal time-resolved microscope ...histogram. ...
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The Effects of Extreme Media on Political Behavior, Attitudes, and Media Selection
... static fluorescence microscopy remain, including uneven dye loading, leakage and ...The fluorescence lifetime imaging (FLIM) overcomes these limitations with its advantage of being independent ...
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