Formalin-fixed paraffin-embedded tissues
Original Article Association between mir-24 and mir-378 in formalin-fixed paraffin-embedded tissues of breast cancer
... Abstract: Background: MiR-24/378 is thought to be onco-miRNAs for their ability of enhancing tumor growth. The objective of this study was to evaluate the potential predictive value of miR-24/378 expression in ...
7
Evaluation of PCR in Detection of Mycobacterium tuberculosis from Formalin Fixed, Paraffin Embedded Tissues: Comparison of Four Amplification Assays
... from formalin-fixed, paraffin-embedded ...from paraffin-embedded tissues and can be particularly useful in the validation of a diagnosis of tuberculosis in clinical ...
6
Molecular characterization of high risk human papillomavirus genotypes involved in invasive cervical cancer from formalin fixed, paraffin embedded tissues in Ouagadougou, Burkina faso
... made with an excess of paraffin. To confirm the negative impact of paraffin, Steinau et al. had compared the dewaxing method used in our study with another method using heat (Steinau et al., 2011). He ...
5
Whole genome sequencing of genotype VI Newcastle disease viruses from formalin fixed paraffin embedded tissues from wild pigeons reveals continuous evolution and previously unrecognized genetic diversity in the U S
... The evolutionary distances between the U.S. pigeon se- quences and other genotype VI viruses were estimated. Seventeen sequences obtained in the current study from tissues sampled between 2014 and 2016 from Texas, ...
11
Original Article MicroRNA Expression Profiling Outperforms mRNA Expression Profiling in Formalin-fixed Paraffin-embedded Tissues
... of formalin fixation show that the principle change includes the addition of a hydroxymethyl group (termed “methylol”) to the nucleic acid ...time embedded in paraffin ...after formalin ...
9
An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays
... After establishing that method 4 exhibited the highest pro- tein yields and showed only partial protein degradation on Coomassie staining and western blots, we proceeded with RPPA, in order to evaluate protein expression ...
12
Efficient extraction of proteins from formalin-fixed paraffin-embedded tissues requires higher concentration of tris(hydroxymethyl)aminomethane
... liver tissues obtained from male C57BL/6 ...Center. Tissues were fixed in 4% buffered formalin for 48 h, dehydrated with a graded series of ethanol before being embedded in ...
6
Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
... FFPE: Formalin-fixed paraffin-embedded; FF: Fresh-frozen; GCRMA: GC robust multiarray average; DLDA: Diagonal linear discriminant analysis; LOOCV: Leave-one-out cross-validation; CA4: Carbonic ...
10
Detection of Survivin Gene Expression in Formalin-Fixed Paraffin-Embedded Tissues of Human Osteosarcoma: Its Potential Usefulness in Diagnosis and Prognosis of Bone Tumors
... Biopsy patients (66.6%) showed detectable expression of survivin. These are patients that haven’t undergone any therapy procedures and referred to institute for first time of their disease. Three biopsy samples were ...
7
Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands
... FFPE tissues has been reported ...by formalin fixation, several studies have been conducted to test the applicability of other fixatives to proteomic analysis ...Fine-FIX fixed [3] and ...
10
Duplex PCR for Differential Identification of Mycobacterium bovis, M avium, and M avium subsp paratuberculosis in Formalin Fixed Paraffin Embedded Tissues from Cattle
... Members of our group, along with others, have previous- ly isolated two DNA segments of M. avium subsp. paratuber- culosis: the p34 gene, coding for a 34-kDa mycobacterial antigenic protein (3), and the f57 segment (23). ...
7
Detection and identification of mycobacteria in formalin fixed, paraffin embedded tissues by nested PCR and restriction enzyme analysis
... A novel assay based on a nested PCR and restriction enzyme analysis of the PCR products was developed for the rapid detection and identification of Mycobacterium bovis and M. avium-M. intracellulare species in ...
5
CD68, CD163, and matrix metalloproteinase 9 (MMP 9) co localization in breast tumor microenvironment predicts survival differently in ER positive and negative cancers
... in formalin-fixed paraffin-embedded tissues of breast carcinomas represented in tissue microarray format using multiplexed quantitative immunofluorescence (QIF) in two independent Yale ...
10
A proteomic profile of synoviocyte lesions microdissected from formalin-fixed paraffin-embedded synovial tissues of rheumatoid arthritis
... Mascot software (version 2.2.06, Matrix Science, Lon- don, UK) was used for database search against Homo sapiens entries in the UniProtKB/Swiss-Prot database (release 2012_02, 20413 entries). Peptide mass toler- ance was ...
10
Prevalence of human papillomavirus genotypes among women with cervical cancer in Ghana
... using formalin- embedded paraffin-embedded specimen (presence of inhibitors from sample fixatives), it was possible that the other multiple-infecting high risk HPV genotypes present were not ...
9
Use of uracil-DNA glycosylase enzyme to reduce DNA-related artifacts from formalin-fixed and paraffin-embedded tissues in diagnostic routine
... from formalin-fixed and paraffin-embedded (FFPE) tumor tissues, which can result in detection of nonreproducible sequence artifacts, especially C:G > T:A transitions, in ...
6
Validation of the MethylationEPIC BeadChip for fresh-frozen and formalin-fixed paraffin-embedded tumours
... First, we compared the β values for the ~425,000 CpG sites that are present on both the 450K and the EPIC array for nine FF samples, and three FFPE samples. DNA from the same batch was used for both array types. Density ...
6
miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples
... from formalin-fixed paraffin-embedded (FFPE) samples leading to the identification of miRNAs that could be developed as diagnostic and prognostic biomarkers, in particular those involved in ...
14
Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer
... paired formalin-fixed, paraffin-embedded (FFPE) specimens from primary CRC tissues and patient-matched normal colonic tissues were obtained from the 6 th Affiliated Hospital of ...
11
Original Article Quantitative RT-PCR assay of HER2 mRNA expression in formalin-fixed and paraffin-embedded breast cancer tissues
... remove paraffin from FFPE tissue samples, two pieces of 10-µm-thick FFPE breast cancer tissue sections were put into a ...the paraffin and centrifuged for 2 min at room temperature at 20,000 x g to ...
8