Fusion (F) protein
Identification of Domains on the Fusion (F) Protein Trimer That Influence the Hemagglutinin-Neuraminidase Specificity of the F Protein in Mediating Cell-Cell Fusion
... cell-cell fusion even in the absence of coexpressed HN ...the F protein of PIV5 strain W3A induces cell-cell fusion by itself (21), while the F proteins of most parainfluenza viruses, ...
9
Hemagglutinin-Neuraminidase-Independent Fusion Activity of Simian Virus 5 Fusion (F) Protein: Difference in Conformation between Fusogenic and Nonfusogenic F Proteins on the Cell Surface
... paramyxovirus F protein (1). The F protein is predicted to undergo confor- mational changes during the fusion process: formation of the trimeric coiled-coil structure of HR1, insertion ...
11
Residues of the Human Metapneumovirus Fusion (F) Protein Critical for Its Strain-Related Fusion Phenotype: Implications for the Virus Replication Cycle
... the F protein of certain hMPV strains in transfected cells was duplicated in cultures infected with the same viruses, suggest- ing that no other viral protein substantially influenced the extent of ...
12
Roles of the Putative Integrin-Binding Motif of the Human Metapneumovirus Fusion (F) Protein in Cell-Cell Fusion, Viral Infectivity, and Pathogenesis
... cell-cell fusion without sig- nificantly altering cell surface expression of F ...hMPV F protein to promote cell-cell ...the fusion activity of hMPV F ...
15
Amino Acid Substitutions in the F-Specific Domain in the Stalk of the Newcastle Disease Virus HN Protein Modulate Fusion and Interfere with Its Interaction with the F Protein
... (HN) protein of Newcastle disease virus mediates attachment to sialic acid receptors, as well as cleavage of the same ...the fusion (F) protein, to promote membrane ...impair fusion and ...
9
The Paramyxovirus Fusion Protein C-Terminal Region: Mutagenesis Indicates an Indivisible Protein Unit
... membrane. Fusion is mediated by the viral fusion (F) protein, and it undergoes large irreversible conformational changes to cause membrane ...PIV5 F contains a membrane-proximal ...
10
Decreased Dependence on Receptor Recognition for the Fusion Promotion Activity of L289A-Mutated Newcastle Disease Virus Fusion Protein Correlates with a Monoclonal Antibody-Detected Conformational Change
... (NDV) fusion (F) protein gains the ability to promote fusion of Cos-7 cells independent of the viral hemagglutinin-neuraminidase (HN) protein and exhibits a 50% enhancement in ...
11
The Human Metapneumovirus Fusion Protein Mediates Entry via an Interaction with RGD-Binding Integrins
... the fusion (F) protein. How HMPV F mediates both attachment and fusion has not been clearly ...that F mediates HMPV entry through an interaction with RGD-binding ...HMPV F ...
13
Addition of N-Glycans in the Stalk of the Newcastle Disease Virus HN Protein Blocks Its Interaction with the F Protein and Prevents Fusion
... its fusion-promoting ...in fusion, we introduced a single glycosylation site at a convenient position in the ...mutated protein relative to wt HN and comigration of the two proteins following PNGase ...
11
The F-Like Protein Ac23 Enhances the Infectivity of the Budded Virus of gp64-Null Autographa californica Multinucleocapsid Nucleopolyhedrovirus Pseudotyped with Baculovirus Envelope Fusion Protein F
... contain F-like pro- teins, such as those encoded by Ac23 from Autographa califor- nica multinucleocapsid NPV (AcMNPV) (7) and Op21 from Orgyia pseudotsugata MNPV ...These F-like proteins coexist with GP64 ...
5
Role of the Simian Virus 5 Fusion Protein N-Terminal Coiled-Coil Domain in Folding and Promotion of Membrane Fusion
... SV5 F protein (Fig. 4) and promoted fusion at a lower temperature than the wt F protein ...of fusion and fusion promotion at lower ...SV5 F protein six-helix ...
9
Five Residues in the Apical Loop of the Respiratory Syncytial Virus Fusion Protein F2 Subunit Are Critical for Its Fusion Activity
... mediates fusion at neutral pH (9). Paramyxovirus fusion proteins—such as those of parainfluenza virus 5 (PIV5), measles virus, and Nipah virus—interact with their homotypic viral attachment protein ...
16
Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein
... SeV F protein in which its cleavage site was mutated to R-Q-K-R (designated Fcl) (see Materials and Methods), which is similar to another SeV F protein con- struct that is intracellularly ...
12
The Respiratory Syncytial Virus Phosphoprotein, Matrix Protein, and Fusion Protein Carboxy-Terminal Domain Drive Efficient Filamentous Virus-Like Particle Formation
... (M) protein un- derlying the membrane and were often filamentous in nature ...matrix protein (M), and the fusion protein (F) were each neces- sary for packaging and passaging of a ...
17
Activation of Fusion by the SER Virus F Protein: a Low-pH-Dependent Paramyxovirus Entry Process
... virus F pro- tein requires additional energy for fusion activation of the metastable state, the effect of elevated temperature on the fusion activity of the SER virus F protein and ...
8
N-Glycans of F Protein Differentially Affect Fusion Activity of Human Respiratory Syncytial Virus
... had fusion characteristics similar to those of N500Q, whereas a lower fusion activity was found in the case of ...the F protein, only the N116Q and N120Q mutants exhibited fusion ...
8
Characterization of Human Metapneumovirus F Protein-Promoted Membrane Fusion: Critical Roles for Proteolytic Processing and Low pH
... HMPV F-promoted fusion. Fusion pro- moted by the HMPV F protein has yet to be examined in the absence of other viral ...HMPV F protein-promoted fusion, the HMPV ...
11
Insertion of the Two Cleavage Sites of the Respiratory Syncytial Virus Fusion Protein in Sendai Virus Fusion Protein Leads to Enhanced Cell-Cell Fusion and a Decreased Dependency on the HN Attachment Protein for Activity
... RSV F cleavage sites I and II in SeV F protein leads to enhanced membrane fusion in the presence of ...both F RSV cleavage sites I and II ...all F SeV chi- meras containing the ...
13
A Cellular Drosophila melanogaster Protein with Similarity to Baculovirus F Envelope Fusion Proteins
... cellular F pro- teins shows that both Dy-cF and Dp-cF have extended N- terminal sequences when compared with the Dm-cF sequence ...baculovirus F proteins, Dm-cF and the proteins encoded by the f ...
11
Triggering of Human Parainfluenza Virus 3 Fusion Protein (F) by the Hemagglutinin-Neuraminidase (HN) Protein: an HN Mutation Diminishes the Rate of F Activation and Fusion
... membrane fusion mediated by the fusion protein (F) has a stringent requirement for the presence of the homotypic hemagglutinin-neuraminidase protein ...the fusion process, we ...
8