γ-Tubulin
A novel resveratrol derivative induces mitotic arrest, centrosome fragmentation and cancer cell death by inhibiting γ-tubulin
... and then treated for 2 h. At the end of the treatment cells were processed as described in [7]. Primary antibod- ies were anti-α-tubulin and anti-γ-tubulin antibodies (Sigma-Aldrich). Secondary ...
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RacGAP50C directs perinuclear γ tubulin localization to organize the uniform microtubule array required for Drosophila myotube extension
... nucleator γ -tubulin changes from diffuse cytoplasmic staining in mononucleated myoblasts to discrete cytoplasmic puncta at the nuclear periphery in multinucleated myoblasts, and that this change in ...
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Functional Dissection of the γ-Tubulin Complex by Suppressor Analysis of gtb1 and alp4 Mutations in Schizosaccharomyces pombe
... 2001). Our suppressor screenings were not exhaustive and might be biased, particularly because in the second screening we selected only those with a sporulation- defective phenotype. Therefore, it is possible that there ...
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Shroom family proteins regulate γ tubulin distribution and microtubule architecture during epithelial cell shape change
... Whereas the molecular control of apical constriction has been at least partially defined (see below), the molecules governing apicobasal cell elongation remain almost entirely obscure. Electron microscopy and inhibitor ...
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Human Papillomavirus Type 16 E7 Oncoprotein Associates with the Centrosomal Component γ-Tubulin
... Several lines of evidence suggest that the association of HPV16 E7 with ␥ -tubulin may contribute to the pRb/p107/ p130-independent mechanism of induction of supernumerary centrosomes. Our findings that HPV16 E7 ...
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The role of fission yeast γ-tubulin interacting proteins in mitosis
... After the identification of the y-TuRC it was suggested that it forms a scaffold on which microtubules grow. Purification of similar y-tubulin complexes from Drosophila (Martin et a l, 1998) and human (Tassin et a ...
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p53 centrosomal localization diagnoses ataxia telangiectasia homozygotes and heterozygotes
... in 3.7% formaldehyde for 10 minutes at room temperature. Next, cells were permeabilized by a first incubation in 0.25% Triton X-100 in PBS (Lonza) for 5 minutes at room temperature, and a second incubation in 100% ...
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Impaired Ciliogenesis in differentiating human bronchial epithelia exposed to non-Cytotoxic doses of multi-walled carbon Nanotubes
... of γ -tubulin-staining basal bodies in treated cultures, MWCNTs may be disrupting early ciliogenesis events leading to ...as γ -tubulin staining of ciliary basal bodies is attenuated in ...
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New insights into subcomplex assembly and modifications of centrosomal proteins
... the γ-TuRC ...recruiting γ- TuRC to centrosomes ...the γ- TuRC, as it cofractionates with γ-Tubulin and GCP5 at a size expected for the γ-TuRC in sedimentation assays ...known ...
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Ciliogenesis is regulated by a huntingtin HAP1 PCM1 pathway and is altered in Huntington disease
... anti–N-acetyl tubulin (6-11B1, 1:200; Sigma-Aldrich), anti–γ-tubulin GTU88 (1:100; Sigma-Aldrich), and anti- GFAP (G3893, 1:1,000; Sigma-Aldrich); and the pAb anti–adenylyl cyclase III (1:4,000, ...
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Zoglmeier geb. Daunderer, Christine (2002): Characterizing Components of the Dictyostelium Centrosome. Dissertation, LMU München: Fakultät für Biologie
... centrosomes γ -tubulin has been shown to be present in large, ring-shaped complexes, which are anchored in the ...called γ TuRCs (γ-tubulin ring complexes), serve to nucleate ...
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Thesis
... transfected as indicated, immunoprecipitated for Axin RFP and blotted with anti-Flg, RFP, or γ -tubulin as a loading control. Samples were run on a single gel, extra lanes were re[r] ...
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HSP70 is required for the proper assembly of pericentriolar material and function of mitotic centrosomes
... and γ-tubulin, whose accumulation is also PLK1-dependent [15, ...of γ-tubulin has been implicated in abnormal spindle for- mation and human malignancies [22, ...and γ-tubulin ...
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The dual functions of α-tubulin acetylation in cellular apoptosis and autophage induced by tanespimycin in lung cancer cells
... α-Tubulin and Hsp90 are two main non-histone sub- strates of HDAC6. When HDAC6 is inhibited, the acetylation levels of α-tubulin and Hsp90 are upregu- lated and their normal physiological functions may be ...
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The electrostatic analysis of TOG domains from XMAP215/DIS1 family members
... pure tubulin is controlled by GTP hydrolysis and this modu- lates the size and distribution of the ...GDP.Pi tubulin (Bieling et ...of tubulin, based upon evidence of its ability of bind GTP ...
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Different Functionals Domain of SpastinM87V Affect Cellular Microtubule Cutting
... The effects of different functional domains of Spastin M87V on the microtubule cutting of Hela cells were investigated. Spastin is a microtubule-cutting protein that can participate in many physiological functions, such ...
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Herpes Simplex Virus Type 1 Tegument Protein VP22 Induces the Stabilization and Hyperacetylation of Microtubules
... acetylated tubulin in total cell extracts from mock- and VP22- transfected cells ...acetylated tubulin increased around 10-fold in VP22-trans- fected cells in comparison to mock-transfected cells ...
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βIII-Tubulin: A novel mediator of chemoresistance and metastases in pancreatic cancer
... βIII- tubulin silencing and induction of apoptosis in pancreatic cancer ...βIII- tubulin is important in providing pancreatic cancer cells with a key survival advantage, thus allowing them to grow and ...
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Revisiting the microtubule based quantum models of mind: tubulin bound GTP cannot pump microtubule coherence or provide energy for alpha <-> beta computation in stable microtubules
... The current paper investigates the biological models of stable brain microtubules as quantum or classical computers whose function is based on electron hopping associated with kinking of the tubulin dimer. ...
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Function of Tubulin Binding Proteins in Vivo
... sis on a 1% agarose gel. This DNA was then transformed into These properties suggest that Rbl2p functions may FSY185 to create a disruption of the entire CIN1 open reading affect processes other than folding of ...
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