Gal(1 → 3)GalNAc(1 → 4)Gal(1 → 4)Glc(1 → 1)Cer
A randomized phase II study to assess the effect of adjuvant immunotherapy using α GalCer pulsed dendritic cells in the patients with completely resected stage II–IIIA non small cell lung cancer: study protocol for a randomized controlled trial
... In total, 56 patients will be included in the study. The primary endpoint is recurrence-free survival, and the secondary endpoints are natural killer T-cell-specific immune response, the frequency of toxic effects and ...
7
Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
... cell function is related to the emergence of exer- cise-induced immunosuppression [4, 5]. Previous studies have reported that activated natural killer T cells (NKT cells) regulate immune responses such as tumor rejection ...
6
Natural killer T cell activation increases iNOS+CD206- M1 macrophage and controls the growth of solid tumor
... Interaction among the NK, NKT cells, and macrophages can shape the immune response [60]. A study by Francesca Bellora et al. showed that activated NK cells could lyse M0 and M2 macrophage while M1 macrophages are ...
13
HIV-1-Specific IgA Monoclonal Antibodies from an HIV-1 Vaccinee Mediate Galactosylceramide Blocking and Phagocytosis
... the Galcer-blocking antibody effects on inhibition of epithelial cell binding of HIV-1 virions and on cell-to-cell transmission of HIV-1 would help determine whether vaccine-elicited IgA plays a critical role in ...
18
Activation of human invariant natural killer T cells with a thioglycoside analogue of ? galactosylceramide
... We also measured the amounts of IL-12 and IL-10 released into supernatants by DC cultured alone or with LPS, α-GalCer or α-S-GalCer or by co-cultures of DC with iNKT cells in the absence or presence of ...
36
Glycolipid antigen induces long term natural killer T cell anergy in mice
... Natural killer T (NKT) cells are a unique subset of T lymphocytes with surface receptors and functional properties shared with con- ventional T cells and NK cells (1–8). Prototypical NKT cells, often referred to as ...
13
α Galactosyl Phytosphingosine 2,6’ Diamide as an Inducer of Invariant Natural Killer T Cell
... chain of iNKT cells (PDB:4EN3). [15] Interestingly, all compounds (1-4) failed to well dock to the site. Only when adopting a site defined by a complex of -GalCer (PDB code: 3SDX) [16] and using a truncated ...
5
Dendritic cells combined with tumor cells and α galactosylceramide induce a potent, therapeutic and NK cell dependent antitumor immunity in B cell lymphoma
... The lack of clinically relevant antitumor efficacy of α-GalCer or DCs loaded with α-GalCer strategies prompted to search for different approaches. We rea- soned that the activation of iNKT cells in the ...
11
Temporal regulation of natural killer T cell interferon gamma responses by β-catenin-dependent and -independent Wnt signaling
... staining was performed on leukocytes using the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer’s instruc- tions. Fc receptor binding was blocked with anti-mouse CD16/32 (2.4G2; BD Pharmingen) before ...
13
Does an NKT-cell-based immunotherapeutic approach have a future in multiple myeloma?
... Different research groups provided evidence of type I NKT cells being quantitatively and qualitatively defective in MM, subsequently hampering their anti- tumor effects [82] (Figure 2). The group of Dhodapkar et al. ...
13
Novel thioglycoside analogs of α galactosylceramide stimulate cytotoxicity and preferential Th1 cytokine production by human invariant natural killer T cells
... To optimize the antitumor properties of human iNKT cells, a number of α -GalCer derivatives and analogues have been synthe- sized to develop compounds, which can selectively induce particular responses in iNKT ...
10
Combination of the toll like receptor agonist and α-Galactosylceramide as an efficient adjuvant for cancer vaccine
... As shown in Fig. 4, Tumors grew rapidly in mice in- oculated with MPL, α-GalCer, pcDNA3, vehicle or PBS, whereas tumor growth was significantly retarded in mice that received DNA vaccine expressing E7 protein with ...
11
Mouse and human iNKT cell agonist β mannosylceramide reveals a distinct mechanism of tumor immunity
... mice (5 mice per group), and glycolipids were administered within 1 hour after tumor challenge. Mice were sacrificed 14–16 days after tumor challenge, and lung metastases were enumerated. (A) Mice were treated with ...
13
Activation of Natural Killer (NK) T Cells during Murine Cytomegalovirus Infection Enhances the Antiviral Response Mediated by NK Cells
... in tumor surveillance. The relevance of NKT cells in immune surveillance of viral infections, however, is not well understood. In this study, we investigated the functional relevance of NKT cells in controlling ...
8
An oral alpha galactosylceramide adjuvanted Helicobacter pylori vaccine induces protective IL 1R and IL 17R dependent Th1 responses
... immunised with either the α -GalCer- or CT-adjuvanted H. pylori antigen formulations (Supplementary Fig. 5d). In contrast, there was a marked difference with regard to induction of protection against challenge. ...
10
Inhibition of iNOS activity enhances the anti-tumor effects of alpha-galactosylceramide in established murine cancer model
... of GalCer was recently evaluated in many animal and clinical studies ...with GalCer also induces the expression of iNOS and enhanced the NO production ...of GalCer in tumor-bearing mice up-regulated ...
12
Alpha galactosylceramide enhances protective immunity induced by DNA vaccine of the SAG5D gene of Toxoplasma gondii
... [32,34,35] and have the capacity to produce high quan- tities of both Th1 and Th2 cytokines. The main immune components affected by α-GalCer administration are specific CD8+ and CD4+ T cells that secrete IFN-γ ...
9
Vaccine-Induced HIV-1 Envelope gp120 Constant Region 1-Specific Antibodies Expose a CD4-Inducible Epitope and Block the Interaction of HIV-1 gp140 with Galactosylceramide
... used Galcer-containing liposomes and HIV-1 Env gp140 proteins as mimics of epithelial cells and HIV-1 virions/HIV-1-infected cells, respectively, to examine the HIV-1 Env-Galcer interaction and the ability ...
12
Alpha galactosylceramide enhances mucosal immunity to oral whole cell cholera vaccines
... This study demonstrates that the addition of the oral adjuvant α-GalCer could enhance mucosal immunogenicity of the inter- nationally most widely licensed OCV, Dukoral ® . Indeed α-GalCer signi fi cantly ...
10
Invariant natural killer T cells are functionally impaired in patients with systemic sclerosis
... Abbreviations α-GalCer: α-Galactosylceramide; Ab: Antibodies; ACA: Anti-centromere antibodies; ANA : Anti-nuclear antibodies; Nucleoli: Anti-nucleoli antibodies; SCL70: Anti-topoisomeras[r] ...
8