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gel electrophoresis (GE)

Agarose Gel Electrophoresis

Agarose Gel Electrophoresis

... Agarose gel electrophoresis is a quick and easy molecular technique used to analyze and separate nucleic acids based on their size ...of). Electrophoresis takes advantage of the fact that DNA’s ...

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Topological patterns in two dimensional gel electrophoresis of DNA knots

Topological patterns in two dimensional gel electrophoresis of DNA knots

... While gel electrophoresis is used very often, and is extremely well char- acterised empirically, there is still no comprehensive theory to quantitatively understand, or predict, what results will be ...

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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles

Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles

... Studies using molecular techniques have demonstrated that a culture-based approach can severely under- estimate the bacterial diversity in most environments. One of the molecular techniques that has been applied in ...

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Pulsed Field Gel Electrophoresis in Differentiation of Erysipelothrix Species Strains

Pulsed Field Gel Electrophoresis in Differentiation of Erysipelothrix Species Strains

... We report here the first analysis of Erysipelothrix spp. using pulsed-field gel electrophoresis (PFGE). Seventy strains of Erysipelothrix spp. were analyzed. SmaI, AscI, and NotI were tested for the ability ...

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Subspecies typing of Vibrio parahaemolyticus by pulsed field gel electrophoresis

Subspecies typing of Vibrio parahaemolyticus by pulsed field gel electrophoresis

... Several molecular methods have been developed for the typing of Vibrio species which are important pathogens in hu- mans and aquacultured animals, for example, ribotyping and pulsed-field gel ...

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Separation of Cancer Proteins using Polyacrylamide Gel Electrophoresis

Separation of Cancer Proteins using Polyacrylamide Gel Electrophoresis

... acrylamide gel electrophoresis and detailed salt fractionation have revealed a number of interesting variations in the size of the globulin fractions in certain disease like multiple ...

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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology

Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology

... gradient gel electrophoresis (DGGE) for the study of total oral microbial populations has been shown previously (4, 23, ...polyacrylamide gel containing a linear gradient of denaturants like ...

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A Review on 2D Gel Electrophoresis:
          A Protein Identification Technique

A Review on 2D Gel Electrophoresis: A Protein Identification Technique

... Two-dimensional electrophoresis (2-D electrophoresis) could be a powerful and wide used technique for the analysis of advanced proteins mixtures extracted from cells, tissues, or alternative biological ...

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Subtyping of Haemophilus influenzaeStrains by Pulsed Field Gel Electrophoresis

Subtyping of Haemophilus influenzaeStrains by Pulsed Field Gel Electrophoresis

... A total of 200 isolates of Haemophilus influenzae were analyzed by serotyping, biotyping, and pulsed-field gel electrophoresis (PFGE). A total of 178 epidemiologically unrelated strains of H. influenzae ...

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One dimensional Polyacrylamide Gel Electrophoresis pdf

One dimensional Polyacrylamide Gel Electrophoresis pdf

... moving-boundary electrophoresis (MBE), discontinuous (disc) elec- trophoresis, isotachophoresis (ITP) and isoelectric fo- cusing ...boundary electrophoresis (MBE), zone electrophor- esis (ZE), disc ...

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Pulsed field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus

Pulsed field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus

... Outbreaks of infections caused by Staphylococcus aureus continue to be a problem for health care facilities (16). Iden- tifying strains among the outbreak isolates is a major step in determining the source of the ...

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Evaluation of Pulsed Field Gel Electrophoresis Profiles for Identification of Salmonella Serotypes

Evaluation of Pulsed Field Gel Electrophoresis Profiles for Identification of Salmonella Serotypes

... Traditionally, phenotypic methods such as serotyping have been used for identification of Salmonella isolates in outbreak investigations. However, phenotypic methods have limited util- ity for epidemiologic analysis of ...

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Rapid Discrimination of Listeria monocytogenes Strains by Microtemperature Gradient Gel Electrophoresis

Rapid Discrimination of Listeria monocytogenes Strains by Microtemperature Gradient Gel Electrophoresis

... gradient gel electrophoresis ( ␮ -TGGE) was examined for use for the rapid subtyping of Listeria monocytogenes ...total electrophoresis time of only 7 ...

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Rapid Pulsed Field Gel Electrophoresis Protocol for  Subtyping of Campylobacter jejuni

Rapid Pulsed Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni

... pulsed-field gel electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial ...

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Identification of the multiple beta thalassemia mutations by denaturing gradient gel electrophoresis

Identification of the multiple beta thalassemia mutations by denaturing gradient gel electrophoresis

... gradient gel electrophoresis to detect the beta-thalassemia mutations in the Chinese ...gradient gel electrophoresis offers a nonradioactive means of detecting multiple mutations in genetic ...

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Two–Dimensional Gel Electrophoresis of Ceolomic Fluid of Eisenia foetida Earthworm

Two–Dimensional Gel Electrophoresis of Ceolomic Fluid of Eisenia foetida Earthworm

... two-dimensional gel electrophoresis of coelomic fluid protein of earthworm were performed using ...12.5% gel, topped by a 7% stacking gel prepared based on the method proposed by Laemmli ...

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Proteins – structure, properties and their separation by SDS-polyacrylamide gel electrophoresis

Proteins – structure, properties and their separation by SDS-polyacrylamide gel electrophoresis

... a gel is ...the electrophoresis. After loading the samples into the wells of the gel an electric field is applied across the ...gel. Electrophoresis is usually carried out under ...

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Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed field gel electrophoresis

Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed field gel electrophoresis

... Pulsed-field gel electrophoresis (PFGE) has shown success in typing enterococci and has facilitated the study of local out- breaks and dissemination of antibiotic resistance among en- terococci ...

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Confidence bands for inverse regression models with application to gel electrophoresis

Confidence bands for inverse regression models with application to gel electrophoresis

... In Section 2 we introduce the basic assumptions and present our asymptotic results for con- structing confidence bands. However, it is well-known that convergence in the resulting limit theorems is rather slow (Hall ...

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Increase in local protein concentration by field-inversion gel electrophoresis

Increase in local protein concentration by field-inversion gel electrophoresis

... Pulsed-field gel electrophoresis (PFGE) is an elegantly simple and universally accepted technique for the separa- tion of large DNA molecules ...field-alternation gel electrophoresis (OFAGE) ...

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