Gene Cloning, Expression and Purification.
Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... It was demonstrated that supplementing BCG with Mtb secreted protein subunit vaccines enhanced protective immune responses against TB in animal models (4, 16, and 17). Several studies investigated the efficacy of fusion ...
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THE GENE CLONING, OVEREXPRESSION, PURIFICATION, AND CHARACTERIZATION OF DIBENZOTHIOPHENE MONOOXYGENASE AND DESULFINASE FROM GORDONIA ALKANIVORANS RIPI90A
... strong expression of recombinant dszA and dszB genes separately to obtain a high level of active ...the expression of the Dsz enzymes in recombinant strain resulted in a great amount of insoluble enzymes ...
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New Tannase-Producing Lactobacillus Sp. Nrc10: Gene Cloning, Enzyme Purification, And Characterization
... –coding gene was amplified using the PCR technique. The cloning of tannase gene (Tanlp1) was carried out and its complete sequence was ...The cloning and efficient expression of tannase ...
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Cloning, expression, purification and characterization of two diagnostically important proteins of Brucella abortus
... encoding gene was cloned in pET 28a(+) vector using XhoI and NcoI restriction sites, while hypothetical (20 kDa) protein encoding gene was cloned in pQE-30 UA expression vector as per the ...
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4-Dihydrotrisporin-Dehydrogenase, an Enzyme of the Sex Hormone Pathway of Mucor mucedo: Purification, Cloning of the Corresponding Gene, and Developmental Expression
... CsgA gene from the myxobacterium Myxococcus xanthus was found to be the closest relative, with 31 to 35% sequence identity between CsgA and the mucoralean ...CsgA gene from ...
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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus
... Colonies from each plate were selected to produce cell cultures from which to analyze the cloned plasmids. Twelve to twenty-four different cultures containing a single colony were grown separately in 5 mL LB media ...
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The vaccinia virus H5R gene encodes late gene transcription factor 4: purification, cloning, and overexpression.
... H5R gene product the name viral late gene transcription factor 4 ...Its expression pattern and subcellular distribution suggest that the H5R gene product may have multiple roles in the viral ...
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Cloning and in Vitro Expression of the Human Alpha‑Fetoprotein Gene
... Thus, biological features of the AFPh offers great opportunities to develop various anticancer drugs that could be highly selective to the tumor cells. However, this promising perspective is limited to the complexity and ...
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Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
... single gene, encod- ing the potential epitopes of three antigens of ...after cloning into an expression vector, the expressed and purified protein was used for evaluation of immunoreactivity of the ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... pET expression system has been developed for the cloning and ex- pression of recombinant proteins in ...sub cloning of 820 bp fHBP gene into pET 28a (+) expression vec- tor was carried ...
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CLONING, SEQUENCING AND EXPRESSION OF THE OVINE INTERLEUKIN-6 GENE
... (A) Proteins from various stages of IL-6 purification were analysed by electrophoresis on SDS-15% polyacrylamide gels, followed by staining with Coomassie blue R. Lane M: marker proteins. Lane 1: total proteins ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... The restriction digestion was performed using 10U BamHI and 20U SalI for ESAT-6; 10 U BamHI and 20 U HindIII forCFP-10, 1 µg DNA and 1XBamHI Buffer (10 mmol ⁄ LTris–HCl (pH 8.0 at 37°C), 5 mmol ⁄ L MgCl 2 ,100 mmol ⁄ L ...
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CLONING AND EXPRESSION OF LEISHMANOLYSIN GENE FROM LEISHMANIA MAJOR IN PRIMATE CELL LINES
... further purification of these clones, digestion of the corresponding plasmids with Hind III and Bam HI gave rise to a 1443 bp band related to the Leishmanolysin ...
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Cloning and expression of human vasohibin1 gene in E. coli
... pET expression system is one of the common systems of cloning and expression of recombinant proteins in ...VASH1 gene was cloned into pET28a expression vector and transformed into ...
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Cloning and Expression of a Novel Phytase Gene (phyMS) from Mycobacterium smegmatis
... phyMS gene from pMSuia plasmid was outsourced to Macrogen (South Korea), and sequencing was conducted at both ends using pBAD priming, TamMSF1 and ...phyMS gene was in frame with the DNA sequence coding for ...
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Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
... In this study Clav-MO fused to thioredoxin in its N-terminal presented antibacterial activity. Although one could argue that thioredoxin could have inhibitory activity by itself its gene sequence was from the E. ...
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CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE
... nifK gene was cloned and the protein was induced to lay foundation for further studying the molecular mechanism of strain DX120E promoting sugarcane growth, antibody preparation, enzyme activity in vitro and ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... of gene in pGEM-T Easy vector. The presence of insert gene was confirmed by gel mobility analysis as compared to control DNA isolated from blue ...pET32a expression system was ...for ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... From acetic acid bacteria were purified and character- ised several DNA plasmids which encoded rep gene which product is able to regulate replication process. The first identified cryptic plasmid from Acetobacter ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... polymerase gene was subcloned to pET301/CT DEST vector (Invitrogen, USA) by a recombination ...polymerase gene in the destination constructs was confirmed by PCR, using primers designed according to the ...
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