gene probe
Use of a pilin gene probe to study molecular epidemiology of Pseudomonas aeruginosa
... Multiple morphologically different isolates from individual patients with CF were studied; each isolate in 33 of 40 sputum samples had an identical RFLP type, despite considerable LPS se[r] ...
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Simplified and accurate nonradioactive polynucleotide gene probe assay for identification of enterotoxigenic Escherichia coli
... Cloned polynucleotide and synthetic oligonucleotide probes used in colony hybridization are equally efficient in the identification of enterotoxigenic Escherichia coli.. Comparative stud[r] ...
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Molecular cloning of Ehrlichia risticii and development of a gene probe for the diagnosis of Potomac horse fever
... Autoradiogram of the dot blot carrying the DNA from peripheral blood mononuclear cells collected from an experimentally infected horse on different days p.i.. listed to the left.[r] ...
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Distinct gene subsets in pterygia formation and recurrence: dissecting complex biological phenomenon using genome wide expression data
... Comparison of microarray data with tear proteins Another report from our center has previously docu- mented the analysis of tear proteins in tears of patients after surgery for pterygium.[18] Briefly, this study employed ...
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Simultaneous Detection and Differentiation between Wild Type and Vaccine Measles Viruses by a Multiplex Real Time Reverse Transcription PCR Assay
... gene probe. However, since the sensitivity for genotype D8 using the L gene probe was 2 copies/reaction and the H gene assay result is used for differentiation between vaccine and ...
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The akuBKU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus
... introduced into strain KU80 ⌬ pyrG. Transformants were FIG. 2. Calcineurin A deletion mutant ( ⌬ calA) showed increased branching and a phenotype of reduced growth. (A) Southern blot analysis of the ⌬ calA strain. ...
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Sex Pheromone Response, Clumping, and Slime Production in Enterococcal Strains Isolated from Occluded Biliary Stents
... structural gene for the pAD1-encoded AS revealed the pres- ence of two Arg-Gly-Asp motifs (30), recognized by integrins, a family of eukaryotic receptors expressed on leukocytes, thrombocytes, and endothelial and ...
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PCR Detection and Molecular Identification ofChlamydiaceae Species
... family-specific probe (CfamP). The species-specific probe for ...(CtraP) probe detected ...family-specific probe used with ...species-specific probe indicates that the signal reduction ...
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Conserved sequences of the adenovirus genome for detection of all human adenovirus types by hybridization
... The hexon gene, containing the most homologous adenovirus sequences, constitutes the best single sequence of the adenovirus genome for use as a cross-reactive probe for the diagnosis of [r] ...
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Real-time PCR TaqMan assay for rapid screening of bloodstream infection
... blood culture is the standard method for the diagnosis of bacteremia. However, the final results from identification/ antimicrobial susceptibility tests in the continuous monitor- ing blood culture system (CMBCS) require ...
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A general framework for optimization of probes for gene expression microarray and its application to the fungus Podospora anserina
... a gene expression microarray comprises several time-consuming and complex ...steps. Probe librar- ies are generated by commercial services or specialized design programs [1], which analyze nucleic acid ...
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Variation in fiberoptic bead-based oligonucleotide microarrays: dispersion characteristics among hybridization and biological replicate samples
... cates are available and not quite reliable for triplicates (URL address for the RMAExpress is http://stat.berke ley.edu/~bolstad/RMAExpress/RMAExpress.html). The high level analysis consists of two basic steps: normal- ...
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Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe
... Clinically isolated B. anthracis may be associated with either commensal or pathogenic bacteria. To determine whether the BAGYRA1668 MGB probe would cross-react with other bac- teria, we tested it with 43 strains ...
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Identification of toxigenic Clostridium difficile strains by using a toxin A gene specific probe
... A 4.5-kilobase PstI fragment encoding part of the toxin A gene was isolated and used as a DNA probe in colony hybridization studies with 58 toxigenic and 17 nontoxigenic Clostridium diff[r] ...
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MM1, a Temperate Bacteriophage of the Type 23F Spanish/USA Multiresistant Epidemic Clone of Streptococcus pneumoniae: Structural Analysis of the Site-Specific Integration System
... (strain 8249, an important multiresistant strain, originally iso- lated in South Africa), 14 (CSUB 3409), 9V (CSUB 4086), 33C (SSISP33C/1), and 33F (SSISP33F/1). A first experimental ap- proach was based on the ...
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Designed and Analyzing Different Conical Probes for Detecting Tumor in Skin
... dielectric probe with different radius design has 2mm probe, 3mm probe, 4mm probe and 5 mm probe antennas which are analyzed at 1W input power and various parameters like Electric Field ...
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Identification of Small and Discriminative Gene Signatures for Chemosensitivity Prediction in Breast Cancer
... 13 probe set signature highlighted the critical genes for response prediction, and therefore provided clear insight into the molecular mechanisms that regulate the chemosensitivity in breast ...13 probe ...
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The feasibility study of transnasopharyngeal esophagus echocardiography in the ultrasonic diagnosis
... ultrasound probe for TNPEE. Philips S8-3 t probe is a special transesophageal probe for ...the probe is thin and soft, it is only suitable for some ...the probe can not pass through the ...
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Vaccinia virus-encoded ribonucleotide reductase: sequence conservation of the gene for the small subunit and its amplification in hydroxyurea-resistant mutants.
... A mixed oligonucleotide probe for the VV M2 gene was designed based on an examination of the predicted amino acid sequences of four ribonucleotide reductase small-subunit proteins: Esche[r] ...
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Comparison of <em>MIC-3</em> Protein Accumulation in Response to Root-knot Nematode Infection in Cotton Lines Displaying a Range of Resistance Levels
... MIC gene is involved in the resistance response to ...MIC-3 gene to other genes of known or unknown function in the major gene databases and the lack of any clues from its structure ...MIC ...
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