Generating cell-lines stably expressing V5-TEV-PP7
Functional expression of native and mutant Y1 receptors in stably transfected adenocarcinoma cell lines
... In stably transfected systems, influences on the long-term regulation of the receptor must also be ...clones expressing the native Yi or the Yi(S255E) receptors, a number of questions should be ...
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GnRH receptor activation competes at a low level with growth signaling in stably transfected human breast cell lines
... cancer cell lines expressing high levels of the GnRH receptor, despite eliciting robust signalling, pro- vides a valuable tool for determining the intracellular context which does (eg HEK 293 and ...
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Adenovirus type 5 precursor terminal protein-expressing 293 and HeLa cell lines.
... 293 cell lines that express biologically active adenovirus type 5 precursor terminal protein (pTP) have been ...these cell lines ranges from barely detectable to greater than that observed in ...
7
Design and analysis of stably integrated reporters for inducible transgene expression in human T cells and CAR NK-cell lines
... Chimeric antigen receptors (CARs) are engineered molecules that endow the immune effector cells, typically T cells, with the ability to recognize a pre-defined surface epitope and des- troy the cognate cell in an ...
9
Restoration of interferon responses of adenovirus E1A-expressing HT1080 cell lines by overexpression of p48 protein.
... E1A-expressing cell lines derived from the HT1080 cells, neither IFN- a nor IFN- g could induce the transcription of genes containing the IFN-stimulated response element ...by stably ...
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Gene Expression Profiles are Altered in Human Papillomavirus 16 E6 D25E Expressing Cell Lines
... we stably transduced the E6 expression vector into C33A ...Cells expressing the E6 protein were selected and maintained in medium containing ...variant- expressing C33A cells. In both cell ...
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Overexpression of G6PDH does not affect the behavior of HEK-293 clones stably expressing interferon-α2b
... and cell growth. The PYC cell line was expected to amplify and benefit from the effect of G6PDH overexpression because of the “pull” effect of PYC from glycolysis downstream toward the TCA ...both ...
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Feasibility of Generating Adeno-Associated Virus Packaging Cell Lines Containing Inducible Adenovirus Helper Genes
... packaging cell lines that require Ad with E1A, E1B, and E3 ...E1-expressing cell line E1-112, we set out to test whether the AAV Rep genes as well as the Cap genes can be stably ...
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Inhibition of human immunodeficiency virus type 1 replication in human T cells stably expressing antisense RNA.
... In cell lines with antisense RNA expression detectable by Northern (RNA) hybridization, human immunodeficiency virus type 1 replication was inhibited to 88% 10 days postinfection and thi[r] ...
5
Adenovirus Vector Pseudotyping in Fiber-Expressing Cell Lines: Improved Transduction of Epstein-Barr Virus-Transformed B Cells
... packaging lines expressing dif- ferent fiber ...single cell type would involve eliminating their binding to the natural fiber receptor ...than generating a new viral chromosome each time a ...
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Generation and analysis of clonal IgM and IgG producing human B cell lines expressing an anti DNA associated idiotype
... for generating stable, cloned, EBV-transformed IgG- and IgM-producing human B cell ...these lines we have characterized immunoglobulin V gene utilization in an anti-DNA-associated idiotypic ...these ...
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Inducible expression of encephalomyocarditis virus 3C protease activity in stably transformed mouse cell lines.
... Consistent with these results, examination of total labeled proteins in lysates prepared from superinduced 120 cells showed that of those polypep- tides resolvable in a one-dimensional a[r] ...
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Effectiveness of malabaricone-A in P-glycoprotein over-expressing cancer cell lines
... over- expressing MDR+ cancer cell line, this study was extended to a broader panel of leukemic and multiple myeloma cell ...cancer cell lines and the cytotoxicity of MAL-A was evaluated ...
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Generating Rho-0 cells using mesenchymal stem cell lines
... Results are expressed as percent of positive cells for each dye and represent the mean of three independent experiments (mean ± SD). Mitochondrial respiration Oxygen consumption (OCR, pmoles/min), an indicator of ...
23
Radiosensitivity of human breast cancer cell lines expressing the breast tumor kinase (Brk)
... increased cell survival, enhanced cellular migration and elevated proliferation [7]; however, the impact of Brk expression on cellular radiation sensitivity and DNA DSB repair has not been ...key cell cycle ...
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Rapid Communication. Magnetic Cell Sorting Purification of Differentiated Embryonic Stem Cells Stably Expressing Truncated Human CD4 as Surface Marker
... To establish MACS for genetically engineered ES cells, we generated a ΔCD4EGFP fusion construct simultaneously offer- ing the advantages of in vivo detectability via green fluorescence. Our work shows that even more than ...
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Generating Story Reviews Using Phrases Expressing Emotion
... ・別れの場面が悲しかった。 (The parting scene is sad. ) Review sentences exist in review articles. We decide on a rule for extracting them heuristically; the rule is based on specific nouns, which exhibit the point of the scene in ...
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2G12-Expressing B Cell Lines May Aid in HIV Carbohydrate Vaccine Design Strategies
... The highly conserved cluster of high-mannose glycans on the HIV-1 envelope glycoprotein, gp120, has been highlighted as a tar- get for neutralizing antibodies. 2G12, the first HIV-1 antiglycan neutralizing antibody ...
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In vitro and in vivo properties of CD133 expressing cells from human lung cancer cell lines
... μm cell-strainer cap (BD Falcon, USA) and then kept on ...analysis. Cell sorting was performed on a FACSDiva cell sorter (Flow Vantage machine, Becton ...
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Human small cell lung cancer cell lines expressing the proopiomelanocortin gene have aberrant glucocorticoid receptor function
... in addition, they did not suppress chloramphenicol acetyltransferase expression from (TRE)3-tkCAT. Similar results were obtained with two other POMC-expressing SCLC cell lines. Expression of wild ...
7