Genome mapping and sequencing thunder mutation
Isolation of Temperature-sensitive Mutants in the Moss Physcomitrella patens and Mapping of their Causal Mutation by Genome Sequencing of Pooled Segregants
... causal mutation (Zuryn et ...causal mutation can then be determined (Zuryn et al., 2010). Mapping by pooled-sequencing of segregants, instead, requires a relatively large population of ...
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Nanopores and Nanochannels: From Gene Sequencing to Genome Mapping
... label-free sequencing principle has been commercially launched as a $1000-priced palm-sized device that is expected to help democratize ...of genome mapping with nanochannels, and relates it to DNA ...
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A Strategy for Direct Mapping and Identification of Mutations by Whole-Genome Sequencing
... reference genome do not represent an issue for mutation identification using our strategy, as the mutant genomes are compared between each other for identification and removal of background ...of ...
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Whole-Genome Restriction Mapping by “Subhaploid”-Based RAD Sequencing: An Efficient and Flexible Approach for Physical Mapping and Genome Scaffolding
... the genome should contain 0–1 polymorphic loci which would not affect the tag identity and mapping ...PacBio sequencing and assem- bly of fosmid/BAC dilution pools followed by Hi-C-based ...
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Full genome re sequencing reveals a novel circadian clock mutation in Arabidopsis
... successfully mapping to the reference, leading to lower coverage overall, hence more loci being discarded because cover- age was too low for one or other of the ...
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A comprehensive assessment of somatic mutation detection in cancer using whole genome sequencing
... and sequencing with two independent platforms (MiSeq and ...and sequencing on these other platforms, which led to a low rate of independently validated ...different sequencing centres, which we ...
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Finding a needle in a haystack: whole genome sequencing and mutation discovery in murine models
... in sequencing a murine genome may not be relevant to disease patho- ...whole genome sequencing data were com- pared with sequencing data from matched germline DNA to assess whether ...
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A comprehensive assessment of somatic mutation detection in cancer using whole-genome sequencing
... and sequencing with two independent platforms (MiSeq and ...and sequencing on these other platforms, which led to a low rate of independently validated ...different sequencing centres, which we ...
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Quantifying the mapping precision of genome wide association studies using whole genome sequencing data
... and mapping precision at these loci can be improved in the future with larger sample ...in mapping precision between different SNP genotyping arrays were ...trivial. Mapping pre- cision of GWAS using ...
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Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans
... causal mutation is not as straightfor- ward because rescue with the wild-type copy is often not ...a mutation causes a dominant phenotype due to haploinsufficiency (a situation when one wild-type copy is not ...
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Detecting somatic point mutations in cancer genome sequencing data: a comparison of mutation callers
... As demonstrated in the section above, when calling sSNVs, another potential source of false-positives is strand bias. Here, we specifically call an sSNV whose al- ternate alleles all come from one strand a strand-biased ...
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Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome
... the mutation of ...Generation Sequencing (NGS) technologies [17-19], the possibilities to improve the efficiency of reverse- genetic screening have ...a genome size of 373 Mb [2], ...
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Efficient Mapping and Cloning of Mutations in Zebrafish by Low-Coverage Whole-Genome Sequencing
... Whole-genome sequencing has recently emerged as a fast and ef fi cient approach for identifying mutations in nonvertebrate model ...large genome and lack of fully inbred ...ciently mapping and ...
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Mapping by Sequencing the Pneumocystis Genome Using the Ordering DNA Sequences V3 Tool
... Davidson, S. B., J. Crabtree, B. Brunk, J. Schug, V. Tannen et al., Miller, J. A., D. Palaniswami, A. P. Sheth, K. J. Kochut and H. 2001 K2/Kleisli and GUS: experiments in integrated access to Singh, 1998 WebWork: ...
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Old can be new again: HAPPY whole genome sequencing, mapping and assembly
... both genome mapping and sequencing methods have ad- vanced significantly to provide a foundation for scientists to understand genome structures and functions in many ...speaking, genome ...
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Whole genome sequencing reveals a de novo SHANK3 mutation in familial autism spectrum disorder
... point mutation in three siblings with Autism Spectrum ...B) Mutation as evidenced by whole genome sequencing compared to reference sequence (GRCh37) at ...Capillary sequencing ...
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Genome‐wide linkage analysis and whole‐exome sequencing identifies an ITGA2B mutation in a family with thrombocytopenia
... R1026W mutation (referred to as R995W in some reports) is thought to result in constitutive activation of the aIIbb3 receptor (Kunishima et al, 2011) and has been previously reported in 7 Japanese families with AD ...
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Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome
... the genome that is cut to a PCR-amplifiable fragment in at least one of the ...the genome is highly likely then to be repre- sented by a fragment 1-3 kb in size in at least one of these parallel ...
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Whole-genome sequencing of bladder cancers reveals somatic CDKN1A mutations and clinicopathological associations with mutation burden
... whole-genome sequencing to identify somatic mutations and chromosomal changes in 14 bladder cancers of different grades and ...and/or mutation burden within early-stage or low-grade tumours could ...
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Genome sequencing and crop improvement
... unassembled genome sequence data and expressed sequence tags ...reference genome sequence, genetic mapping of these markers is required to determine their genomic ...
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