Genotyping using allele specific PCR
Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors
... Allele-specific PCR experiment for genotyping errors elimination Pre-PCR treatment by betaine and modifying AS-PCR program on mixed DNA template sample model and clinical samples ...
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Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene
... design specific primers and ...effective genotyping method for variants with this type of ...out genotyping for detecting SNPs, some using automated systems developed for high throughput ...is ...
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Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR
... While allele-specific PCR has been around for many years, its adoption for SNP genotyping or somatic mutation detection has been hampered by its low discriminating power and high ...of ...
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An improved allele-specific PCR primer design method for SNP marker analysis and its application
... SNP genotyping methods such as CAPs (The Cleaved Amplified Polymorphic Sequence), dCAPs (derived CAPS), and AS-PCR (Allele-specific PCR) are widely used for low-throughput applications ...
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Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR
... Background Chloroplast DNA is a primary source of molecular varia- tions for phylogenetic analysis of photosynthetic eukary- otes. The chloroplast genome has a simple and stable genetic structure, it is haploid, there ...
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T ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
... T-ARMS PCR is flexible, rapid and economical SNP detection tool compared to contemporary genotyping tools such as allele-specific PCR (AS-PCR) [1], high- resolution melting ...
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Simple allele-discriminating PCR for cost-effective and rapid genotyping and mapping
... the PCR annealing temperature is ...MT allele-specific primers are best kept at similar length to allow for same PCR con- ...Finally, PCR conditions have to be first optimized ...
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Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species
... of allele-specific amplification, single nucleotide artificial mismatches were introduced at the third nucleotide from the 3' end of the ...tested using isolated genomic DNA and 10 different ...
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Staphylococcus aureus Genotyping Using Novel Real Time PCR Formats
... teria for calling SNPs and the presence or absence of binary genes. The criteria were different for each SNP, reflecting differences in the relative priming efficiencies of the allele- specific primers. In ...
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Original Article An allele specific locked nucleic acid real time quantitative PCR for detection of HBV rtA181V and rtN236T mutations
... of PCR, LNA-modified nucleotides have been widely used for a variety of applica- tions, including SNP genotyping ...LNA PCR to quan- tify rtA181V and rtN236T mutations associat- ed with ADV ...By ...
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Measles Virus Genotyping by Nucleotide Specific Multiplex PCR
... simple genotyping method based on multiplex PCR has been developed to discriminate between all active measles virus (MV) clades and genotypes (A, ...
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Allele specific PCR detection of sweet cherry self incompatibility alleles S3, S4 and S9 using consensus and allele specific primers in the Czech Republic
... The PCR-based detection of S-allele helps to promote and speed up traditional breeding activity and hence molecular analysis of the perspective genotypes has become more intensive in all cherry growing ...
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Rapid and Specific Genotyping System for Hepatitis B Virus Corresponding to Six Major Genotypes by PCR Using Type Specific Primers
... The genotyping of HBV is important to clarify the route and pathogenesis of the ...genotype-specific PCR primers, it is well established that not only higher matching in the entire sequences but also ...
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Allele specific real time PCR testing for minor macrolide resistant Mycoplasma Pneumoniae
... and Ji et al. [21], which might indicate ASPCR is a highly sensitive, accurate, time-saving and high throughput method for detecting resistant M. pneumoniae. Of the 164 M. pneumoniae positive samples, 61.59% had the ...
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Rapid determination of clonality by detection of two closely linked X chromosome exonic polymorphisms using allele specific PCR
... (13), PCR-ASOH (14) and PCR amplification of specific alleles (PASA) ...retaining allele-specific ...h; PCR-LDR can take as long as 2 or 3 ...same PCR conditions are ...
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Detection of Ethambutol-Resistant Associated Mutations in Mycobacterium tuberculosis Isolates from Iran Using Multiplex Allele-Specific PCR
... and PCR restriction fragment polymor- phism (PCR-RFLP) have been extensively used for detection of EMB resistance- related ...mutiplex allele-specific (MAS)-PCR has received a great ...
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Hepatitis C virus replicative levels and efficiency of genotyping by specific PCR and antibody assay
... logical genotyping, which is based on a comparison of the different antibody responses elicited by different HCV geno- types, is necessarily ...serological genotyping should be interpreted with caution, ...
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Detection of BRAF mutation in thyroid papillary carcinomas by mutant allele-specific PCR amplification (MASA)
... a specific, sensitive and reliable method to detect BRAF transversion mutation in small quantities of DNA extracted from different sources, including cytologic material obtained either fresh or from archival glass ...
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Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1
... Interesting epidemiological indications could be derived from genotyping of the P. vivax isolates. Malaria is consid- ered to be hypoendemic in Thailand where transmission, confined to regions bordering Cambodia, ...
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DETECTION OF HUMAN INTESTINAL PROTOZOA BY USING MULTIPLEX ALLELE SPECIFIC POLYMERASE CHAIN REACTION (MAS-PCR) In New Damietta City
... Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) assay and microscopy for detection and identification of common pathogenic protozoan parasites in New Damietta city of North Delta ...
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