Gfp fusion
GFP Fusion Proteins: A Solution or a Problem?
... microscopy. GFP tagged proteins can be used to track and examine real time localization, interactions and translocation of proteins of interest as well as to investigate several aspects of cell ...unusual ...
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A GFP-fusion coupling FACS platform for advancing the metabolic engineering of filamentous fungi
... a GFP-fusion coupled fluorescence-activated cell sorting (FACS) platform was established to speed up the build and test process of the DBTL cycle, by enabling rapid selection for expressible heterologous ...
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Active inclusion bodies of acid phosphatase PhoC: aggregation induced by GFP fusion and activities modulated by linker flexibility
... the GFP domain in PhoC-GFP fusion protein can be rea- sonably supposed to fold less efficiently and rapidly than non-fusion GFP, presumably prolonging the intermediate- folding time ...
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Structural stability and endonuclease activity of a PI-SceI GFP-fusion protein
... PI-SceI GFP-fusion protein structure, SDS-PAGE of purified proteins, and illustration of PCR steps performed to create and amplify the PI-SceI-GFP encoding ...of GFP and PI-SceI intein bound ...
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Subcellular Localization of Transmembrane E cadherin GFP Fusion Protein and Expression in HeLa Cells
... C-terminal GFP tagged GFP-E- cadherin fusion protein localization in the transmembrane of the ...of GFP-E-cadherin fusion protein was studied followed by relative quantification of the ...
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Design, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae
... HOG1::GFP fusion protein, led to a persistent increase in the level of nuclear accumulation of HOG1, in contrast to salt stress, which—as expected—showed the well-characterized transient ...
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Human Cytomegalovirus UL97 Kinase Activity Is Required for the Hyperphosphorylation of Retinoblastoma Protein and Inhibits the Formation of Nuclear Aggresomes
... hypothesis, GFP-GCP170* was co- expressed with pp71 and pUL69 in COS7 cells to see whether they exhibited similar staining ...with GFP-GCP170*, suggesting that FIG. 2. Inhibition of pp71 and ...
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Farnesyl diphosphate synthase, the target for nitrogen-containing bisphosphonate drugs, is a peroxisomal enzyme in the model system Dictyostelium discoideum
... FDPS–GFP fusion protein was expressed in mammalian cells but it was not possible to determine whether it was located in the peroxisomes or in the cytosol ...
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Immobilisation of active enzymes on novel GFP protein particles : a thesis submitted in complete fulfilment of the requirements of the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand
... for GFP fluorescence. GFP fluorescence is generated by a hexapeptide chromophore consisting of the amino acids Phe64-Ser65-Tyr66-Gly67-Val68-Gln69 with autocatalytic cyclisation occurring between ...
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Evidence of a Continuous Endoplasmic Reticulum in the Protozoan Parasite Entamoeba histolytica
... Untargeted GFP was present diffusely in the cytosol, including within pseudopodia ...geted GFP fusion protein localized to a widely distributed reticular ...fluorescent fusion protein from ...
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Polycomb group protein complexes exchange rapidly in living Drosophila
... on GFP fusion proteins of Polycomb (Pc) and Polyhomeotic (Ph), two essential members of the PCC in whole living Drosophila embryos and larval tissues to determine their diffusion, binding equilibria and ...
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A 38-Amino-Acid Sequence Encompassing the Arm Domain of the Cucumber Necrosis Virus Coat Protein Functions as a Chloroplast Transit Peptide in Infected Plants
... CP GFP fusion ...the GFP fusion ...The GFP in these constructs [with the exception of GFP/arm( ⫹ SVRI)/CNVexp] was His tagged at the carboxyl terminus ...of fusion ...
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Ilkavets, Iryna (2006): Membrane proteins in the outer mebrane of plastids and mitochondria. Dissertation, LMU München: Fakultät für Biologie
... C-terminal fusion of GFP to the open reading frame of the pea VDAC gene was ...the GFP-fusion protein was bombarded in pea roots. The GFP-signal was monitored by fluorescence ...VDAC– ...
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Import-Associated Translational Inhibition: Novel In Vivo Evidence for Cotranslational Protein Import into Dictyostelium discoideum Mitochondria
... of GFP: (i) reduced expression of the fusion genes, ...targeted fusion proteins may be limited due to an inter- action between the processes of translation and mitochondrial import (for such ...
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Spectral properties of LH2 exhibit very similar even when heterologously express LH2 with β subunit fusion protein in Rhodobacter sphaeroides
... β-subunit-GFP fusion protein complexes and quantified, respectively (Figure ...hand, GFP should not be con- sidered as the acceptor of the ...β-subunit-GFP fusion protein complexes ...
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Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris
... the GFP and ROL genes were fused using the complementary linker regions as PCR ...ROL-GFP fusion, DR10 and DR11 were utilized in GFP-ROL ...resulting fusion DNA fragments, ...
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Regulation of Physiological Rates in Caenorhabditis elegans by a tRNA-Modifying Enzyme in the Mitochondria
... We show that the phenotype associated with gro-1(e2400) comprises the whole suite of features that characterize the phenotype of the clk mutants in Caenorhabditis elegans, including deregulated develop- mental, ...
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A Pmk1-Interacting Gene Is Involved in Appressorium Differentiation and Plant Infection in Magnaporthe oryzae
... PIC5-GFP fusion constructs and transformed them into the pic1 and pic5 knockout mutants GA22 and ML4, ...PIC5-GFP fusion constructs, 118-kDa and 56-kDa bands of the predicted Pic1-GFP ...
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Extended C termini of CPC LIKE MYB proteins confer functional diversity in Arabidopsis epidermal cell differentiation
... C-GFP fusion proteins in the root epidermal cells was addressed by immunoblot analysis of the proteins extracted from the root tissue of wild-type (Col-0), and CPC:TRY-GFP and CPC: TRYΔC-GFP ...
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Two Dictyostelium Orthologs of the Prokaryotic Cell Division Protein FtsZ Localize to Mitochondria and Are Required for the Maintenance of Normal Mitochondrial Morphology
... Fsz-GFP fusion proteins may have a reduced ability to function normally and fully complement the losses of the native ...the GFP- tagged Fsz proteins are still capable of polymerizing into struc- ...
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